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Antioxidant and Antimicrobial Activities of Ethanol Extract from Six Vegetables Containing Different Sulfur Compounds

황 함유 채소 에탄올 추출물의 항산화 및 항균활성

  • Kim, Kyoung-Hee (Dept. of Food and Nutrition, Chungnam National University) ;
  • Kim, Hye-Joung (Dept. of Food and Nutrition, Chungnam National University) ;
  • Byun, Myung-Woo (Dept. of Culinary Nutrition, Woosong University) ;
  • Yook, Hong-Sun (Dept. of Food and Nutrition, Chungnam National University)
  • 김경희 (충남대학교 식품영양학과) ;
  • 김혜정 (충남대학교 식품영양학과) ;
  • 변명우 (우송대학교 외식조리영양학부) ;
  • 육홍선 (충남대학교 식품영양학과)
  • Received : 2012.01.31
  • Accepted : 2012.02.28
  • Published : 2012.05.31

Abstract

This study investigated the antioxidant activities, and antimicrobial activity $in$ $vitro$ of an 80% ethanol extract from garlic, daikon, leek, ginger, onion, and green onion, which are widely-used ingredients in Korean food that contain sulfur. The total polyphenol content in ginger and leek extracts showed a high value ($233.63{\pm}4.59$ and $220.98{\pm}10.56$ mg/g GAE) and onions, leeks, garlic, and daikon followed by with $69.07{\pm}1.42$, $68.83{\pm}2.11$, $19.41{\pm}0.40$, $19.05{\pm}03.32$ mg/g GAE, respectively. DPPH radical scavenging activity was highest with ginger extracts ($1.57{\pm}0.15$ mg/mL as $IC_{50}$) followed in order of decreasing activity by leeks, onions, daikon, green onions, and garlic. The results of ABTS radical scavenging activity and FRAP value showed higher antioxidant activity in extracts from ginger and leek. The order of vegetables with most to least prevalent ABTS radical scavenging activity was green onions, onions, garlic, and finally daikon. From greatest to least FRAP value, the relevant vegetables were green onions, onions, daikon, and garlic (p<0.05). Ginger extracts showed promise against seven strains of microbes: $Bacillus$ $cereus$, $Bacillus$ $subtillis$, $Staphylococcus$ $aureus$, $Lactobacillus$ $plantarum$, $Escherichia$ $coli$, $Salmonella$ $enterica$, and $Pseudomonas$ $aeruginosa$. Garlic extracts (5 mg/disc) showed strong antimicrobial activity against $B.$ $cereus$ (22.3 mm) and $E.$ $coli$ (24.3 mm). Extracts of both onion and green onion showed antimicrobial activity against only $E.$ $coli$ (12.7 and 10.3 mm) and $B.$ $cereus$ (12.0 and 12.5 mm) at 10 mg/disc, and the inhibition zone diameter from extracts of garlic and leeks were 18.0 mm and 10.4 mm vs. $L.$ $plantarum$ at 10 mg/disc. This study showed positive antioxidant activities for ginger and leeks, and positive antimicrobial activities for leeks and garlic. These sulfur-containing vegetables are widely used in Korean food. Leeks especially could serve as a functional food preservative.

References

  1. Halliwell B, Gutteridge JMC. 1989. Free Radicals in Biology and Medicine. Oxford University, NY, USA. p 398-401.
  2. Farinati F, Cardin R, Degan P, Rugge M, Mario FD, Bonvicini P, Naccarato R. 1998. Oxidative DNA damage accumulation in gastric carcinogenesis. Gut 42: 351-356. https://doi.org/10.1136/gut.42.3.351
  3. Cooked MS, Mistry N, Wood C, Hebert KE, Lunce J. 1997. Immunogenicity of DNA damaged by ROS-implications for anti-DNA antibodies in lupus. Free Radic Biol Med 22: 151-159. https://doi.org/10.1016/S0891-5849(96)00283-3
  4. Darely-Usmer V, Halliwell B. 1996. Blood radicals; reactive nitrogen species, reactive oxygen species, transition metal ions and the vascular system. Pharm Res 13: 649-662. https://doi.org/10.1023/A:1016079012214
  5. Parthasarathy S, Steinberg D, Witztum JL. 1992. The role of oxidezed LDL in the pathogenesis of antherosclerosis. Ann Rev Med 43: 219-225. https://doi.org/10.1146/annurev.me.43.020192.001251
  6. Laurindo FR, Da Luz PL, Uint L, Rocha TF, Jaeger RG, Lopes EA. 1991. Evidence for superoxide radical dependent coronary artery vasospasm after antigioplasry in intact dogs. Circulation 83: 1705-1715. https://doi.org/10.1161/01.CIR.83.5.1705
  7. Nakazono K, Watanabe N, Matsuno K, Sasaki J, Sato T, Inoue M. 1991. Does superoxide underlie the pathogenesis of hypertension? Proc Natl Acad Sci USA 88: 10045-10048. https://doi.org/10.1073/pnas.88.22.10045
  8. Jeong SJ, Lee H, Song NH, Lee SE, Baeg I. 2004. Natural products chemistry: screening for antioxidative activity of plant medicinal extracts. J Korean Soc Food Sci Nutr 33: 28-33. https://doi.org/10.3746/jkfn.2004.33.1.028
  9. Yasmine M. 1997. Global estimation of foodborne disease. World Health Statistics Quarterly 50: 5-11.
  10. Kwun JW, Lee CH. 2007. Trends of recent food-borne disease outbreaks in Korea. J Korean Med Assoc 50: 573-581. https://doi.org/10.5124/jkma.2007.50.7.573
  11. Lee KS, Lee JC, Na SL, Jung HY, Lim KT. 1999. Effects on mammalian tissues and cells by sulfur containing compounds. J Toxicol Pub Health 15: 79-87.
  12. Gillissen S, Nowak D. 1998. Characterization of N-acetylcysteine and ambroxol anti-oxidant therapy. Respir Med 92: 609-614. https://doi.org/10.1016/S0954-6111(98)90506-6
  13. Sainani G, Desai D, Katrodia K, Valame V, Sainani P. 1979. Onion, garlic and experimental atherosclerosis. Jpn Heart J 20: 351-357. https://doi.org/10.1536/ihj.20.351
  14. Bordia A. 1978. Effect of garlic on human platelet aggregation in vitro. Atherosclerosis 30: 355-360. https://doi.org/10.1016/0021-9150(78)90129-6
  15. Subrahmanyan V, Sreenivasamurthy V, Krishnamurthy K, Swaminathan M. 1958. The effect of garlic on certain intestinal bacteria. Food Sci 7: 223-230.
  16. Qiao L, Shiff SJ, Rigas B. 1997. Sulindac sulfide inhibits the proliferation of colon cancer cell: diminished expression of the proliferation markers PCVA and KI-67. Cancer Lett 115: 229-234. https://doi.org/10.1016/S0304-3835(97)04740-X
  17. Fukushima S, Takada N, Hori T, Wamibuchi H. 1997. Cancer preventation by organosulfur compounds from garlic and onion. J Cell Biochem Suppl 27: 100-106.
  18. Folin O, Denis W. 1912. On phosphotungstic-phosphomolybdic compounds as color reagents. J Biol Chem 12: 239-249.
  19. Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1198-1200.
  20. Pellegrin N, Roberta R, Min Y, Catherine RE. 1998. Screening of dietary carotenoids and carotenoid-rich fruit extract for antioxidant activities applying 2,2'-azinobis(3-ethylenbenzothiazoline- 6-sulfonic acid) radical cation decolorization assay. Method Enzymol 299: 379-389.
  21. Benzie IFF, Strain JJ. 1996. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem 239: 70-76. https://doi.org/10.1006/abio.1996.0292
  22. Chang JH. 2006. Antioxidant activities and antiproliferative effects of various natural herb extracts. MS Thesis. Seoul National University, Seoul, Korea. p 17.
  23. Bang CS. 2007. Antioxidant and antiproliferative activities of the ethanol extracts from leafy vegetables. MS Thesis. Chungbuk National University, Chungbuk, Korea. p 24.
  24. Lee SH, Hwang IG, Lee YR, Joung EM, Jeong HS, Lee HB. 2009. Physicochemical characteristics and antioxidant activity of heated radish (Raphanus sativus L.) extracts. J Korean Soc Food Sci Nutr 38: 490-495. https://doi.org/10.3746/jkfn.2009.38.4.490
  25. Yang J, Meyers JK, Heide JV, Liu RH. 2004. Varietal differences in phenolic content and antioxidant and antiproliferative activities of onions. J Agric Food Chem 52: 6787-6793. https://doi.org/10.1021/jf0307144
  26. Bae JS, Kim TH. 2011. Pancreatic lipase inhibitory and antioxidant activities of Zingiber officinale extracts. Korean J Food Preserv 18: 390-396. https://doi.org/10.11002/kjfp.2011.18.3.390
  27. Lee JH. 1993. Studies on the content of phenolic substances on plant foods and their physiological effects in vitro. PhD Dissertation. Ewha Womans University, Seoul, Korea. p 51-53.
  28. Kang DY, Shin MO, Shon JH, Bae SJ. 2009. The antioxidative and antimicrobial effects of Celastrus orbiculatus. J Life Sci 19: 52-57. https://doi.org/10.5352/JLS.2009.19.1.052
  29. Lim SJ. 2002. Screening for antioxidant activity and antimutagenic effect of 15 vegetables. MS Thesis. Hannam University, Daejeon, Korea. p 48.
  30. Kang YH, Park YK, Lee GD. 1996. The nitrite scavenging and electron donating ability of phenolic compounds. Korean J Food Sci Technol 33: 626-632.
  31. Meller NJ, Rice-Evans C, Davies MJ, Gopinathan V, Milner A. 1993. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin Sci 84: 407-412. https://doi.org/10.1042/cs0840407
  32. Moon GS, Ryu BM, Lee MJ. 2003. Components and antioxidant activities of Buchu (Chinese chives) harvested at different times. Korean J Food Sci Technol 35: 493-498.
  33. Sachez-Gonazaez I, Jimenez-Escrig A, Saura-Calixto F. 2005. In vitro antioxidant activity of coffees brewed using different procedures (Italian, espresso and filter). Food Chem 90: 133-139. https://doi.org/10.1016/j.foodchem.2004.03.037
  34. Ahn MS, Kim HJ, Seo MS. 2005. The antioxidant and antimicrobial activities of the three species of leeks (Allium tuberosub R.) ethanol extracts. Korean J Food Culture 20: 186-193.
  35. Kim SJ, Park KH. 1995. Antimicrobial activities of the extracts of vegetable kimchi stuff. Korean J Food Sci Technol 27: 216-220.
  36. Lee EH, Jang KI, Bae IY, Lee H. 2011. Antibacterial effects of leek and garlic juice and powder in a mixed strains system Korean. J Food Sci Technol 43: 518-523.
  37. Hong JH, Lee MH, Kang MC, Hur SH. 2000. Separation and identification of antimicrobial compounds from Korean leek (Allium tuberosum). J Fd Hyg Safety 15: 235-240.
  38. Son JY. 2010. Antioxidant and antimicrobial activities of methanol extracts from spices. J Korean Soc Food Sci Nutr 39: 648-654. https://doi.org/10.3746/jkfn.2010.39.5.648
  39. Sohn HY, Kum EJ, Ryu HY, Jeon SJ, Kim NS, Son KH. 2006. Antifungal activity of fistulosides, steroidal saponins from Allium fistulosum L. J Life Sci 16: 310-314. https://doi.org/10.5352/JLS.2006.16.2.310
  40. Jung MS, Lee GS, Chae HJ. 2004. In vitro biological activity assay of ethanol extract of radish. J Korean Soc Appl Biol Chem 47: 67-71.
  41. Park KS, Kyung KH. 1992. Growth stimulation of lactic acid bacteria by a radish component. Korean J Food Sci Technol 24: 528-534.

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