Study on Comparison of Ingredient Quantities and Biological Activities of Galgeun-tang according to Extraction Solvent

용매별 갈근탕 추출물의 성분 분석 및 효능 비교

  • Kim, Ye Ji (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Kim, Ohn Soon (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Seo, Chang Seob (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Lim, Hye Sun (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Yoo, Sae Rom (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Jeon, Woo Young (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Jin, Seong Eun (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Shin, In Sik (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Kim, Jung Hoon (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Shin, Na Ra (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Kim, Seong Sil (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Lee, Mee Young (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Jeong, Soo Jin (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Ha, Hye Kyung (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Shin, Hyeun Kyoo (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine)
  • 김예지 (한국한의학연구원 한약기초연구그룹) ;
  • 김온순 (한국한의학연구원 한약기초연구그룹) ;
  • 서창섭 (한국한의학연구원 한약기초연구그룹) ;
  • 임혜선 (한국한의학연구원 한약기초연구그룹) ;
  • 유새롬 (한국한의학연구원 한약기초연구그룹) ;
  • 전우영 (한국한의학연구원 한약기초연구그룹) ;
  • 진성은 (한국한의학연구원 한약기초연구그룹) ;
  • 신인식 (한국한의학연구원 한약기초연구그룹) ;
  • 김정훈 (한국한의학연구원 한약기초연구그룹) ;
  • 신나라 (한국한의학연구원 한약기초연구그룹) ;
  • 김성실 (한국한의학연구원 한약기초연구그룹) ;
  • 이미영 (한국한의학연구원 한약기초연구그룹) ;
  • 정수진 (한국한의학연구원 한약기초연구그룹) ;
  • 하혜경 (한국한의학연구원 한약기초연구그룹) ;
  • 신현규 (한국한의학연구원 한약기초연구그룹)
  • Received : 2012.09.26
  • Accepted : 2012.12.17
  • Published : 2012.12.25

Abstract

To investigate the difference and change of ingredient and efficacy of Galgeun-tang (GGT) according to extraction solvent, water and 70% EtOH, the quantities of index components and the several in vitro activities of two kinds of GGT extract were compared. The contents of extracts were analyzed with HPLC. The biological activities such as anti-inflammatory and anti-obesity effects were measured through cell line-based in vitro assay. The cytotoxicity was measured by CCK-8 assay in RAW 264.7 cell, BEAS-2B cell, HaCaT cell and 3T3-L1 cell. We compared effects of two kinds of extract by measuring NO, PGE2, IL-6 and TNF-${\alpha}$ in RAW264.7 cell, RANTES in BEAS-2B cell, MDC and RANTES in HaCaT cell and GPDH activity and leptin level in differentiated 3T3-L1. 70% EtOH extract of GGT contained more compositions than water extract. The inhibitory effect of water extract of GGT on NO in RAW 264.7 cell and GPDH activity in 3T3-L1 was stronger than that of 70% EtOH. 70% EtOH has a stronger inhibitory effect on PGE2 in RAW264.7 cell, RANTES in BEAS-2B cell, MDC, RANTES in HaCaT cell, leptin in 3T3-L1 cell than water extraction. These results suggest that the ingredient and efficacy from 70% EtOH extract of GGT are more effective than water extract.

Acknowledgement

Supported by : 한국한의학연구원

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