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Extract of Listeria monocytogenes Induces the Apoptosis on the Human Promyelocytic Leukemia Cells, HL-60 Cells

Listeria monocytogenes에 의해 HL-60 cell의 세포고사 유도 효과 규명

  • 양은주 (대구한의대학교 보건치료대학 임상병리학과) ;
  • 김동현 (부산가톨릭대학교 보건과학대학 방사선학과) ;
  • 장정현 (대구한의대학교 보건치료대학 임상병리학과)
  • Received : 2011.10.28
  • Accepted : 2011.11.24
  • Published : 2012.02.28

Abstract

Acute promyelocytic leukemia (APL) is a cancer of the blood and bone marrow. Although all-trans retionic acid (ATRA) is the agents for ALP therapy, there are various side effects. For overcome this problem, we need the development of new therapeutic agents for APL. A number of bacteria produce various virulence factors with cytotoxic effects on human cancer cells. To understand the anti-cancer effect of Listeria monocytogenes on APL, we examined alteration of the cell viability, apoptosis and cell cycle arrest of the human promyelocytic leukemia cell line, HL-60 cells. The cell supernatant (LmSup) and the extract of L. monocytogenes (LmE) inhibited the cell viability and induced apoptosis of HL-60 cells. These cytotoxic effect of LmSup and LmE mediated by modulation of cell cycle and ROS production. These results indicate that released or included bacterial molecules from L. monocytogenes have a cytotoxicity in HL-60 cells. Therefore, LmSup and LmE may be used as the potential target for the treatment of cancer induced by HL-60 cells.

Keywords

Listeria monocytogenes;HL-60 cells;Apoptosis;ROS;Anti-cancer Effect

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