Differential Proteome Analysis of Breast and Thigh Muscles between Korean Native Chickens and Commercial Broilers

  • Liu, Xian De ;
  • Jayasena, Dinesh D. ;
  • Jung, Yeon-Kuk ;
  • Jung, Samooel ;
  • Kang, Bo-Seok ;
  • Heo, Kang-Nyeong ;
  • Lee, Jun-Heon ;
  • Jo, Cheo-Run
  • Received : 2011.10.18
  • Accepted : 2012.02.02
  • Published : 2012.06.01


The Korean native chickens (Woorimotdak$^{TM}$, KNC) and commercial broilers (Ross, CB) show obvious differences in meat flavor after cooking. To understand the contribution of protein and peptide for meat flavor, 2-dimensional (2-D) gel electrophoresis and matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF) mass spectrometry was performed. A total of 16 protein spots were differentially expressed in the breast and thigh meat between the two breeds. A total of seven protein spots were represented by different levels between KNC and CB for breast meat. Among them three protein spots (TU39149, TU40162 and TU39598) showed increases in their expressions in KNC while other four protein spots (BU40125, BU40119, BU40029 and BU39904) showed increases in CB. All nine protein spots that were represented by different levels between KNC and CB for thigh meat showed increases in their expression in KNC. Phosphoglucomutase 1 (PGM 1), myosin heavy chain (MyHC), heat shock protein B1 (HSP27), cytochrome c reductase (Enzyme Q), Glyoxylase 1, DNA methyltransferase 3B (DNA MTase 3) were identified as the main protein spots by MALDI-TOF mass spectrometry. These results can provide valuable basic information for understanding the molecular mechanism responsible for breed specific differences in meat quality, especially the meat flavour.


Korean Native Chickens;Broilers;2-D Electrophoresis;MALDI-TOF Mass pectrometry


  1. Ahn, D. H. and S. Y. Park. 2002. Studies on components related to taste such as free amino acids and nucleotides in Korean native chicken meat. J. Korean Soc. Food Sci. Nutr. 31:547-552.
  2. Choe, J. H., K. Nam, S. Jung, B. Kim, H. J. Yun and C. Jo. 2010. Difference in the quality characteristics between Korean native chickens and broilers. Korean J. Food Sci. Anim. Resour. 30: 13-19.
  3. Gao, X., X. Wen, L. Esser, B. Quinn, L. Yu, C. A. Yu and D. Xia. 2003. Structural basis for the quinone reduction in the bc1 complex: a comparative analysis of crystal structures of mitochondrial cytochrome bc1 with bound substrate and inhibitors at the Qi site. Biochemistry 42:9067-9080.
  4. Hamelin, M., T. Sayd, C. Chambon, J. Bouix, B. Bibe, D. Milenkovic, H. Leveziel, M. Georges, A. Clop, P. Marinova and E. Laville. 2006. Proteomic analysis of ovine muscle hypertrophy. J. Anim. Sci. 84:3266-3276.
  5. Hamelin, M., T. Sayd, C. Chambon, J. Bouix, B. Bibe, D. Milenkovic, H. Leveziel, M. Georges, A. Clop, P. Marinova and E. Laville. 2007. Differential expression of sarcoplasmic proteins in four heterogeneous ovine skeletal muscles. Proteomics 7:271-280.
  6. Han, I. K., J. K. Ha and J. H. Lee. 2009. Growth and development of the academic societies and animal production in Korea, China and Asia over the last 50 years. Asian-Aust. J. Anim. Sci. 22:906-914.
  7. Han, R. X., H. R. Kim, K. Naruse, S. M. Choi, B. C. Kim, S. P. Park and D. I. Jin. 2007. Comparative study of protein profile during development of mouse placenta. Reprod. Develop. Biol. 31:253-269.
  8. Hemmer, W., M. Skarli, J. C. Perriard and T. Wallimann. 1993. Effect of okadaic acid on protein phosphorylation patterns of chicken myogenic cells with special reference to creatine kinase. FEBS Lett. 327:35-40.
  9. Hollung, K., H. Grove, E. M. Feargestend, M. S. Sidhu and P. Berg. 2009. Comparison of muscle proteome profile in Norwegian Landrace, Duroc and Hampshire at three different age. Meat Sci. 81:487-492.
  10. Izquierdo, J. M. 2006. Control of the ATP synthase beta subunit expression by rna-binding proteins tia-1, tiar, and hur. Biochem. Biophys. Res. Commun. 348:703-711.
  11. Jaturasitha, S. 2004. Meat management. Mingmuang Press, Chiang Mai. Thailand.
  12. Jaturasitha, S., T. Srikanchai, M. Kreuzer and M. Wicke. 2008. Difference in carcass and meat characteristics between chicken indigenous to northern Thailand (blackboned and Thai native) and imported extensive breeds (Bresse and Rhode Island Red). Poult. Sci. 87:160-169.
  13. Jeon, H. J., J. H. Choe, Y. Jung, Z. A. Kruck, D. G. Lim and C. Jo. 2010. Comparion of the chemical composition, textural characteristics, and sensory properties of north and south Korean negative chicken and commercial broilers. Korean J. Food Sci. Anim. Resour. 30:171-178.
  14. Jung, Y., H. J. Jeon, S. Jung, J. H. Choe, J. H. Lee, K. N. Heo, B. S. Kang and C. Jo. 2011. Comparison of quality traits of thigh meat from Korean native chickens and broilers. Korean J. Food Sci. Anim. Resour. 31:684-692.
  15. Kim, N. K., J. H. Lim, M. J. Song, O. H. Kim, B. Y. Park, M. J. Kim, I. H. Hwang and C. S. Lee. 2007. Developmental proteomic profiling of porcine skeletal muscle during postnatal development. Asian-Aust. J. Anim. Sci. 20:1612-1617.
  16. Kim, N. K., J. H. Lim, M. J. Song, O. H. Kim, B. Y. Park, M. J. Kim, I. H. Hwang and C. S. Lee. 2008. Comparisons of long-issimus muscle metabolic enzymes and muscle fiber types in Korean and western pig breeds. Meat Sci. 78:455-460.
  17. Kong, H. S., J. D. Oh, J. H. Lee, K. J. Jo, B. D. Sang, C. H. Choi, S. D. Kim, S. J. Lee, S. H. Yeon, G. J. Jeon and H. K. Lee. 2006. Genetic variation and relationships of Korean native chickens and foreign breeds using 15 microsatellite markers. Asian-Aust. J. Anim. Sci. 19:1546-1550.
  18. Lefaucheur, L., D. Milan, P. Ecolan and C. Le Callennec. 2004. Myosin heavy chain composition of different skeletal muscles in large white and meishan pigs. J. Anim. Sci. 82:1931-1941.
  19. Sang, B. D., S. K. Hong, H. K. Kim, C. H. Choi, S. D. Kim, Y. M. Cho, B. C. Sang, J. H. Lee, G. J. Jeon and H. K. Lee. 2006. Estimation of genetic parameters for economic traits in Korean native chickens. Asian-Aust. J. Anim. Sci. 19:319-323.
  20. Sentandreu, M. A., M. Armenteros, J. J. Calvete, A. Ouall, M. C. Aristoy and F. Toldra. 2007. Proteomic identification of actin derived oligopeptides in dry cured ham. J. Agric. Food Chem. 55:3613-3619.
  21. Spanier, A. M., M. Flores, K. W. Mcmillin and T. D. Bidner. 1997. The effect of postmortem aging on meat flavor quality. Correlation of treatment, sensory, instrumental, and chemical descriptors. Food Chem. 59:531-538.
  22. Spanier, A. M., M. Flores, F. Toldra, M. C. Aristoy, K. L. Bett, P. Bystricky and J. M. Bland. 2004. Meat flavor: contribution of proteins and peptides to the flavor of beef. Adv. Exp. Med. Biol. 542:33-49.
  23. Sugiyama, Y., A. Suzuki, M. Kishikawa, R. Akutsu, T. Hirose, M. M. Waye, S. K. Tsui, S. Yoshida and S. Ohno. 2000. Muscle develops a specific form of small heat shock protein complex composed of mkbp/hspb2 and hspb3 during myogenic differentiation. J. Biol. Chem. 275:1095-1104.
  24. Wimmers, K., N. T. Ngu, D. G. J. Jennen, D. Tesfaye, E. Murani, K. Schellander and S. Ponsuksili. 2008. Relationship between myosin heavy chain isoform expression and muscling in several diverse pig breeds. J. Anim. Sci. 86:795-803.
  25. Xu, Y. J., M. L. Jin, L. J. Wang, A. D. Zhang, B. Zuo, D. Q. Xu, Z. Q. Ren, M. G. Lei, X. Y. Mo, F. E. Li, R. Zheng, C. Y. Deng and Y. Z. Xiong. 2009. Differential proteomic analysis of porcine skeletal muscle between Meishan and Large White. J. Anim. Sci. 87:2519-2527.
  26. Yoshida, M., S. Minamisawa, M. Shimura, S. Komazaki, H. Kume, M. Zhang, K. Matsumura, M. Nishi, M. Saito, Y. Saeki, Y. Ishikawa, T. Yanagisawa and H. Takeshima. 2005. Impaired $Ca^{2+}$ store functions in skeletal and cardiac muscle cells from sarcalumenin-deficient mice. J. Biol. Chem. 280:3500-3506.

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Grant : Cooperative Research Program for Agriculture Science and Technology Development

Supported by : Rural Development Administration