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Essential Oil of Tridax procumbens L Induces Apoptosis and Suppresses Angiogenesis and Lung Metastasis of the B16F-10 Cell Line in C57BL/6 Mice

  • Manjamalai, A. (Department of Biotechnology, Karunya University) ;
  • Kumar, M.J. Mahesh (Centre for Cellular and Molecular Biology) ;
  • Grace, V.M. Berlin (Department of Biotechnology, Karunya University)
  • Published : 2012.11.30

Abstract

Background: To determine the effect of essential oil obtained from a traditionally used medicinal plant Tridax procumbens L, on lung metastasis developed by B16F-10 melanoma cells in C57BL/6 mice. Materials and Methods: Parameters studied were toxicity, lung tumor nodule count, histopathological features, tumor directed capillary vessel formation, apoptosis and expression levels of $P^{53}$ and caspase-3 proteins. Results: In vitro the MTT assay showed cytotoxicity was found to be high as 70.2% of cancer cell death within 24hrs for $50{\mu}g$. In vivo oil treatment significantly inhibited tumor nodule formation by 71.7% when compared with untreated mice. Formation of tumor directed new blood vessels was also found to be inhibited to about 39.5%. TUNEL assays also demonstrated a significant increase in the number of apoptotic positive cells after the treatment. $P^{53}$ and caspase-3 expression was also found to be greater in the essential oil treated group than the normal and cancer group. Conclusions: The present investigation showed significant effects of the essential oil of Tridax procumbens L in preventing lung metastasis by B16F-10 cell line in C57BL/6 mice. Its specific preventive effect on tumor directed angiogenesis and inducing effect on apoptosis warrant further studies at the molecular level to validate the significance of Tridax procumbens L for anticancer therapy.

References

  1. Ahmed A, Lidia AG, Hajer El Jani, et al (2011). Antioxidant and antitumor activities of artemisia campestris and thymelaea hirsuta from Southern Tunisia. Food Chem Toxicol, 49, 342-7. https://doi.org/10.1016/j.fct.2010.11.003
  2. Ait Mbarek L, Ait Mouse HN, Elabbadi N, et al (2007). Antitumor properties of black seed (Nigella sativa L) extracts. Brazilian J Med Biol Res, 40, 839-47. https://doi.org/10.1590/S0100-879X2006005000108
  3. Alisi CS, Emejulu AA, Alisi PNC, et al (2008). Decreased cardiovascular risk and resistance to hyperlipemia-induced hepatic damage in rats by aqueous extract of Urtica dioica. Afr J Biochem Res, 2, 102-6.
  4. Bhagwat DA, Killedar SG, Adnaik SG, et al (2008). Antidiabetic activity of leaf extract of Tridax procumbens L. Int J Green Pharm, 2, 126-8. https://doi.org/10.4103/0973-8258.41188
  5. Bharat B, Aggarwal, Haruyo I, et al (2006). From traditional Ayurvedic medicine to modern medicine: identification of therapeutic targets for suppression of inflammation and cancer. Expert Opin Ther Targets, 10, 87-118. https://doi.org/10.1517/14728222.10.1.87
  6. Chendil D, Ranga RS, Meigooni, D, et al (2004). Sathishkumar S, Ahmed MM. Curcumin confers radiosensitizing effect in prostate cancer cell line PC-3. Oncogene, 23, 1599-607. https://doi.org/10.1038/sj.onc.1207284
  7. Chiang AC, Massague J (2008). Molecular basis of metastasis. N Engl J Med, 359, 2814-23. https://doi.org/10.1056/NEJMra0805239
  8. Cragg GM, Boyd MR (1994). Ethno botany and the search for new drugs. Ciba Foundation Symposium, 185, 178-96.
  9. Devi PU (1996). Withania somnifera Dunal (Ashwagandha): potential plant source of a promising drug for cancer chemotherapy and radiosensitization. Indian J Exp Biol, 34, 927-32.
  10. Eichhorn ME, Kleespies A, Angele MK, et al (2007). Angiogenesis in cancer: molecular mechanisms, clinical impact. Langenbecks Archives of Sur, 392, 371-9. https://doi.org/10.1007/s00423-007-0150-0
  11. Erazo S, Delporte C, Negrete R, et al (2006). Constituents and biological activities of Schinus polygamus. J Ethnopharmacol, 107, 395-400. https://doi.org/10.1016/j.jep.2006.03.028
  12. Fernando SF, Guimaraes, Lucas FA, et al (2010). In vitro and in vivo anticancer properties of a Calcarea carbonica derivative complex (M8) treatment in a murine melanoma model. BMC Cancer, 10, 1-14. https://doi.org/10.1186/1471-2407-10-1
  13. Folkman J, Klagsbrun M (1987). Angiogenic factors. Sci, 235, 442-7. https://doi.org/10.1126/science.2432664
  14. Glucksmann A (1951). Cell deaths in normal vertebrate ontogeny. Biol Rev Camb Philos Soc, 26, 59-86. https://doi.org/10.1111/j.1469-185X.1951.tb00774.x
  15. Gopal K, Kishor KR, Nandini R, et al (2010). High fat diet containing cholesterol induce aortic aneurysm through recruitment and proliferation of circulating agranulocytes in apoE knockout mice model. J Thromb Thrombolysis, 30, 154-63. https://doi.org/10.1007/s11239-010-0446-8
  16. Gopal K, Nagarajan P, Raj TA, et al (2011). Effect of dietary b carotene on cerebral aneurysm and subarachnoid hemorrhage in the brain apo E2/2 mice. J Thromb Thrombolysis, 32, 343-55. https://doi.org/10.1007/s11239-011-0620-7
  17. Guruvayoorappan C, Girija K (2007). Immunomodulatory and antitumor activity of biophytum sensitivum extract. Asian Pac J Cancer Prev, 8, 27-32.
  18. Guy P, Kamatou, Vermaak, et al (2012). Eugenol-from the remote maluku islands to the international market place: a review of a remarkable and versatile molecule. Molecules, 17, 6953-81. https://doi.org/10.3390/molecules17066953
  19. Habila JD, Bello IA, Dzikwi AA, et al (2010). Total phenolics and antioxidant activity of Tridax procumbens L. Afr J Pharm Pharmacol, 4, 123-6.
  20. Ikewuchi CJ, Ikewuchi CC, Onwuka CF, et al (2010). Effect of aqueous extract of Tridax Procumbens Linn on plasma electrolytes of salt-loaded rats. Pak J Nutr, 9, 103-5. https://doi.org/10.3923/pjn.2010.103.105
  21. Kitanaka C, Kuchino Y (1996). Caspase-independent programmed cell death with necrotic morphology. Cell Death Diff, 6, 508-15.
  22. Klein CA (2007). Cancer-the metastasis cascade. Sci, 321, 1785-7.
  23. Konig A, Schwartz GK, Mohammad RM, et al (1997). The novel cyclin-dependent kinase inhibitor flavopiridol downregulates Bcl-2 and induces growth arrest and apoptosis in chronic B-cell leukemia lines. Blood, 90, 4307-12.
  24. Korangath C, Preethi (2010). Inhibition of Metastasis of B16F-10 Melanoma Cells in C57BL/6 Mice by an Extract of Calendula Officinalis L Flowers. Asian Pac J Cancer Prev, 11, 1773-9.
  25. Mahato RB, Chaudhary RP (2005). Ethnomedicinal study and antibacterial activities of selected plants of Palpa district. Nepal Sci World, 3, 26-31.
  26. Mahesh Kumar MJ, Ponvijay KS, Nandhini, et al (2007). A mouse model for Luminal epithelial like ER positive subtype of human breast cancer. BMC Cancer, 7, 1-12. https://doi.org/10.1186/1471-2407-7-1
  27. Manjamalai A, Sardar SS, Guruvayoorappan C, et al (2010). Analysis of phytochemical constituents and anti-microbial activity of some medicinal plants in Tamil Nadu, India. Global J Biotech and Biochem, 5, 120-8.
  28. Mercier B, Prost J, Prost M, et al (2008). Antioxidant activity of bol d'air jacquier breathing sessions in wistar rats-first studies. Int J Occup Environ Hlth Res, 21, 31-46.
  29. Mohammad Shoeb (2006). Anticancer agents from medicinal plants. Bangladesh J Pharmacol, 1, 35-41.
  30. Mosmann T (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunolo Methods, 65, 55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  31. Mraz M, Malinova K, Kotaskova J, et al (2009). MiR-34a, miR-29c and miR-17-5p are down regulated in CLL patients with TP53 abnormalities. Leukemia, 23, 1159-63. https://doi.org/10.1038/leu.2008.377
  32. Nia R, Paper DH, Essien EE, et al (2003). Investigation into in vitro radical scavenging and in vivo anti-inflammatory potential of Tridax procumbens L. Niger J Physiol Sci, 18, 39-43.
  33. Oren M (1994). Relationship of P53 to the control of apoptotic cell death. Seminars in Cancer Biology, 5, 221-7.
  34. Pan Y, Song QL, Lin YH, et al (2005). GLB prevents tumor metastasis of Lewis lung carcinoma by inhibiting tumor adhesion actions. Acta Pharm Sin, 26, 881-6. https://doi.org/10.1111/j.1745-7254.2005.00125.x
  35. Pezzuto JM (1997). Plant-derived anticancer agents. Biochem Pharmacol, 53, 121-33. https://doi.org/10.1016/S0006-2952(96)00654-5
  36. Philip JM, Robert S, Charles C, et al (2001). Gas chromatographic technologies for the analysis of essential oils. J Chromatogr, 936, 1-22. https://doi.org/10.1016/S0021-9673(01)01314-0
  37. Porter AG, Janicke RU (1999). Emerging roles of caspase-3 in apoptosis. Cell Death & Differ, 6, 99-104. https://doi.org/10.1038/sj.cdd.4400476
  38. Raff MC (1992). Social controls on cell survival and cell death. Nature, 356, 397-400. https://doi.org/10.1038/356397a0
  39. Raina R, Prawez S (2008). Medicinal plants and their role in wound healing. Vetscan, 3, 221-4.
  40. Ramirez-Tortosa C, Andersen OM (2001). Anthocyanin rich extract decreases indices of lipid peroxidation and DNA damage in vitamin E-depleted rats. Free Radical Biol Med, 31, 1033-7. https://doi.org/10.1016/S0891-5849(01)00618-9
  41. Richard AD, Georgina SB, Yamina Hamma-Kourbali, et al (2007). Identification of candidate angiogenic inhibitors processed by matrix metalloproteinase 2 (MMP-2) in cellbased proteomic screens: disruption of vascular endothelial growth factor (VEGF)/heparin affin regulatory peptide (pleiotrophin) and VEGF/connective tissue growth factor angiogenic inhibitory complexes by MMP-2 proteolysis. Mol and Cell Biol, 27, 8454-65. https://doi.org/10.1128/MCB.00821-07
  42. Satish A, Bhalerao, Tushar SK, et al (2012). Phytochemical and pharmacological potential of Tridax Procumbens L inn. Int J Adv Biol Res, 2, 392-5.
  43. Setzer WN, Setzer MC, Moriarity DM, et al (1999). Biological activity of the essential oil of myrcianthes sp. nov. "Black fruit" from Monteverde, Costa Rica. Planta Med, 65, 468-79. https://doi.org/10.1055/s-2006-960816
  44. Shan B, Medina JC, Santha E, et al (1999). Selective, covalent modification of beta-tubulin residue Cys-239 by T138067, an antitumor agent with in vivo efficacy against multidrugresistant tumors. Proceedings of the Natl Acad Sci, 96, 5686-91. https://doi.org/10.1073/pnas.96.10.5686
  45. Sheeja K, Kuttan G (2010). Andrographis paniculata down regulates Proinflammatory Cytokine Production and Augments Cell Mediated Immune Response in Metastatic Tumor-Bearing Mice. Asian Pac J Cancer Prev, 11, 723-9.
  46. Takada Y, Murakami A, Aggarwal BB, et al (2005). Zerumbone abolishes NF-kappa B and IkappaBalpha kinase activation leading to suppression of anti-apoptotic and metastatic gene expression, up regulation of apoptosis and down regulation of invasion. Oncogene, 24, 6957-69. https://doi.org/10.1038/sj.onc.1208845
  47. Thornberry NA, Lazebnik Y (1998). Caspases: enemies within. Sci, 281, 1312-6. https://doi.org/10.1126/science.281.5381.1312
  48. Vilwanthan R, Shivshangari KS, Devaki T, et al (2005). Hepatoprotective activity of Tridax procumbens L against d-galactosamine/lipopolysaccharide-induced hepatitis in rats. J Ethnopharmacol, 101, 55-60. https://doi.org/10.1016/j.jep.2005.03.019
  49. Vishnu priya P, Radhika K, Siva kumar R, et al (2011). Evaluation of Anti-cancer activity of Tridax procumbens flower extracts on PC3 Cell Lines. Int J Advan Pharma Sci, 2, 28-30.
  50. Wenfeng Huang, Kun Zou (2011). Cytotoxicity of a plant steroidal saponin on human lung cancer cells. Asian Pac J Cancer Prev, 12, 513-7.
  51. Wong Yau Hsiung, Habsah Abdul Kadir (2011). Leea indica Ethyl Acetate Fraction Induces Growth-Inhibitory Effect in Various Cancer Cell Lines and Apoptosis in Ca Ski Human Cervical Epidermoid Carcinoma Cells. J Evid Based Complementary Altern Med, 11, 1-13. https://doi.org/10.1186/1472-6882-11-1
  52. Wyllie AH, Kerr JFR, Currie AR, et al (1980). Cell death: the significance of apoptosis. Int Rev Cytol, 68, 251-306. https://doi.org/10.1016/S0074-7696(08)62312-8
  53. Xiyun Y, Yun L, Dongling Y, et al (2003). A novel anti-CD146 monoclonal antibody, AA98, inhibits angiogenesis and tumor growth. Blood, 102, 184-91. https://doi.org/10.1182/blood-2002-04-1004
  54. Yang Zhou, Wenyuan Gao, Kefeng Li (2008). Chinese herbal medicine in the treatment of lung cancer. Asian J Trad Med, 3, 1-11.
  55. Zhi Yao, Xu-Chun Che, Rong Lu, et al (2007). Inhibition by Tyroserleutide (Ysl) on the invasion and adhesion of the mouse melanoma cell. Mol Med, 13, 14-21.
  56. Zhou JY, Tang FD, Mao GG, et al (2004). Effect of alpha-pinene on nuclear translocation of NF-kappa B in THP-1 cells. Acta Pharmacol Sin, 25, 480-1.
  57. Zhou JY, Tang FD, Mao GG, et al (2005). Effect of ${\alpha}$-pinene on nuclear translocation of NF-kappa B in THP-1 cells. Acta Pharmacol Sin, 25, 480-4.