Application of ATP Bioluminescence Assay for Measurement of Microbial Contamination in Fresh-cut Produce Processing Lines

신선편이 농식품 생산라인의 환경미생물 오염도 측정을 위한 ATP 검사법의 이용

  • Choi, Ji-Weon (National Institute of Horticultural and Herbal Science, RDA) ;
  • Lee, Hye-Eun (National Institute of Horticultural and Herbal Science, RDA) ;
  • Kim, Chang-Kug (National Academy of Agricultural Science, RDA) ;
  • Kim, Won-Bae (National Institute of Horticultural and Herbal Science, RDA) ;
  • Kim, Ji-Kang (National Institute of Horticultural and Herbal Science, RDA)
  • 최지원 (농촌진흥청 국립원예특작과학원) ;
  • 이혜은 (농촌진흥청 국립원예특작과학원) ;
  • 김창국 (농촌진흥청 국립농업과학원) ;
  • 김원배 (농촌진흥청 국립원예특작과학원) ;
  • 김지강 (농촌진흥청 국립원예특작과학원)
  • Received : 2011.08.01
  • Accepted : 2011.12.16
  • Published : 2012.02.28


With the rapid growth of fresh-cut produce market, the South Korean fresh-cut industry is facing the challenge of ensuring food safety. As the estimation of the microbial numbers in fresh-cut produce processing lines (tools, and equipment) using the conventional microbiological techniques takes days, so there is a need for faster and easier monitoring methods. This study was conducted to investigate the use of ATP bioluminescence assay to measure the degree of microbial contamination from three actual fresh-cut processing lines. The samples collected from frech-cut vegetables, and fresh-cut fruits processing plants were tested for the estimation of the bacterial number, using the ATP bioluminescence and microbiological methods. The result of former was transferred to log RLU/100 $cm^2$, and that of the latter was transferred to log CFU/100 $cm^2$. A positive linear correlation between the ATP bioluminescence assay value and aerobic-plate count was found for fresh-cut processing lines, with a correlation coefficient of 0.8772 (n=50). The results of this study indicate that ATP bioluminescence assay can be used to monitor microbial contamination in fresh-cut produce processing plants, and can help improve the hygiene therein.


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