DOI QR코드

DOI QR Code

Effect of Peripheral Administration of Kisspeptin-10 on Dynamic LH Secretion in Prepubertal Ewes

  • Wang, Jun ;
  • Sun, Lei ;
  • Zhang, Tao ;
  • Zhou, Haizhu ;
  • Lou, Yujie
  • Received : 2011.10.25
  • Accepted : 2012.02.05
  • Published : 2012.06.01

Abstract

The aim of the present study was to clarify the effect of kisspeptin-10 on LH secretion in prepubertal ewes. In experiment 1, prepubertal ewes fitted with indwelling jugular catheters were randomly assigned to receive 0, 0.5, 1 or 2 mg of kisspeptin-10 dissolved in saline, and serial blood samples were collected at 15-min intervals for 180 min to analyze the response curves of LH after injection. In experiment 2, prepubertal ewes fitted with indwelling jugular catheters were injected with 0 or 1 mg of kisspeptin-10 dissolved in saline and the injection was repeated 3 times at 1 h interval and serial blood samples were collected at 15-min intervals for 210 min to analyze the response curves of LH after injection. The results showed that single intravenous administration of 0.5, 1 and 2 mg of kisspeptin-10 all could significantly increased LH secretion in prepubertal ewes, and the effect of 1 and 2 mg of kisspeptin-10 on LH secretion was higher than that of 0.5 mg group. The results also showed that repeated intravenous administration of kisspeptin-10 could effectively increase LH secretion and repeated administration did not influence the effect of kisspeptin-10 on LH secretion in prepubertal ewe. In conclusion, the present study indicated that single or repeated intravenous administration of kisspeptin-10 could effectively increase LH secretion in prepubertal ewes.

Keywords

Kisspeptin-10;LH;Prepubertal Ewes

References

  1. Arreguin-Arevalo, J. A., C. A. Lents, T. A. Farmerie, T. M. Nett, and C. M. Clay. 2007. KiSS-1 peptide induces release of LH by a direct effect on the hypothalamus of ovariectomized ewes. Anim. Reprod. Sci. 101:265-275. https://doi.org/10.1016/j.anireprosci.2006.09.021
  2. Castellano, J., V. Navarro, R. Fernandez-Fernandez, J. Castano, M. Malagon, E. Aguilar, C. Dieguez, P. Magni, L. Pinilla and M. Tena-Sempere. 2006. Ontogeny and mechanisms of action for the stimulatory effect of kisspeptin on gonadotropin-releasing hormone system of the rat. Mol. Cell. Endocrinol. 257:75-83. https://doi.org/10.1016/j.mce.2006.07.002
  3. De Roux, N., E. Genin, J. Carel, F. Matsuda, J. Chaussain and E. Milgrom. 2003. Hypogonadotropic hypogonadism due to loss of function of the KiSS1-derived peptide receptor GPR54. Proceedings of the National Academy of Sciences of the United States of America. Proc. Natl. Acad. Sci USA. 100:10972-10976. https://doi.org/10.1073/pnas.1834399100
  4. Funes, S., J. Hedrick, G. Vassileva, L. Markowitz, S. Abbondanzo, A. Golovko, S. Yang, F. Monsma and E. Gustafson. 2003. The KiSS-1 receptor GPR54 is essential for the development of the murine reproductive system. Biochem. Biophys. Res. Commun. 312:1357-1363. https://doi.org/10.1016/j.bbrc.2003.11.066
  5. Han, S. K., M. L. Gottsch, K. J. Lee, S. M. Popa, J. T. Smith, S. K. Jakawich, D. K. Clifton, R. A. Steiner and A. E. Herbison. 2005. Activation of gonadotropin-releasing hormone neurons by kisspeptin as a neuroendocrine switch for the onset of puberty. J. Neurosci. 25:11349-11356. https://doi.org/10.1523/JNEUROSCI.3328-05.2005
  6. Hashizume, T., H. Saito, T. Sawada, T. Yaegashi, A. A. Ezzat, K. Sawai and T. Yamashita. 2010. Characteristics of stimulation of gonadotropin secretion by kisspeptin-10 in female goats. Anim. Reprod. Sci. 118:37-41. https://doi.org/10.1016/j.anireprosci.2009.05.017
  7. Ohtaki, T., Y. Shintani, S. Honda, H. Matsumoto, A. Hori, K. Kanehashi, Y. Terao, S. Kumano, Y. Takatsu and Y. Masuda. 2001. Metastasis suppressor gene KiSS-1 encodes peptide ligand of a G-protein-coupled receptor. Nature 411:613-617. https://doi.org/10.1038/35079135
  8. Ojeda, S., V. Prevot, S. Heger, A. Lomniczi, B. Dziedzic and A. Mungenast. 2003. Glia-to-neuron signaling and the neuroendocrine control of female puberty. Ann. Med. 35:244-255. https://doi.org/10.1080/07853890310005164
  9. Plant, T. M. and S. Ramaswamy. 2009. Kisspeptin and the regulation of the hypothalamic-pituitary-gonadal axis in the rhesus monkey (Macaca mulatta). Peptides 30:67-75. https://doi.org/10.1016/j.peptides.2008.06.029
  10. Pompolo, S., A. Pereira, K. Estrada and I. Clarke. 2006. Colocalization of kisspeptin and gonadotropin-releasing hormone in the ovine brain. Endocrinology 147:804-810. https://doi.org/10.1210/en.2005-1123
  11. Roa, J., E. Aguilar, C. Dieguez, L. Pinilla and M. Tena-Sempere. 2008. New frontiers in kisspeptin/GPR54 physiology as fundamental gatekeepers of reproductive function. Front. Neuroendocrinol. 29:48-69. https://doi.org/10.1016/j.yfrne.2007.07.002
  12. Tena-Sempere, M. 2006. GPR54 and kisspeptin in reproduction. Hum. Reprod. Update 12:631-639. https://doi.org/10.1093/humupd/dml023
  13. Terasawa, E. 2005. Role of GABA in the mechanism of the onset of puberty in non-human primates. Int. Rev. Neurobiol. 71:113-129. https://doi.org/10.1016/S0074-7742(05)71005-9
  14. Todman, M., S. Han and A. Herbison. 2005. Profiling neurotransmitter receptor expression in mouse gonadotropin-releasing hormone neurons using green fluorescent protein-promoter transgenics and microarrays. Neuroscience 132:703-712. https://doi.org/10.1016/j.neuroscience.2005.01.035
  15. Tovar, S., M. J. Vázquez, V. M. Navarro, R. Fernández-Fernández, J. M. Castellano, E. Vigo, J. Roa, E. F. Casanueva, E. Aguilar, L. Pinilla, C. Dieguez and M. Tena-Sempere. 2006. Effects of single or repeated intravenous administration of kisspeptin upon dynamic LH secretion in conscious male rats. Endocrinology 147:2696-2704. https://doi.org/10.1210/en.2005-1397