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Effect of Triacsin C on LPS-induced Inflammation in 3T3-L1 Adipocytes

LPS에 의해 유도된 3T3-L1 지방세포의 염증반응에 대한 Triacsin C의 효과

  • Park, Eun-Ju (Dept. of Food & Nutrition, Kyungnam University) ;
  • Spurlock, Michael (Dept. of Food Science and Human Nutrition, Iowa State University)
  • Received : 2012.01.26
  • Accepted : 2012.02.07
  • Published : 2012.02.29

Abstract

Triacsin C, an inhibitor of acyl-CoA synthetase, is known to have antiatherosclerotic and vasodilatory activities. The aims of this study were to evaluate the effects of triacsin C on endotoxin-induced (lipopolysaccharide, LPS) inflammation in 3T3-L1 adipocytes and also to evaluate its synergistic effect with triacsin C and resveratrol, a potent antiinflammatory agent. Exposure to LPS for 18 hr increased secretion of IL-6 into the culture medium and mRNA expression of IL-6, MCP-1, TLR and iNOS. Pretreatment of triacsin C for 2 hr suppressed IL-6 accumulation in the medium and the induction of IL-6 expression by LPS, which was more effective than resveratrol treatment. The synergistic effect of triacsin C and resveratrol was found to reduce the expression of iNOS by LPS. However, neither triacsin C nor resveratrol affected the LPS-induced expression of MCP-1, TLR or iNOS. These findings indicate that triacsin C may be a local regulator of inflammation in the adipocyte, although detailed mechanisms are needed to elucidate this through further research.

Acknowledgement

Supported by : 경남대학교

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