Phenethyl Isothiocyanate Inhibits Ovalbumin-induced Inducible Nitric Oxide Synthase Expression

Ovalbumin에 의해서 유도된 inducible nitric oxide synthase 발현에 대한 phenethyl isothiocyanate의 억제효과

  • Shin, Hwa-Jeong (Department of Medical Science, College of Medical Sciences, Soonchunhyang University) ;
  • Youn, Hyung-Sun (Department of Medical Science, College of Medical Sciences, Soonchunhyang University)
  • 신화정 (순천향대학교 의료과학대학 의료과학과) ;
  • 윤형선 (순천향대학교 의료과학대학 의료과학과)
  • Received : 2012.08.01
  • Accepted : 2012.09.14
  • Published : 2012.12.31


Egg allergies have been reported as one of the most prevalent food hypersensitivities in the pediatric population. One of the major egg allergens is ovalbumin (OVA), which is the major protein in the egg whites. Phenethyl isothiocyanate (PEIC) from cruciferous vegetables has an effect on anti-inflammatory therapy. In the present report, we show that PEIC inhibits the nuclear factor-${\kappa}B$ (NF-${\kappa}B$) activation induced by OVA. PEIC also inhibits the OVA-induced inducible nitric oxide synthase (iNOS) expression and nitrite production. However, PEIC did not suppress the cyclooxygenase-2 (COX-2) expression induced by OVA. These results suggest that PEIC has the specific mechanism for anti-inflammatory responses and efficient anti-allergic activities.


egg allergy;inducible nitric oxide synthase;NF-${\kappa}B$;ovalbumin;phenethyl isothiocyanate


Supported by : 순천향대학교


  1. Youn HS, Saitoh SI, Miyake K, Hwang DH. Inhibition of homodimerization of Toll-like receptor 4 by curcumin. Biochem. Pharmacol. 72: 62-69 (2006)
  2. Youn HS, Lee JY, Fitzgerald KA, Young HA, Akira S, Hwang DH. Specific inhibition of MyD88-independent signaling pathways of TLR3 and TLR4 by resveratrol: Molecular targets are TBK1 and RIP1 in TRIF complex. J. Immunol. 175: 3339-3346 (2005)
  3. Surh YJ. Cancer chemoprevention with dietary phytochemicals. Nat. Rev. Cancer 3: 768-780 (2003)
  4. Zingarelli B, Sheehan M, Wong HR. Nuclear factor-${\kappa}$B as a therapeutic target in critical care medicine. Crit. Care Med. 31(1 Suppl): S105-S111 (2003)
  5. Youn HS, Ahn SI, Lee BY. Guggulsterone suppresses the activation of transcription factor IRF3 induced by TLR3 or TLR4 agonists. Int. Immunopharmacol. 9: 108-112 (2009)
  6. Zhang Y. Cancer-preventive isothiocyanates: Measurement of human exposure and mechanism of action. Mutat. Res. 555: 173-190 (2004)
  7. Lai KC, Hsu SC, Kuo CL, Ip SW, Yang JS, Hsu YM, Huang HY, Wu SH, Chung JG. Phenethyl isothiocyanate inhibited tumor migration and invasion via suppressing multiple signal transduction pathways in human colon cancer HT29 cells. J. Agric. Food Chem. 58: 11148-11155 (2010)
  8. Rose P, Won YK, Ong CN, Whiteman M. Beta-phenylethyl and 8-methylsulphinyloctyl isothiocyanates, constituents of watercress, suppress LPS induced production of nitric oxide and prostaglandin E2 in RAW 264.7 macrophages. Nitric Oxide.-Biol. Ch. 12: 237-243 (2005)
  9. Wang J, Sampson HA. Food allergy: Recent advances in pathophysiology and treatment. Allergy Asthma Immunol. Res. 1: 19-29 (2009)
  10. Mine Y, Yang M. Recent advances in the understanding of egg allergens: Basic, industrial, and clinical perspectives. J. Agric. Food Chem. 56: 4874-4900 (2008)
  11. Huntington JA, Stein PE. Structure and properties of ovalbumin. J. Chromatogr. B. 756: 189-198 (2001)
  12. Turini ME, DuBois RN. Cyclooxygenase-2: A therapeutic target. Annu. Rev. Med. 53: 35-57 (2002)
  13. Moncada S. Nitric oxide: Discovery and impact on clinical medicine. J. Roy. Soc. Med. 92: 164-169 (1999)
  14. Moncada S, Higgs EA. Endogenous nitric oxide: Physiology, pathology, and clinical relevance. Eur. J. Clin. Invest. 21: 361-374 (1991)
  15. Claria J. Cyclooxygenase-2 biology. Curr. Pharm. Des. 9: 2177-2190 (2003)
  16. Lee AN, Park SJ, Jeong AR, Lee JR, Park HJ, Kim SJ, Min IS, Youn HS. Ovalbumin induces cycloxygenase-2 and inducible nitric oxide synthase expression. Korean J. Food Sci. Technol. 43: 110-113 (2011)
  17. Youn HS, Lee JY, Saitoh SI, Miyake K, Kang KW, Choi YJ, Hwang DH. Suppression of MyD88-and TRIF-dependent signaling pathways of Toll-like receptor by (-)-epigallocatechin-3-gallate, a polyphenol component of green tea. Biochem. Pharmacol. 72: 850-859 (2006)
  18. Youn HS, Lee JY, Saitoh SI, Miyake K, Hwang DH. Auranofin, as an anti-rheumatic gold compound, suppresses LPS-induced homodimerization of TLR4. Biochem. Bioph. Res. Co. 350: 866-871 (2006)
  19. Akira S, Takeda K. Toll-like receptor signalling. Nat. Rev. Immunol. 4: 499-511 (2004)
  20. Bjorkbacka H, Fitzgerald KA, Huet F, Li X, Gregory JA, Lee MA, Ordija CM, Dowley NE, Golenbock DT, Freeman MW. The induction of macrophage gene expression by LPS predominantly utilizes MyD88-independent signaling cascades. Physiol. Genomics 19: 319-330 (2004)
  21. Gao JJ, Filla MB, Fultz MJ, Vogel SN, Russell SW, Murphy WJ. Autocrine/paracrine IFN-alphabeta mediates the lipopolysaccharide-induced activation of transcription factor Stat1${\alpha}$ in mouse macrophages: Pivotal role of Stat1${\alpha}$ in induction of the inducible nitric oxide synthase gene. J. Immunol. 161: 4803-4810 (1998)
  22. Hwang D, Jang BC, Yu G, Boudreau M. Expression of mitogeninducible cyclooxygenase induced by lipopolysaccharide: mediation through both mitogen-activated protein kinase and NF-${\kappa}B$ signaling pathways in macrophages. Biochem. Pharmacol. 54: 87-96 (1997)