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Analysis and Uncertainty Estimation of Zearalenone in Cereal-Based Products by LC-MS/MS

LC-MS/MS를 이용한 곡류가공품의 제랄레논 분석과 측정불확도 추정

  • Choi, Eun Jung (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kang, Sung Tae (Department of Food Science and Technology, Seoul National University of Science and Technology) ;
  • Jung, So Young (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Shin, Jae Min (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Jang, Min Su (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Lee, Sang Me (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Jung Hun (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Chae, Young Zoo (Seoul Metropolitan Government Research Institute of Public Health and Environment)
  • 최은정 (서울시보건환경연구원) ;
  • 강성태 (서울과학기술대학교 식품공학과) ;
  • 정소영 (서울시보건환경연구원) ;
  • 신재민 (서울시보건환경연구원) ;
  • 장민수 (서울시보건환경연구원) ;
  • 이상미 (서울시보건환경연구원) ;
  • 김정헌 (서울시보건환경연구원) ;
  • 채영주 (서울시보건환경연구원)
  • Received : 2012.06.21
  • Accepted : 2012.10.15
  • Published : 2012.12.31

Abstract

A survey of zearalenone contamination was conducted on cereal-based products by using an immunoaffinity column with LC-MS/MS. The calibration curve showed good lineality, with correlation coefficients ($R^2$) of 0.999 in the concentration range from 1 to 250 ng/mL. The limits of detection and quantification were approximately $0.3{\mu}g/kg$ and $1.0{\mu}g/kg$, respectively. The recoveries in the barley tea, Misutgaru and snack ranged from 73.6-107.8%. Zearalenone was detected in 10 samples (11.2% incidence). The highest zearalenone contamination level was $29.7{\mu}g/kg$ in the Misutgaru. This survey was conducted with uncertainty of measurement. The expanded uncertainty for zearalenone was estimated to be $44.9{\pm}5.0{\mu}g/kg$ (k=2, 95% confidence level) and $128.7{\pm}7.9{\mu}g/kg$ (k=2, 95% confidence level) for barley tea, $30.7{\pm}5.8{\mu}g/kg$ (k=2, 95% confidence level) and $173.7{\pm}14.9{\mu}g/kg$ (k=2.26, 95% confidence level) for Misutgaru, and $37.2{\pm}7.4{\mu}g/kg$ (k=2.31, 95% confidence level) and $151.0{\pm}10.4{\mu}g/kg$ (k=2, 95% confidence level) snack at the level of $41.7{\mu}g/kg$ and $166.7{\mu}g/kg$, respectively.

Keywords

zearalenone;LC-MS/MS;immunoaffinity column;uncertainty

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