DOI QR코드

DOI QR Code

Immunological Activity of Solvent Fractions from $Epimedium$ $koreanum$ Nakai

삼지구엽초 용매별 분획 추출물의 면역관련 활성

  • Park, Myoung-Su (Department of Food Science and Biotechnology, School of Biotechnology and Bioengineering and Institute of Bioscience & Biotechnology, Kangwon National University) ;
  • Kim, Seo-Jin (Department of Food Science and Biotechnology, School of Biotechnology and Bioengineering and Institute of Bioscience & Biotechnology, Kangwon National University) ;
  • Wang, Jun (Department of Food Science and Biotechnology, School of Biotechnology and Bioengineering and Institute of Bioscience & Biotechnology, Kangwon National University) ;
  • Kim, Gwang-Hee (Department of Food Science and Biotechnology, School of Biotechnology and Bioengineering and Institute of Bioscience & Biotechnology, Kangwon National University) ;
  • Oh, Deog-Hwan (Department of Food Science and Biotechnology, School of Biotechnology and Bioengineering and Institute of Bioscience & Biotechnology, Kangwon National University)
  • 박명수 (강원대학교 바이오산업공학부 식품생명공학과) ;
  • 김서진 (강원대학교 바이오산업공학부 식품생명공학과) ;
  • 왕준 (강원대학교 바이오산업공학부 식품생명공학과) ;
  • 김광희 (강원대학교 바이오산업공학부 식품생명공학과) ;
  • 오덕환 (강원대학교 바이오산업공학부 식품생명공학과)
  • Received : 2011.09.09
  • Accepted : 2012.01.13
  • Published : 2012.02.28

Abstract

$Epimedium$ $koreanum$ Nakai is a wild medicinal plant commonly consumed in South Korea due to its beneficial health effects. In this study, the antimutagenic and immunological activities of $E.$ $koreanum$ Nakai extracts were investigated for their use in food. In the immunomodulating activity, the effects of $E.$ $koreanum$ Nakai on the B cell (Rhamos) and T cell (Molt-4) were investigated. The results showed that the growth and viability of the B and T cells were increased and activated more in the ethylacetate (1.35 and 1.48 times) and water fraction (1.30 and 1.40 times), respectively. In the Ames test, none of the fractions produced a mutagenic effect on $Salmonella$. $typhimurium$ TA98 and TA 100. The ethylacetate fraction showed a strong antimutagenic effect (98%) on and a high butanol fraction (84%) of B(${\alpha}$)P in $S.$ $typhimurium$ TA98 and TA100, respectively. In 4-nitroquinoline-1-oxide (4NQO), all the solvent fractions showed an over 70% antimutagenic effect, except for the chloroform extract. Especially, ethylacetate and butanol showed strong inhibition of the mutagenic effects (80 and 90%) on 4NQO in $S.$ $typhimurium$ TA98 and TA100, respectively. These results provide preliminary data for the development of $E.$ $koreanum$ Nakai as an edible food material.

References

  1. Miyase T, Ueno A, Takizawa N, Kobayashi H, Oguchi H (1987) Studies in the glycosides of Epimedium grandiflorum. Chem Pharm Bull, 35, 3713-3716 https://doi.org/10.1248/cpb.35.3713
  2. Kang SS, Shin KH, Ahn SD, Park KY, Kang CS (1997) Difference in components of Epimedium koreanum. in compliance with seasons and places of ollection. Kor J Med Crop Sci, 4, 321-328
  3. Lee YG, Sohn HO, Lee DW, Lim HB (2002) The effect of water-extract of Epimedium koreanum Nakai on agerelated change of the xenobiotic metabolizing enzyme system in the liver of rats. Kor J Med Crop Sci, 10, 29-36
  4. Noh JH, Kim YJ, Choi KJ, Kim SW, Kim SK, Kim JH (2003) Characteristics of seeding and rhizome propagation in Epimedium koreanum Nakai. Kor J Med Crop Sci, 11, 155-160
  5. Kang CS, Choi BR, Park KY, Ahn SD (1997) Establishment of growth environment and mass propagation system development for Epimedium Koreanum. Kor J Pharmacogn, 30, 378-381
  6. Alley MC, Scudiero DA, Monks A, Hursey ML, Czerwinski MJ, Fine DL, Abbortt BJ, Mayo JG, Shoemarker RH, Boyd MR (1988) Feasibility of drug screening with panels of human tumor cell line using a microculture tetrazolium assay. Cancer Res, 48, 589-601
  7. Carmichael J, De Graff WG, Gazder AF, Minna JD, Mitchell JB (1987) Evaluation of the tetrazolium based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res, 47, 936-942
  8. Ames BN, Maron DM (1983) Revised methods for the Salmonella typhyimurium mutagenicity test. Mutation Res, 113, 173-215 https://doi.org/10.1016/0165-1161(83)90010-9
  9. Kim MJ, Kim JS, Kang WH, Jeong DM (2002) Effect on antimutagenic and cancer cell growth inhibition of Lxeris dentata Nakai. Kor J Med Crop Sci, 10, 139-143
  10. Hwang HS, Ham SS (1999) Antimutagenic and cytotoxic effects of aster acaber root ethanol extract. Kor J Food Sci Technol, 31, 1065-1070
  11. Ahn BY, Lee KS, Maeng IK, Song GS, Choi DS (1998) Bioantimutagenic effects of water extract from Rehmannia glutinosa Liboschitz in SOS chromotest. Kor J Food Sci Technol, 30, 439-445
  12. Park MH, Kang SM, Jung HY, Hong SG (2003) Protecting effects of vitamin E against immobilization stress-induced oxidative damage in rat brain. Kor Nutr, 36, 570-576
  13. Gruter A, Friederich V, Wurgler FE (1990) Antimutagenic effects of mushrooms. Mutation Res, 231, 243-249 https://doi.org/10.1016/0027-5107(90)90030-8
  14. Lai CH, Butler MN, Matney TS (1980) Antimutagenic activities of common vegetable and their chlorophyll content. Mutation Res, 77, 245-250 https://doi.org/10.1016/0165-1218(80)90057-9
  15. Lee JH. (1989) Studies on the demutagenic effect of the edible mountain herb juices. MS Thesis, Kangwon National University
  16. Ito Y, Maeda S, Sugiyama T (1986) Suppression of 7,12-dimethylbenzo(a)anthracene-induced chromosome aberrations in rat bone marrow cells by vegetable juices. Mutation Res, 172, 55-60 https://doi.org/10.1016/0165-1218(86)90106-0
  17. Han KS, Ham SS, Jeong EH, Lee HK (1992) Antimutagenic effects of the edible mountain herb juices against Trp-P-1 and 2-AF. Kor J Food Hyg, 7, 161-168
  18. Kang SS, Kim JS, Kang YJ, Han HK (1990) Studies on the underground parts of Epimedium koreanum. Kor J Pharmacogn, 21, 56-59
  19. Kang SS, Shin KH, Chung SG, Cho EH (1998) Flavonoids from Epimedium koreanum. Kor J Pharmacogn, 19, 93-98
  20. Xu SC, Xu BJ, Wang MT (1987) Isolation and identification of icariin and icariside I. Chin Pharm Bull, 22, 129-132
  21. Xu SC, Wang ZX, Wu LJ, Wang NB, Chen YJ (1982) Isolation and identification do icariin and epimedoside A. Chin Tard Herb Drug, 13, 9-15
  22. Fukai T, Nomura T (1988) Seven prenylated flavonol glycosides from two epimedium species. Phytochem, 27, 259-266 https://doi.org/10.1016/0031-9422(88)80627-7
  23. Mizuno M, Hanioka S, Suzuki N, Iinuma M, Tanaka T, Liu XS, Min ZD (1987) Flavonol glycosides from Epimedium sagittatum. Phytochem, 226, 861-863
  24. Sun P, Zhao Ye W, Pei J, Wang Z, Chen Y, Ogihara Y, Takeda T (1995) Studies on the constituents of Epimedium koreanum. Chem Pharm Bull, 43, 703-704 https://doi.org/10.1248/cpb.43.703
  25. Lee MK, Choi YJ, Sung SH, Shin DI, Kim JW, Kim YC (1995) Antihepatotoxic activity of icariin, a major constituent of Epimedium koreanum. Planta Med, 61, 523-526 https://doi.org/10.1055/s-2006-959362

Cited by

  1. Antioxidative Effects of Extracts from Different Parts of Epimedium koreanum Nakai vol.45, pp.2, 2016, https://doi.org/10.3746/jkfn.2016.45.2.188