Healthy Functional Food Properties of Phenolic Compounds Isolated from Ulmus pumila

유근피(Ulmus pumila)로부터 분리한 phenol성 물질의 건강기능식품 활성

  • Kim, Kyung-Bum (School of Food science, Kyungpook National University) ;
  • Jo, Bun-Sung (School of Food science, Kyungpook National University) ;
  • Park, Hye-Jin (School of Applied Bioscience, Kyungpook National University) ;
  • Park, Ki-Tae (Dept. of Hotel Culinary Arts, Dongju University College) ;
  • An, Bong-Jeun (Department of Cosmeceutical, Daegu Hanny University) ;
  • Ahn, Dong-Hyun (Dept. of Food Science and Technology/Institute of Food Science, Pukyong National University) ;
  • Kim, Myung-Uk (Gyeongbuk Institute for Marine Bio-Industry) ;
  • Chae, Jung-Woo (Gyeonggi-Do Forest Environment Research Institute) ;
  • Cho, Young-Je (School of Food science & Biotechnology/Food & Bio-Industry Research Institute, Kyungpook National University)
  • 김경범 (경북대학교 식품과학부) ;
  • 조분성 (경북대학교 식품과학부) ;
  • 박혜진 (경북대학교 응용생명과학부) ;
  • 박기태 (동주대학 호텔조리과) ;
  • 안봉전 (대구한의대학교 화장품약리학과) ;
  • 안동현 (부경대학교 식품가공학과) ;
  • 김명욱 (한국해양바이오연구원) ;
  • 채정우 (경기도산림환경연구소) ;
  • 조영제 (경북대학교 식품공학부/식품생물산업연구소)
  • Received : 2012.07.20
  • Accepted : 2012.11.23
  • Published : 2012.12.30


The phenolic compounds which were extracted with 70% ethanol from Ulmus pumila for 12 hr were the highest as $17.9{\pm}1.0\;mg/g$. DPPH scavenging activity of 70% ethanol extracts was also the highest as $89.5{\pm}1.9%$ and it was confirmed to be high as 80% over in both of water and 70% ethanol extracts containing $50{\mu}g/mL$ over phenolic concentration. ABTS radical cation decolorization activities of water and 70% ethanol extracts were higher as $96.8{\pm}2.9%$, antioxidant protection factor (PF) was 2.0 PF in 70% ethanol and showed higher activities in both of water and 70% ethanol extracts containing $200{\mu}g/mL$ phenolic concentration as 2.5 PF than BHA. TBARs of 70% ethanol extracts was $86.5{\pm}4.6%$, it showed high anti-oxidative activity in $50{\sim}200{\mu}g/mL$ phenolic concentrations of water and 70% ethanol extracts as 80% over. The angiotensin converting enzyme (ACE) inhibitory activity of Ulmus pumila extracts against hypertension was 77.4% and 90.6% in water and 70% ethanol extracts of $200{\mu}g/mL$ phenolic concentration. Xanthine oxidase inhibitory activity of Ulmus pumila extracts for anti-gout effect was not observed in water extracts, but it showed 30% inhibitory activity in 70% ethanol extracts, and 48.1% at $200{\mu}g/mL$ phenolics concentration.


  1. Kim JP, Chin IJ, Cho HK, Ham IH, Whang WK (2004) The antioxidant and the antidiabetic effects of ethanol extract from biofunctional foods prescriptions. Korean J Pharmacogn, 35, 98-103
  2. Aruoma OI (1998) Free radical, oxidative stress and antioxidants in human health and disease. J Am Oil Chem Soc, 75, 199-212
  3. Branen AL (1975) Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. J Am Oil Chem Soc, 52, 59-63
  4. Choe SY, Yang KH (1982) Toxicological studies of antioxidants, butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA). Korean J Food Sci Technol, 12, 283-288
  5. Azuma K, Nakayama M, Koshika M, Ippoushi K, Yamaguchi Y, Kohata K, Yamauchi Y, Ito H, Higashio H (1999) Phenolic antioxidants from the leaves of Corchorus olitorius L. J Agric Food Chem, 47, 3963-3966
  6. Kim SR, Park KM (2003) Physiological activities of Phellinus riis extracrs. Korean J Food Sci Technol, 35, 690-695
  7. Chi HJ, Lee SI (1988) Korea pharmacopoeia Herb medicine standard (Herb medicine). Korea medi indekseuse, p 295
  8. Duke, J. A. 1985. Handbook of medicinal herbs, CRC press, Boca Raton, p.495
  9. Kim CM, Shin MG, Ann DK, Lee KS (1997) The encyclopedia of oriental herbal medicine. Publication Jungdam, p 3348-3350
  10. Folin O, Denis W(1912) On phosphotungastic-phosphomolybdic compounds as color reagents. J Biol Chem, 12, 239-249
  11. Blois MS (1958) Antioxidant determination by the use of a stable free radical. Nature, 26, 1199-1200
  12. Pellegrin N, Roberta R, Min Y, Catherine RE (1998) Screening of diatry carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2'-Azinobis (3-ethylenebenzothiazoline-6-sulfonic acid) radical cation decolorization assay. Method in Enzymology, 299, 379-389
  13. Andarwulan N, Shetty K (1999) Phenolic content in differentiated tissue cultures of untansformed and Ahrobacterium-transformed roots of anise (Pimpinella anisum L). J Agric Food Chem, 47, 1776-1780
  14. Buege JA, Aust SD (1978) Microsomal lipid peroxidation. Method in enzymol, 105, 302-310
  15. Cushman DW, Cheung HS (1971) Spectrophotometic assay and properties of the angiotensin-converting enzyme of rabbit lung. Biochem Pharmacol, 20, 1637-1648
  16. Stirpe F, Corte ED (1969) The regulation of rat liver xanthin oxidase. J Biol Chem, 244, 3855-3861
  17. Gavidson PH, Parish ME (1989) Methods for testing the efficacy of food antimicrobials. J Food Technol, 43, 148-154
  18. Stevenson TH, Lucia LM, Acuff GR (2000) Development of selective medium for isolation of Helicobacter pylori from cattle and beef samples. Appl Environ Micobiol, 66, 723-727
  19. Choi SI, Lee YM, Heo TR (2003) Screening of hyaluronidase inhibitory and free radical scavenging activity in vitro of traditional herbal medicine extracts. Korean J Biotech Bioeng, 18, 282-288
  20. Choi HS, Kim MG, Shin JJ, Pack JM, Lee JS (2003) The antioxidant activities of the some commercial teas. J Korean Soc Food Sci Nutr, 32, 723-727
  21. Shin HL (2003) Biological acivity of phenol compound from muberry fruits. Sangju National University, Food Engineering, MS Thesis
  22. Aoshima H, Tsumoue H, Koda H, Kiso Y (2004) Aging of whiskey increases 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity. J Agric Food Chem, 52, 5240-5244
  23. Miyoshi D, Richard LS (1975) Pulmonary angiotensin converting enzyme. J Biol Chem, 250, 6762-6768
  24. Funayama S, Hikono H (1979) Hypotensive principles of Diospyors kaki Leaves. Chem Pharm Bull, 27, 2865-2871
  25. Stewart JM, Ferreira SH, Greene LJ (1971) Bradykinin poeniating peptide Pca-Lys-Trp-Ala-Pro. An inhibitors of the pulmonary in activation of bradykinin and convertion of angiotensin I to II. Biochem Pharmacol, 20, 157-161
  26. Noro T, Fukushima S (1988) Inhibitors of xanthine oxidase from the flowers and buds of Daphne genkwa, Chem Pharm Bull, 31, 3984-3988
  27. Goodwin CS, Armstrong JA, Chilvers T, Peters M, Collins MD, Sly L, MacConnell W, Harper WES (1989) Transfer of Campylobacter pylori and Campylobacter mustelae to Helicobacter gen. nov. as Helicobacter pylori comb. nov. and Helicobacter mustelue comb. nov. respectively. Int J Syst Bacteriol, 39, 397-402
  28. Konturek SJ, Konturek PC, Pieniazek P, Bielanski W (1999) Role of Helicobacter pylori infection in extragastroduodenal disorders: introductions remarks. J Physiol Pharmacol, 50, 94-683
  29. Ghosh P (1994) The role of hyaluronic acid (hyaluronan) in health and disease : interactions with cells, cartilage and components of synovial fluid. Clin Exp Rheumatol, 12, 75-82

Cited by

  1. Functional food activities of extracts from Pinus densiflora root vol.23, pp.1, 2016,
  2. Effect of Extraction Conditions on in vitro Antioxidant Activities of Root Bark Extract from Ulmus pumila L. vol.44, pp.8, 2015,
  3. Biological Activities of Extracts fromCornus kousaFruit vol.58, pp.4, 2015,
  4. Ulmus macrocarpa Hance Extracts Attenuated H2O2 and UVB-Induced Skin Photo-Aging by Activating Antioxidant Enzymes and Inhibiting MAPK Pathways vol.18, pp.6, 2017,
  5. Effect of extract temperature and duration on antioxidant activity and sensory characteristics of Ulmus pumila bark extract vol.23, pp.7, 2016,
  6. Biological activities of Aster scaber extracts vol.23, pp.3, 2016,
  7. Inhibition of nitric oxide production in LPS-stimulated RAW 264.7 cells by stem bark of Ulmus pumila L. vol.21, pp.5, 2014,