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Determination of the PDE-5 Inhibitors and Their Analogues by GC-MS and TMS Derivatization

  • Pyo, Jae-Sung (Southern District Office of National Forensic Service) ;
  • Lee, Hee-Sang (Southern District Office of National Forensic Service) ;
  • Park, Yu-Jin (Southern District Office of National Forensic Service) ;
  • Jo, Ji-Yeong (Southern District Office of National Forensic Service) ;
  • Park, Yong-Hoon (Southern District Office of National Forensic Service) ;
  • Choe, Sang-Gil (Southern District Office of National Forensic Service) ;
  • Lee, Mi-Young (Industrial Safety and Health Research Institute) ;
  • Lee, Jae-Sin (Narcotics Analysis Division of National Forensic Service)
  • Received : 2011.05.02
  • Accepted : 2012.02.23
  • Published : 2012.03.15

Abstract

Eighteen of the PDE-5 inhibitors and their analogues were analyzed using GC-EI-MS. Fourteen of them could be identified by simple GC-MS method without derivatization, but hydroxyhongdenafil, hydroxyvardenafil, xanthoanthrafil and mirodenafil could not be identified without derivatization for the high polarity due to the presence of hydroxyl groups. N,O-bis(trimethylsilyl) trifluoroacetamide (BSTFA) and N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide (MTBSTFA), widely used trimethylsilyl (TMS) derivatizing reagents, were used to improve the sensitivity of the hydroxylated analogues. And the analytes could be identified by GC-MS after the derivatization.

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