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Sex Determination of Cattle Meat by Polymerase Chain Reaction Amplification of the DEAD Box Protein (DDX3X/DDX3Y) Gene

  • Gokulakrishnan, P. (Division of Livestock Products Technology, Indian Veterinary Research Institute) ;
  • Kumar, R.R. (Division of Livestock Products Technology, Indian Veterinary Research Institute) ;
  • Sharma, B.D. (Division of Livestock Products Technology, Indian Veterinary Research Institute) ;
  • Mendiratta, S.K. (Division of Livestock Products Technology, Indian Veterinary Research Institute) ;
  • Sharma, D. (Genome Mapping Laboratory, Central Avian Research Institute)
  • Received : 2012.01.01
  • Accepted : 2012.03.17
  • Published : 2012.05.01

Abstract

Determination of sex origin of cattle meat by fast and reliable molecular methods is an important measure to ensure correct allocation of export refunds particularly in European countries and also female cattle (cow) slaughter is legally banned in India because of religious beliefs. Based on the DEAD box protein gene located on the X and Y chromosomes, 2 pair of primers were designed and the system of PCR was optimized. Upon PCR amplification, male tissue showed 2 bands, while female tissue resulted in only one band. The accuracy and specificity of the primers was assessed using DNA template extracted from cattle meat of known sex. The protocol was subjected to a blind test and showed 100% concordance, proving its accuracy and reliability.

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Cited by

  1. Determination of Sex Origin of Meat and Meat Products on the DNA Basis: A Review vol.55, pp.10, 2015, https://doi.org/10.1080/10408398.2012.690095