Study of antibacterial and antifungal activity of traditional Cedrus deodara and Pinus roxburghii Sarg

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Chaudhary, Amrendra Kumar;Ahmad, Shamim;Mazumder, Avijit

  • 투고 : 2012.09.26
  • 심사 : 2012.11.21
  • 발행 : 2012.11.30

초록

In the present study, the volatile oil, chloroform extract and methanol extract of the woods of the plants Cedrus deodara and Pinus roxburghii were screened for their antibacterial and antifungal activities against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis, Streptococcus pyogenes, Candida albicans, Aspergillus niger, and Aspergillus clavatus using the Agar diffusion method. The susceptibilities of the microorganisms to the extracts were compared with each other and with a selected standard antibiotic. It was observed that the volatile oil and chloroform extracts showed the significant antibacterial activities while the least antibacterial activity was recorded with the methanolic extracts. The higher Minimum Inhibitory Concentration value of the extracts and oils against fungus suggested that the plants may possess less antifungal activity. Phytochemical analysis and thin-layer chromatography profiling revealed the presence of flavonoids and terpenoids in the oil and chloroform extracts, which could explain the antimicrobial activity. The findings suggest that the Cedrus deodara and Pinus roxburghii have antimicrobial properties and they can be used in the treatment of infectious diseases. However, further work is required in order to isolate the active constituents of the plants responsible for the antibacterial activity.

키워드

Cedrus deodara;Pinus roxburghii;antimicrobial;flavonoids;terpenoids;ampicilin

참고문헌

  1. Agrawal PK, Agarwal SK, Rasgi RP. Dihydroflavonoids from Cedrus deodara. Phytochemistry. 1980;19:893-896.
  2. Barry AL, Brown SD. In-vitro studies of two triazole antifungal agents (voriconazole [UK-109,496] and fluconazole) against Candida species. Antimicrob Agents Chemother. 1996;40:1948-1949.
  3. Cushnie TP, Lamb AJ. Antimicrobial activity of flavonoids. Int J Antimicrob Agents. 2005;26:343-356. https://doi.org/10.1016/j.ijantimicag.2005.09.002
  4. Handa SS, Khanuja SP, Longo G, Rakesh DD. Extraction technologies for Medicinal and Aromatic Plants. (Italy: International Centre for Science and High Technology), pp. 42-44, 2008.
  5. Hussain M, Shah GM, Khan MA. Traditional Medicinal and Economic uses of Gymnosperms of Kaghan Valley, Pakistan. Ethnobotanical Leaflets. 2006;10:72-81.
  6. Kirtikar KR, Basu BD. Indian medicinal plants. Vol-III. (Dehradun, India: International Book Distributors), 2006.
  7. Kunwar RM, Uprety Y, Burlakoti C, Chowdhary CL, Bussmann RW. Indigenous use and ethnopharmacology of medicinal plants in Far-west Nepal. J Plants People Appl Res. 2009;7:005-028.
  8. Rao JM, Srinivas PV, Yadav JS, Raghavan KV, Saxena AK, Shanmugavel M, Kampasi H, Qazi GN. Herbal chemical composition for the treatment of cancer. US Patent. 2003;6:649-650.
  9. Shankaranarayan R, Krishnappa S, Bisarya SC, Dev S. Studies in sesquiterpenes-LIII: Deodarone and atlantolone, new sesquiterpenoids from the wood of Cedrus deodara Loud. Tetrahedron. 1977;33:1201-1205. https://doi.org/10.1016/0040-4020(77)80415-8
  10. Singh SK, Shanmugavel M, Kampasi H, Singh R, Mondhe DM, Rao JM, Adwankar MK, Saxena AK, Qazi GN. Chemically standardized isolates from Cedrus deodara stem wood having anticancer activity. Planta Med. 2007;73:519-526. https://doi.org/10.1055/s-2007-967185
  11. Shinde UA, Phadke AS, Nair AM, Mungantiwar AA, Dikshit VJ, Saraf MN. Membrane stabilizing activity - a possible mechanism of action for the anti inflammatory activity of Cedrus deodara wood oil. Fitoterapia. 1999;70:251-257. https://doi.org/10.1016/S0367-326X(99)00030-1
  12. Wagner H, Bladt S, Rickl V. Plant drug analysis: a thin layer chromatography atlas. (Germany: Springer), 1996.

피인용 문헌

  1. 1. Ethnobotany and phytopharmacology of Pinus roxburghii Sargent: a plant review vol.11, pp.6, 2013, doi:10.5667/tang.2012.0036