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Antiproliferative effect of Schisandrae Fructus extract on PC-3 human prostate cancer cells

오미자(五味子) 추출물의 인간 전립선암 세포주 PC-3에 대한 성장 억제 효과

  • Moon, Jung-Min (School of Korean Medicine, Pusan National University) ;
  • Seok, Ga-Hyeong (School of Korean Medicine, Pusan National University) ;
  • Cho, Su-In (School of Korean Medicine, Pusan National University)
  • 문정민 (부산대학교 한의학전문대학원) ;
  • 석가형 (부산대학교 한의학전문대학원) ;
  • 조수인 (부산대학교 한의학전문대학원)
  • Received : 2012.06.08
  • Accepted : 2012.06.26
  • Published : 2012.07.30

Abstract

Objectives : Schisandrae Fructus (SF) has traditionally been used to balance level of body fluid and to strengthen kidney function. It has been reported that the SF extract has antioxidant, hepatoprotective, neuroprotective and anticancer effects. This study investigated an antiproliferative effect of SF extract on PC-3 human prostate cancer cells and analyzed active ingredients of SF extract qualitatively and quantitatively. Methods : We examined the antiproliferative effect of SF extract with MTT assay, DAPI staining and annexin-V/7-AAD double staining. The active ingredients of SF extract were identified by using HPTLC and HPLC/DAD system. Results : SF-chloroform fraction inhibited growth of PC-3 cells and changed the morphology of nucleus in a dose dependent manner. A dose-dependent apoptotic cell death was also measured by flow cytometry analysis. It was analyzed that SF-chloroform fraction contained more schizandrin than other fractions by using HPTLC and HPLC/DAD system. Conclusions : These results suggest that SF extract and schizandrin may be a potential chemotherapeutic agent for the control of PC-3 human prostate cancer cells.

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