DOI QR코드

DOI QR Code

Application of ATP Bioluminescence Assay for Measurement of Microbial Contamination in Fresh-cut Produce Processing Lines

신선편이 농식품 생산라인의 환경미생물 오염도 측정을 위한 ATP 검사법의 이용

  • Choi, Ji-Weon (National Institute of Horticultural and Herbal Science, RDA) ;
  • Lee, Hye-Eun (National Institute of Horticultural and Herbal Science, RDA) ;
  • Kim, Chang-Kug (National Academy of Agricultural Science, RDA) ;
  • Kim, Won-Bae (National Institute of Horticultural and Herbal Science, RDA) ;
  • Kim, Ji-Kang (National Institute of Horticultural and Herbal Science, RDA)
  • 최지원 (농촌진흥청 국립원예특작과학원) ;
  • 이혜은 (농촌진흥청 국립원예특작과학원) ;
  • 김창국 (농촌진흥청 국립농업과학원) ;
  • 김원배 (농촌진흥청 국립원예특작과학원) ;
  • 김지강 (농촌진흥청 국립원예특작과학원)
  • Received : 2011.08.01
  • Accepted : 2011.12.16
  • Published : 2012.02.28

Abstract

With the rapid growth of fresh-cut produce market, the South Korean fresh-cut industry is facing the challenge of ensuring food safety. As the estimation of the microbial numbers in fresh-cut produce processing lines (tools, and equipment) using the conventional microbiological techniques takes days, so there is a need for faster and easier monitoring methods. This study was conducted to investigate the use of ATP bioluminescence assay to measure the degree of microbial contamination from three actual fresh-cut processing lines. The samples collected from frech-cut vegetables, and fresh-cut fruits processing plants were tested for the estimation of the bacterial number, using the ATP bioluminescence and microbiological methods. The result of former was transferred to log RLU/100 $cm^2$, and that of the latter was transferred to log CFU/100 $cm^2$. A positive linear correlation between the ATP bioluminescence assay value and aerobic-plate count was found for fresh-cut processing lines, with a correlation coefficient of 0.8772 (n=50). The results of this study indicate that ATP bioluminescence assay can be used to monitor microbial contamination in fresh-cut produce processing plants, and can help improve the hygiene therein.

References

  1. Ukuku DO, Sapers GM, Fett WF (2005) ATP bioluminescence assay for estimation of microbial populations of fresh-cut melon. J Food Prot, 68, 2427-2432 https://doi.org/10.4315/0362-028X-68.11.2427
  2. Griffiths MW (1996) The role of ATP bioluminescence in the food industry: new light on old problems. Food Technol, 50, 63-72
  3. Beuchat LR (1995) Pathogenic microorganisms associated with fresh produce. J Food Prot, 59, 204-216
  4. Ukuku DO, Fett WF (2002) Effectiveness of chlorine and nisin-EDTA treatments of whole melons and fresh-cut pieces for reducing native microflora and extending shelf-life. J Food Saf, 22, 231-253 https://doi.org/10.1111/j.1745-4565.2002.tb00344.x
  5. Kang HM, Eom YS, An HS, Kim CJ, Choi KH, Chung CI (2000) Application of ATP Bioluminescence method for measurement of microbial contamination in raw meat, meat and dairy processing line. J Fd Hyg Safety, 15, 252-255
  6. Kim BY, Song HY, Park IS, Kim YS, Lee YS, Ha SD (2009) A correlation study of surveillance data and ATP bioluminescence assay for verification of hygienic status in major hotels in Seoul. J Fd Hyg Safety, 24, 277-284
  7. Velazquez M, Feirtag JM (1997) Quenching and enhancement effects of ATP extractants, cleansers, and sanitizers on the detection of the ATP bioluminescence signal. J Food Prot, 60, 799-803 https://doi.org/10.4315/0362-028X-60.7.799
  8. Lee YS, Kim SH, Kim DH (2009) Current Status of Fresh-Cut Market in Korea and Stimulus Measures. KREI R-602
  9. KFDA (2007) Reform on standard and specification of food. KFDA Notification No 2007-63
  10. KFDA (2006) Manual of HACCP Application and Management. Korea Food & Drug Administration, Seoul, Korea, p 42-66
  11. Park YH, Seo KS, Ahn JS, Yoo HS, Kim SP (2001) Evaluation of the petrifilm plate method for the enumeration of aerobic microorganism and coliforms in retailed meat samples. J Food Prot, 64, 1841-1843 https://doi.org/10.4315/0362-028X-64.11.1841
  12. Goldschmidt MC, Fung DYC (1979) Automated instrumentation for microbiological analysis. Food Technol, 3, 63-70

Cited by

  1. New biocide for eco-friendly biofilm removal on outdoor stone monuments 2017, https://doi.org/10.1016/j.ibiod.2017.03.004