Comparison of Melanogenesis-Inhibiting Activity by Extracts of Prunus persica Flower and Calyx

도화 및 도화악 추출물의 미백활성 비교

  • Son, Hyeong-U (Department of Food Science & Technology, Kyungpook National University) ;
  • Lee, Suk Hee (Cheongdo Peach Experiment Station) ;
  • Kim, Min-A (Department of Food Science & Technology, Kyungpook National University) ;
  • Park, Hee-Joon (CA Pharm Co, Ltd.) ;
  • Lee, Sang-Han (Department of Food Science & Technology, Kyungpook National University)
  • 손형우 (경북대학교 식품공학부) ;
  • 이숙희 (경북농업기술원 청도복숭아시험장) ;
  • 김민아 (경북대학교 식품공학부) ;
  • 박희준 ((주)씨에이팜) ;
  • 이상한 (경북대학교 식품공학부)
  • Received : 2012.09.06
  • Accepted : 2012.12.05
  • Published : 2012.12.30


The antioxidant activity and whitening effect of the distilled water (DW) and ethanol extracts of the Prunus persica flower and calyx were studied. In the oxygen radical absorption capacity (ORAC) assay for antioxidant activity measurement, it was confirmed that the flower extract was stronger than the calyx extract, and that the ethanol extract was relatively stronger than the DW extract. To define the whitening effect, an experiment was conducted involving tyrosinase inhibitory assay and measurement of the melanin content of B16F10. As a result of the use of tyrosinase, the DW extract of calyx showed 53% inhibition as the highest activity. The melanin content inhibitory rates were defined as 57% for the ethanol flower extract and 63% of the ethanol calyx extract, based on a $10{\mu}g/mL$ concentration. Based on these results, mixture with the whitening effect in the extract of P. persica and another compounds should be researched for development as a cosmetic ingredient.


  1. Website of Korea Food & Drug Administration,
  2. Yamaguchi Y, Brenner M, Hearing VJ (2007) The regulation of skin pigmentation. J Biol Chem, 282, 27557-27561
  3. Park HJ, Park KK, Hwang JK, Chung WY, Lee SK (2011) Inhibitory effect of Prunus persica flesh extract (PPFE) on melanogenesis through the microphthalmia-associated transcription factor (MITF)-mediated pathway. Natural Product Sci, 17, 26-32
  4. Olivares C, Solano F (2009) New insights into the active site structure and catalytic mechanism of tyrosinase and its related proteins. Pigment Cell Res, 22, 750-760
  5. Rodriguez-Lopez JN, Tudela J, Varon R,i Garcia-Carmona F, Garcia-Canovas F (1992) Analysis of a kinetic model for melanin biosynthesis pathway. J Biol Chem, 267, 3801-3810
  6. Imokawa G, Mishima Y (1982) Loss of melanogenic properties in tyrosinases induced by glucosylation inhibitors within malignant melanoma cells. Cancer Res, 42, 1994-2002
  7. Gholamhoseinian A, Razmi Z (2012) Screening the methanolic extracts of some plants for tyrosinase inhibitory activity. Toxicol Environ Chem, 94, 310-318
  8. Park JD, Hong SI, Park HW, Kim DM (1999) Modified atmosphere packaging of peaches (Prunus persica L. Batsh) for distribution at ambient temperature. Korean J Food Sci Technol, 31, 1227-1234
  9. Kim KH, Kim DM, Yu S, Yook HS (2012) Antioxidant and whitening activities of various cultivars of Korean unripe peaches (Prunus persica L. Batsch). J Korean Soc Food Sci Nutr, 41, 156-160
  10. Park YJ, Park YS, Jang HG, Heo BG (2005) Utilization of pruning branch of peach tree as a natural dyeing material. Korean J Plant Res, 18, 216-222
  11. Suh SJ, Koo BS, Jin UH, Hwang MJ, Lee IS, Kim CH (2006) Pharmacological characterization of orally active cholinesterase inhibitory activity of Prunus persica L. Batsch in rats. J Mol Neurosci, 29, 101-107
  12. Lee CK, Park KK, Hwang JK, Lee SK, Chung WY (2009) Extract of Prunus persica flesh (PPFE) improves chemotherapeutic efficacy and protects against nephrotoxicity in cisplatin-treated mice. Phytother Res, 22, 223-227
  13. Pastorello EA, Farioli L, Pravettoni V, Ortolani C, Ispano M, Monza M, Baroglio C, Scibola E, Ansaloni R, Incorvaia C, Conti A (1999) The major allergen of peach (Prunus persica) is a lipid transfer protein. J Allergy Clin Immunol, 103, 520-526
  14. Shin TY, Park SB, Yoo JS, Kim IK, Lee HS, Kwon TK, Kim MK, Kim JC, Kim SH (2010) Anti-allergic inflammatory activity of the fruit of Prunus persica: role of calcium and NF-kappaB. Food Chem Toxicol, 48, 2797-2802
  15. Kim DM, Kim KH, Kim YS, Koh JH, Lee KH, Yook HS (2012) A study on the development of cosmetic materials using unripe peaches seed extracts. J Korean Soc Food Sci Nutr, 41, 110-115
  16. Matsuda H, Nakamura S, Kubo M (1994) Studies of cuticle drugs from natural sources. II. Inhibitory effects of Prunus plants on melanin biosynthesis. Biol Pharm Bull, 17, 1417-1420
  17. Benzie IF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": FRAP assay. Anal Biochem, 230, 70-79
  18. Zulueta A, Esteve MJ, Frigola A (2009) ORAC and TEAC assays comparison to measure the antioxidant capacity of food products. Food Chem, 114, 310-316
  19. Yang YJ, Kim HJ, Kang SH, Kang SC (2011) Screening of natural herb resources for anti-oxidative effects in Korea. Korean J Plant Res, 24, 1-9
  20. Ye Y, Chou GX, Mu DD, Wang H, Chu JH, Leung AK, Fong WF, Yu ZL (2010) Screening of Chinese herbal medicines for antityrosinase activity in a cell free system and B16 cells. J Ethnopharmacol, 129, 387-390
  21. Yu J, Kim AK (2009) Effect of taurine on antioxidant enzyme system in B16F10 melanoma cells. Adv Exp Med Biol, 643, 491-499
  22. Yoon NY, Eom TK, Kim MM, Kim SK (2009) Inhibitory effect of phlorotannins isolated from Ecklonia cava on mushroom tyrosinase activity and melanin formation in mouse B16F10 melanoma cells. J Agric Food Chem, 57, 4124-4129
  23. Kim YH, Yang HE, Kim JH, Heo MY, Kim HP (2000) Protection of the flowers of Prunus persica extract from ultraviolet B-induced damage of normal human keratinocytes. Arch Pharm Res, 23, 396-400

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