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DOI QR Code

An Experimental Model for Induction of Lung Cancer in Rats by Chlamydia Pneumoniae

  • Chu, De-Jie (Department of Pulmonary Medicine, Jinshan Hospital) ;
  • Guo, Shui-Gen (Department of Pulmonary Medicine, Jinshan Hospital) ;
  • Pan, Chun-Feng (Department of Pulmonary Medicine, Jinshan Hospital) ;
  • Wang, Jing (Department of Pulmonary Medicine, Jinshan Hospital) ;
  • Du, Yong (Department of Pulmonary Medicine, Jinshan Hospital) ;
  • Lu, Xu-Feng (Department of Pulmonary Medicine, Jinshan Hospital) ;
  • Yu, Zhu-Yuan (Zhongshan Hospital, Fudan University)
  • Published : 2012.06.30

Abstract

Objective: To assess induction effects of Chlamydia pneumoniae (Cpn) on lung cancer in rats. Methods: A lung cancer animal model was developed through repeated intratracheal injection of Cpn (TW-183) into the lungs of rats, with or without exposure to benzo(a)pyrene (Bp). Cpn antibodies (Cpn-IgA, -IgG, and -IgM) in serum were measured by microimmunofluorescence. Cpn-DNA or Cpn-Ag of rat lung cancer was detected through polymerase chain reaction or enzyme-linked immunosorbent assay. Results: The prevalence of Cpn infection was 72.9% (35/48) in the Cpn group and 76.7% (33/43) in the Cpn plus benzo(a)pyrene (Bp) group, with incidences of lung carcinomas in the two groups of 14.6% (7/48) and 44.2% (19/43), respectively (P-values 0.001 and <0.000 compared with normal controls). Conclusions: A rat model of lung carcinoma induced by Cpn infection was successfully established in the laboratory for future studies on the treatment, prevention, and mechanisms of the disease.

Keywords

Chlamydia pneumonia;benzo (a) pyrene;lung cancer;animal model;rat

Acknowledgement

Supported by : Shanghai Municipal Science and Technology Commission

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