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Antioxidant and Anti-Adipogenic Effects of Ethanolic Extracts from Tartary and Common Buckwheats

쓴메밀 및 단메밀 에탄올 추출물의 항산화 및 지방세포 분화억제 효과

  • Yoon, Bo-Ra (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Cho, Bong-Jae (Department of Food Science, Kongju National University) ;
  • Lee, Hyo-Ku (Department of Food Science, Kongju National University) ;
  • Kim, Dae-Jung (Department of Food Science, Chungbuk National University) ;
  • Rhee, Seong-Kap (Department of Food and Biotechnology, Hoseo University) ;
  • Hong, Hee-Do (Korea Food Research Institute) ;
  • Kim, Kyung-Tack (Korea Food Research Institute) ;
  • Cho, Chang-Won (Korea Food Research Institute) ;
  • Choi, Hyeon-Son (Department of Food Science and Biotechnology, CHA University) ;
  • Lee, Boo-Yong (Department of Food Science and Biotechnology, CHA University) ;
  • Lee, Ok-Hwan (Department of Food Science and Biotechnology, Kangwon National University)
  • Received : 2011.07.27
  • Accepted : 2011.12.23
  • Published : 2012.02.28

Abstract

In this study, 80% ethanolic extracts of tartary and common buckwheats were assessed for their total phenol content, total flavonoids content, antioxidant activity (DPPH, ABTS radical scavenging activity and reducing power), and anti-adipogenic effects. Our results show that total phenol contents of 80% ethanolic extract from tartary and common buckwheats were $17.35{\pm}0.41$ and $8.20{\pm}0.28\;{\mu}g$ GAE/g, respectively. Antioxidant activities of 80% ethanolic extract from tartary buckwheat were significantly higher than that of common buckwheat extract (p<0.05). During adipocyte differentiation, 80% ethanolic extracts of tartary and common buckwheat significantly inhibited lipid accumulation compared to control cells. We further evaluated the effect of buckwheat extracts on the changes of key gene expression associated with 3T3-L1 adipogenesis and ROS production. Tartary buckwheat extract was more suppressed the mRNA expressions ($PPAR{\gamma}$ and aP2) than that of common buckwheat extract. Moreover, tartary buckwheat inhibited the mRNA expression of both NOX4 (NADPH oxidase 4) and G6PDH (glucose-6-phosphate dehydrogenase). These results indicate that anti-adipogenesis effect of tartary buckwheat can be attributed to phenolic compound that may potentially inhibit ROS production.

Acknowledgement

Supported by : 강원대학교, 중소기업청

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