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Mutation of Cellulose Synthase Gene Improves the Nutritive Value of Rice Straw

  • Su, Yanjing (College of Animal Science and Technology, Yangzhou University) ;
  • Zhao, Guoqi (College of Animal Science and Technology, Yangzhou University) ;
  • Wei, Zhenwu (College of Animal Science and Technology, Yangzhou University) ;
  • Yan, Changjie (Key Laboratory of Plant Functional Genomics, Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology, Yangzhou University) ;
  • Liu, Sujiao (College of Animal Science and Technology, Yangzhou University)
  • Received : 2011.11.07
  • Accepted : 2012.02.03
  • Published : 2012.06.01

Abstract

Rice straw is an important roughage resource for ruminants in many rice-producing countries. In this study, a rice brittle mutant (BM, mutation in OsCesA4, encoding cellulose synthase) and its wild type (WT) were employed to investigate the effects of a cellulose synthase gene mutation on rice straw morphological fractions, chemical composition, stem histological structure and in situ digestibility. The morphological fractions investigation showed that BM had a higher leaf sheath proportion (43.70% vs 38.21%, p<0.01) and a lower leaf blade proportion (25.21% vs 32.14%, p<0.01) than WT. Chemical composition analysis showed that BM rice straw was significantly (p<0.01) higher in CP (crude protein), hemicellulose and acid insoluble ash (AIA) contents, but lower in dry matter (DM), acid detergent fiber (ADFom) and cellulose contents when compared to WT. No significant difference (p>0.05) was detected in neutral detergent fiber (NDFom) and ADL contents for both strains. Histological structure observation indicated that BM stems had fewer sclerenchyma cells and a thinner sclerenchyma cell wall than WT. The results of in situ digestion showed that BM had higher DM, NDFom, cellulose and hemicellulose disappearance at 24 or 48 h of incubation (p<0.05). The effective digestibility of BM rice straw DM and NDFom was greater than that of WT (31.4% vs 26.7% for DM, 29.1% vs 24.3% for NDFom, p<0.05), but the rate of digestion of the slowly digested fraction of BM rice straw DM and NDF was decreased. These results indicated that the mutation in the cellulose synthase gene could improve the nutritive value of rice straw for ruminants.

Keywords

Cellulose Synthase;Rice Straw;Histological Structure;In situ Digestibility

References

  1. Abou-El-Enin, O. H., J. G. Fadel and D. J. Mackill. 1999. Differences in chemical composition and fibre digestion of rice straw with, and without, anhydrous ammonia from 53 rice varieties. Anim. Feed Sci. Technol. 79:129-136. https://doi.org/10.1016/S0377-8401(98)00271-5
  2. Agbagla-Dohnani, A., P. Noziere, G. Clement and M. Doreau. 2001. In sacco degradability, chemical and morphological composition of 15 varieties of European rice straw. Anim. Feed Sci. Technol. 94:15-27. https://doi.org/10.1016/S0377-8401(01)00296-6
  3. AOAC. 1999. Official methods of analysis. 16th ed. Association of Official Analytical Chemists, Wahington, DC, USA.
  4. Bal, M. A., R. D. Shaver, A. G. Jirovec, K. J. Shinners and J. G. Coors. 2000. Crop processing and chop length of corn silage: Effects on intake, digestion, and milk production by dairy cows. J. Dairy Sci. 83:1264-1273. https://doi.org/10.3168/jds.S0022-0302(00)74993-9
  5. Beauchemin, K. A. and W. Z. Yang. 2005. Effects of physically effective fiber on intake, chewing activity, and ruminal acidosis for dairy cows fed diets based on corn silage. J. Dairy Sci. 88:2117-2129. https://doi.org/10.3168/jds.S0022-0302(05)72888-5
  6. Guo, T. S., M. D. Sánchez and P. Y. Guo. 2002. Animal production based on crop residues-chinese experiences. In: Introduction (Ed. T. S. Guo and Z. H. Yang). FAO, Rome, Italy. pp. 6-8.
  7. Jung, H. G. and M. S. Allen. 1995. Characteristics of plant cell walls affecting intake and digestibility of forages by ruminants. J. Anim. Sci. 73:2774-2790.
  8. Karunanandaa, K., G. A. Varga, D. E. Akin, L. L. Rigsby and D. J. Royse. 1995. Botanical fractions of rice straw colonized by white-rot fungi: Changes in chemical composition and structure. Anim. Feed Sci. Technol. 55:179-199. https://doi.org/10.1016/0377-8401(95)00805-W
  9. Keys, J. E., P. J. Van Soest and E. P. Young. 1969. Comparative study of the digestibility of forage cellulose and hemicellulose in ruminants and nonruminants. J. Anim. Sci. 29:11-15.
  10. Kononoff, P. J., A. J. Heinrichs and H. A. Lehman. 2003. The effect of corn silage particle size on eating behavior, chewing activities, and rumen fermentation in lactating dairy cows. J. Dairy Sci. 86:3343-3353. https://doi.org/10.3168/jds.S0022-0302(03)73937-X
  11. Liu, J. X. and E. R. Orskov. 2000. Cellulase treatment of untreated and steam pre-treated rice straw-effect on in vitro fermentation characteristics. Anim. Feed Sci. Technol. 88:189-200. https://doi.org/10.1016/S0377-8401(00)00218-2
  12. Liu, J. X., A. Susenbeth and K. H. Südekum. 2002. In vitro gas production measurements to evaluate interactions between untreated and chemically treated rice straws, grass hay, and mulberry leaves. J. Anim. Sci. 80:517-524.
  13. McDonald, I. M. 1981. A revised model for the estimation of protein degradability in rumen. J. Agric. Sci. (Camb.) 96:251-252. https://doi.org/10.1017/S0021859600032081
  14. Persson, S., H. Wei, J. Milne, G. P. Page and C. R. Somerville. 2005. Identification of genes required for cellulose synthesis by regression analysis of public microarray data sets. Proc. Natl. Acad. Sci. USA 102:8633-8638. https://doi.org/10.1073/pnas.0503392102
  15. Orskov, E. R. and L. McDonald. 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. J. Agric. Sci. (Camb.) 92:499-503. https://doi.org/10.1017/S0021859600063048
  16. Rahal, A., A. Singh and M. Singh. 1997. Effect of urea treatment and diet composition on, and prediction of nutritive value of rice straw of different cultivars. Anim. Feed Sci. Technol. 68: 165-182. https://doi.org/10.1016/S0377-8401(97)00045-X
  17. Selim, A. S. M., J. Pan, T. Takano, T. Suzuki, S. Koike, Y. Kobayashi and K. Tanaka. 2004. Effect of ammonia treatment on physical strength of rice straw, distribution of straw particles and particle-associated bacteria in sheep rumen. Anim. Feed Sci. Technol. 115:117-128. https://doi.org/10.1016/j.anifeedsci.2004.01.011
  18. Singh, M. and H. P. Singh. 1995. Genetic variation in chemical composition and digestibility of nutrients in rice straw. Int. Rice Res. Notes 20:4.
  19. Sohane, R. K. and M. Singh. 2000. Genetic variability and prediction of nutritive value of rice straw of different cultivars. Indian J. Anim. Nutr. 17:271-278.
  20. Vadiveloo, J. 2000. Nutritional properties of the leaf and stem of rice straw. Anim. Feed Sci. Technol. 83:57-65. https://doi.org/10.1016/S0377-8401(99)00107-8
  21. Vadiveloo, J. and J. G. Fadel. 2009. The response of rice straw varieties to urea treatment. Anim. Feed Sci. Technol. 151:291-298. https://doi.org/10.1016/j.anifeedsci.2009.03.003
  22. Van Soest, P. J., J. B. Robertson and B. A. Lewis. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3583-3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2
  23. Wang, H. F., Y. M. Wu, J. X. Liu and Q. Qian. 2006. Morphological fractions, chemical compositions and in vitro gas production of rice straw from wild and brittle culm1 variety harvested at different growth stages. Anim. Feed Sci. Technol. 129:159-171. https://doi.org/10.1016/j.anifeedsci.2005.12.009
  24. Wang, J. K., X. L. Chen, J. X. Liu, Y. M. Wu and J. A. Ye. 2008. Histological changes of tissues and cell wall of rice straw influenced by chemical pretreatments. Asian-Aust. J. Anim. Sci. 21:824-830. https://doi.org/10.5713/ajas.2008.70487
  25. Wang, J. K., J. X. Liu, J. Y. Li, Y. M. Wu and J. A. Ye. 2007. Histological and rumen degradation changes of rice straw stem epidermis as influenced by chemical pretreatment. Anim. Feed Sci. Technol. 136:51-62. https://doi.org/10.1016/j.anifeedsci.2006.08.017
  26. Weimer, P. J., D. R. Mertens, E. Ponnampalam, B. F. Severin and B. E. Dale. 2003. Fibex-treated rice straw as a feed ingredient for lactating dairy cows. Anim. Feed Sci. Technol. 103:41-50. https://doi.org/10.1016/S0377-8401(02)00282-1
  27. Welch, J. G. 1982. Rumination, particle size and passage from the rumen. J. Anim. Sci. 54:885-894.
  28. Wilman, D., G. R. Foulkes and D. I. Grivens. 1996. A comparison of four methods of estimating the rate and extent of cell wall degradation in grass silages. Anim. Feed Sci. Technol. 63:99-109. https://doi.org/10.1016/S0377-8401(96)01016-4
  29. Yan, C., S. Yan, X. Zeng, Z. Zhang and M. Gu. 2007. Fine mapping and isolation of bc7(t), allelic to OsCesA4. J. Genet. Genomics 34:1019-1027. https://doi.org/10.1016/S1673-8527(07)60115-5

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