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Anti-Metastatic Activity of Juniperus chinensis Extract by Galectin-3 Inhibition

향나무 추출물의 Galectin-3 저해에 의한 암 전이 억제 효과

  • Lee, Keyong Ho (Chong Kun Dang Research Institute, CKD Pharmaceuticals Inc.) ;
  • Rhee, Ki-Hyeong (College of Industrial Sciences, Kongju National University)
  • Received : 2012.08.22
  • Accepted : 2012.10.05
  • Published : 2012.12.31

Abstract

The purpose of this study was to examine the anti-metastatic effect of Juniperus chinensis extract by inhibition of galectin-3 expression. For this study, we carried out the experiment of galectin-3 expression assay and in vivo metastasis mouse model bearing colon 26-M3.1 lung carcinoma, and also MMP-1, MMP-2 and MMP-9 inhibitory assays relating metastasis mechanism. J. chinensis extract significantly showed the inhibitory effect of basal expression and MNNG-induced expression of galectin-3 in colon 26-M3.1 lung carcinoma at the all tested doses (5, 50 and $500{\mu}g/m{\ell}$). In case of the inhibition of MMP-1, MMP-2 and MMP-9, the weak activity was showed at the doses of 50 and $500{\mu}g/m{\ell}$ and was not detected at the dose of $5{\mu}g/m{\ell}$. However, J. chinensis extract significantly showed the anti-metastatic activity on mouse mode bearing colon 26-M3.1 lung carcinoma. Therefore, this study strongly suggests that J. chinensis is a potent inhibitor of galectin-3 expression, and holds great promise for use in inhibiting metastasis induced by elevated expression of galectin-3.

Keywords

Juniperus chinensis;galectin-3;MMPs

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