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Antioxidant Activity of Different Parts of Lespedeza bicolor and Isolation of Antioxidant Compound

싸리나무(Lespedeza bicolor) 부위별 추출물의 항산화 활성 및 항산화물질 분리

  • Lee, Jae-Hak (Department of Animal Products and Food Science, Kangwon National University) ;
  • Jhoo, Jin-Woo (Department of Animal Products and Food Science, Kangwon National University)
  • 이재학 (강원대학교 동물식품응용과학과) ;
  • 주진우 (강원대학교 동물식품응용과학과)
  • Received : 2012.08.14
  • Accepted : 2012.09.26
  • Published : 2012.12.31

Abstract

In this study, total antioxidant properties of extracts from different parts of Lespedeza bicolor were determined using techniques of measuring 1,1-diphenyl-2-picryl hydrazyl/2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)-radical scavenging activity and total phenolic contents. The total antioxidant activities of leaf, stem and root extracts from various solvents (water, 50, 70, 100% ethanol, and hot-water) indicated that 50 and 70% ethanol extracts have high radical scavenging activities and phenolic contents. A systematic approach was used to determine the total antioxidant activity of different solvent fractions of the Lespedeza bicolor extracts, partitioning with chloroform, ethyl acetate, n-butanol, and water, and the ethyl acetate fraction was found to have the strongest antioxidant activity. Antioxidant assay-guided isolation was carried out to isolate potential antioxidant compounds. The ethyl acetate fraction of the leaf extract was subjected to silica gel, LH-20 and RP-18 column chromatography successively, and afforded compound 1, which was identified as eriodictyol by NMR and MS analysis, after which its antioxidant activity was determined.

Keywords

Lespedeza bicolor;antioxidant activity;radical scavenging activity;flavonoid;eriodictyol

Acknowledgement

Supported by : (유)화전민

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