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Growth Performance and Meat Quality of Broiler Chickens Supplemented with Bacillus licheniformis in Drinking Water

  • Liu, Xiaolu (Department of Biological Science and Engineering, School of Chemical and Biological Engineering, University of Science and Technology Beijing) ;
  • Yan, Hai (Department of Biological Science and Engineering, School of Chemical and Biological Engineering, University of Science and Technology Beijing) ;
  • Lv, Le (Department of Biological Science and Engineering, School of Chemical and Biological Engineering, University of Science and Technology Beijing) ;
  • Xu, Qianqian (Department of Biological Science and Engineering, School of Chemical and Biological Engineering, University of Science and Technology Beijing) ;
  • Yin, Chunhua (Department of Biological Science and Engineering, School of Chemical and Biological Engineering, University of Science and Technology Beijing) ;
  • Zhang, Keyi (Department of Biological Science and Engineering, School of Chemical and Biological Engineering, University of Science and Technology Beijing) ;
  • Wang, Pei (Department of Biological Science and Engineering, School of Chemical and Biological Engineering, University of Science and Technology Beijing) ;
  • Hu, Jiye (Department of Biological Science and Engineering, School of Chemical and Biological Engineering, University of Science and Technology Beijing)
  • Received : 2011.09.16
  • Accepted : 2012.01.24
  • Published : 2012.05.01

Abstract

A feeding trial was conducted to investigate effects of Bacillus licheniformis on growth performance and meat quality of broilers. Nine hundred one-d-old broiler chicks were randomly assigned to 3 experimental groups with three replicate pens of 100 broiler chicks. Three treatments were i) control, ii) basal diets supplemented with 1 ml of B. licheniformis for each in feed water per day iii) basal diets supplemented with 2 ml of B. licheniformis per chick in feed water per day. The supplementation of B. licheniformis significantly increased body weight in grower chickens (p<0.05), and significantly improved the feed conversion in 3 to 6 and 0 to 6 wk feeding period compared with the control group (p<0.05). Additionally, the supplement also resulted in increased protein and free amino acid contents, and decreased fat content in chicken breast fillet (p<0.05). Furthermore, improvement in sensory attributes was observed in broilers fed with the probiotic. In conclusion, B. licheniformis treatments resulted in a significant increase (p<0.05) in broiler productivity based on an index taking into account daily weight gain and feed conversion rate. Meanwhile, the probiotic contributed towards an improvement of the chemical, nutritional and sensorial characteristics of breast fillet. Overall, the study indicates that B. licheniformis can be used as a growth promoter and meat quality enhancer in broiler poultry.

References

  1. Alexopoulos, C., I. E. Georgoulakis, A. Tzivara, C. S. Kyriakis, A. Govaris and S. C. Kyriakis. 2004. Field evaluation of the effect of a probiotic-containing Bacillus licheniformis and Bacillus subtilis spores on the health status, performance, and carcass quality of grower and finisher pigs. J. Vet. Med. 51:306-312. https://doi.org/10.1111/j.1439-0442.2004.00637.x
  2. AOAC. 1990. Official methods of analysis (15th revised ed.). Washington, DC: Association of Official Analytical Chemists.
  3. Awad, W. A., K. Ghareeb, S. Abdel-Raheem and J. Böhm. 2009. Effects of dietary inclusion of probiotic and synbiotic on growth performance, organ weights, and intestinal histomorphology of broiler chickens. Poult. Sci. 88:49-56 https://doi.org/10.3382/ps.2008-00244
  4. Cavazzoni, V., A. Adami and C. Cstrivilli. 1998. Performance of broiler chickens supplemented with Bacillus coagulans as probiotic. Br. Poult. Sci. 39:526-529. https://doi.org/10.1080/00071669888719
  5. Dikeman, M. E. 1994. Genetics of meat quality. In: Proc. 5th World Congress on Genetics applied to livestock production, 437-449.
  6. Fuller, R. 1989. Probiotics in man and animals. J. Appl. Bacteriol. 66:365-378. https://doi.org/10.1111/j.1365-2672.1989.tb05105.x
  7. Jin, L. Z., Y. W. Ho, N. Abdullah and S. Jalaludin. 1998. Growth performance, intestinal microbial populations, and serum cholesterol of broilers fed diets containing Lactobacillus cultures. Poult. Sci. 77:1259-1265. https://doi.org/10.1093/ps/77.9.1259
  8. Kabir, S. M. L., M. M. Rahman, M. B. Rahman, M. M. Rahman and S. U. Ahmed. 2004. The dynamics of probiotics on growth performance and immune response in broilers. Int. J. Poult. Sci. 3:361-364. https://doi.org/10.3923/ijps.2004.361.364
  9. Kalavathy, R., N. Abdullah, S. Jalaludin and Y. W. Ho. 2003. Effects of Lactobacillus cultures on growth performance, abdominal fat deposition, serum lipids and weight of organs of broiler chickens. Br. Poult. Sci. 44:139-144. https://doi.org/10.1080/0007166031000085445
  10. Karimi Torshizi, M. A., A. R. Moghaddam, S. Rahimi and N. Mojgani. 2010. Assessing the effect of administering probiotics in water or as a feed supplement on broiler performance and immune response. Br. Poult. Sci. 51:178-184. https://doi.org/10.1080/00071661003753756
  11. Kelly, D., R. Begbie and T. P. King. 1994. Nutritional influences on interactions between bacteria and the small intestinal mucosa. Nutr. Res. Rev. 7:233-257. https://doi.org/10.1079/NRR19940013
  12. Knap, I., B. Lund, A. B. Kehlet, C. Hofacre and G. Mathis. 2010. Bacillus licheniformis prevents necrotic enteritis in broiler chickens. Avian Dis. Jun. 54: 931-935. https://doi.org/10.1637/9106-101509-ResNote.1
  13. Kyriakis, S. C., V. K. Tsiloyiannis, J. Vlemmas, K. Sarris, A. C. Tsinas, C. Alexopoulos and L. Jansegers. 1999. The effect of probiotic LSP 122 on the control of post-weaning diarrhoea syndrome of piglets. Res. Vet. Sci. 67:223-228. https://doi.org/10.1053/rvsc.1999.0308
  14. Larbier, M. and G. Uzu. 1991. Control of carcass fatness and quality in broilers: nutritional aspects. Proceedings: 8th European Symposium on Poultry Nutrition, Venezia-Mestre, Italy, 14-17, October 1991. pp. 198-209.
  15. Line, J. E., J. S. Bailey, N. A. Cox, N. J. Stern and T. Tompkins. 1998. Effect of yeast-supplemented feed on Salmonella and Campylobacter populations in broilers. Poult. Sci. 77:405-410. https://doi.org/10.1093/ps/77.3.405
  16. Liu, J. R., S. F. Lai and B. Yu. 2007. Evaluation of an intestinal Lactobacillus reuteri strain expressing rumen fungal xylanase as a probiotic for broiler chickens fed on a wheat-based diet. Br. Poult. Sci. 48:507-514. https://doi.org/10.1080/00071660701485034
  17. Maybray, C. J. and P. J. Waldroup. 1981. The influence of dietary energy and amino acid levels on abdominal fat pad development of the broiler chicken1. Poult. Sci. 60:151-159. https://doi.org/10.3382/ps.0600151
  18. Mohan, B., R. Kadirvel, A. Natarajan and M. Bhaskaran. 1996. Effect of probiotic supplementation on growth, nitrogen utilization and serum cholesterol in broilers. Br. Poult. Sci. 37:395-401. https://doi.org/10.1080/00071669608417870
  19. Mountzouris, K. C., P. Tsirtsikos, E. Kalamara, S. Nitsch, G. Schatzmayr and K. Fegeros. 2007. Evaluation of the efficacy of a probiotic containing lactobacillus, bifidobacterium, enterococcus, and pediococcus strains in promoting broiler performance and modulating cecal microflora composition and metabolic activities. Poult. Sci. 86:309-317. https://doi.org/10.1093/ps/86.2.309
  20. Nahashon, S. N., H. S. Nakaue, S. P. Snyder and L. W. Mirosh. 1994. Performance of single comb white leghorn layers fed corn-soybean meal and barley-corn-soybean meal diets supplemented with a direct-fed microbial. Poult. Sci. 73:1712-1723. https://doi.org/10.3382/ps.0731712
  21. Nardonea, A. and F. Valfre. 1999. Effects of changing production methods on quality of meat, milk and eggs. Livest. Prod. Sci. 59:165-182. https://doi.org/10.1016/S0301-6226(99)00025-1
  22. Osipova, I. G., I. B. Sorokulova, E. A. Vasil'eva and E. V. Budanova. 2005. Pre-clinical trials of new spore probiotics. Vestn. Ross. Akad. Med. Nauk 36-40.
  23. Owings, W. J., D. L. Reynolds, R. J. Hasiak and P. R. Ferket. 1990. Influence of dietary supplementation with Streptococcus faecium M-74 on broiler body weight, feed conversion, carcass characteristics, and intestinal microbial colonization. Poult. Sci. 69:1257-1264. https://doi.org/10.3382/ps.0691257
  24. Pascual, M., M. Hugas, J. I. Badiola, J. M. Monfort and M. Garriga. 1999. Lactobacillus salivarius CTC2197 prevents Salmonella enteritidis colonization in chickens. Appl. Environ. Microbiol. 65:4981-4986.
  25. Patterson, J. A. and K. M. Burkholder. 2003. Application of prebiotics and probiotics in poultry production. Poult. Sci. 82:627-631. https://doi.org/10.1093/ps/82.4.627
  26. Rada, V. and I. Rychly. 1995. The effect of Lactobacillus salivarius administration on coliform bacteria and enterococci in the crop and cecum of broiler chickens. Vet. Med. (Praha) 40:311-315.
  27. Salminen, S., C. Bouley, M. C. Boutron-Ruault, J. H. Cummings, A. Franck, G. R. Gibson, E. Isolauri, M. C. Moreau, M. Roberfroid and I. Rowland. 1998. Functional food science and gastrointestinal physiology and function. Br. J. Nutr. 80 (Suppl. 1):S147-171. https://doi.org/10.1079/BJN19980108
  28. Samanya, M. and K. E. Yamauchi. 2002. Histological alterations of intestinal villi in chickens fed dried Bacillus subtilis var. natto. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 133:95-104. https://doi.org/10.1016/S1095-6433(02)00121-6
  29. SCAN. 2000b. Report of the scientific committee on animal nutrition on product BioPlus 2B for use as feed additive. European commission, health and consumer protection directorate-general. (SCAN) Scientific Committee on Animal Nutrition.
  30. Sogaard, D. H. and T. Suhr-Jessen. 1990. Microbials for feed beyond lactic acid bacteria. Feed International. 11:32-38.
  31. Cutting, S. M. 2011. Bacillus probiotics. Food Microbiol. 28:214-220. https://doi.org/10.1016/j.fm.2010.03.007
  32. Smith, E. R. and G. M. Pesti. 1998. Influence of broiler strain cross and dietary protein on the performance of broilers. Poult. Sci. 77:276-281. https://doi.org/10.1093/ps/77.2.276
  33. Spackman, D. H., W. H. Stein and S. Moore. 1958. Automatic recording apparatus for use in the chromatography of amino acids. Anal. Chem. 30:1190-1206. https://doi.org/10.1021/ac60139a006
  34. SPSS, 2010. SPSS Version 19.0.0 for Windows. SPSS Inc., USA.
  35. Steel, R. G. D. and J. H. Torrie. 1980. Principles and procedures of statistics. A Biometrical Approach. McGraw-Hill, New York, NY.
  36. Tannock, G. W. 1999. What we know and need to know. Biotechnol. Adv. 17:691-693. https://doi.org/10.1016/S0734-9750(99)00021-X
  37. Timmerman, H. M., A. Veldman, E. van den Elsen, F. M. Rombouts and A. C. Beynen. 2006. Mortality and growth performance of broilers given drinking water supplemented with chicken-specific probiotics. Poult. Sci. 85:1383-1388. https://doi.org/10.1093/ps/85.8.1383
  38. Tomasik, P. J. and P. Tomasik. 2003. Probiotics and prebiotics. Cereal Chem. 80:113-117. https://doi.org/10.1094/CCHEM.2003.80.2.113
  39. Tortuero, F. 1973. Influence of the implantation of Lactobacillus acidophilus in chicks on the growth, feed conversion, malabsorption of fats syndrome and intestinal flora. Poult. Sci. 52:197-203. https://doi.org/10.3382/ps.0520197
  40. Zulkifli, I., N. Abdullah, N. M. Azrin and Y. W. Ho. 2000. Growth performance and immune response of two commercial broiler strains fed diets containing Lactobacillus cultures and oxytetracycline under heat stress conditions. Br. Poult. Sci. 41:593-597. https://doi.org/10.1080/713654979

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