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New Model of In-situ Xenograft Lymphangiogenesis by a Human Colonic Adenocarcinoma Cell Line in Nude Mice

  • Sun, Jian-Jun (Department of Surgery, Tongji Hospital, Tongji University School of Medicine) ;
  • Jing, Wei (Department of Surgery, Tongji Hospital, Tongji University School of Medicine) ;
  • Ni, Yan-Yan (Department of Surgery, Tongji Hospital, Tongji University School of Medicine) ;
  • Yuan, Xiao-Jian (Department of Surgery, Tongji Hospital, Tongji University School of Medicine) ;
  • Zhou, Hai-Hua (Department of Surgery, Tongji Hospital, Tongji University School of Medicine) ;
  • Fan, Yue-Zu (Department of Surgery, Tongji Hospital, Tongji University School of Medicine)
  • Published : 2012.06.30

Abstract

Objective: To explore a new model of in-situ xenograft lymphangiogenesis of human colonic adenocarcinomas in nude mice. Method: On the basis of establishing subcutaneous xenograft lymphangiogenesis model of human colonic adenocarcinoms, in-situ xenografts were established through the in situ growth of the HT-29 human colonic adenocarcinoma cell line in nude mice. The numbers of lymphangiogenic microvessels, the expression of lymphatic endothelial cell markers lymphatic vessel endothelial hyaloronic acid receptor-1 (LYVE-1), D2-40 and the lymphatic endothelial growth factors vascular endothelial growth factor-C (VEGF-C), -D (VEGF-D) and receptor-3 (VEGFR-3) were compared by immunohistochemical staining, Western bolt and quantitative RT-PCR in xenograft in-situ models. Results: Some microlymphatics with thin walls, large and irregular or collapsed cavities and increased LMVD, with strong positive of LYVE-1, D2-40 in immunohistochemistry, were observed, identical with the morphological characteristics of lymphatic vessels and capillaries. Expression of LYVE-1 and D2-40 proteins and mRNAs were significantly higher in xenograpfts in-situ than in the negative control group(both P<0.01). Moreover, the expression of VEGF-C, VEGF-D and VEGFR-3 proteins and mRNAs were significantly higher in xenografts in-situ (both P<0.01), in conformity with the signal regulation of the VEGF-C,-D/VEGFR-3 axis of tumor lymphangiogenesis. Conclusions: In-situ xenografts of a human colonic adenocarcinoma cell line demonstrate tumor lymphangiogenesis. This novel in-situ animal model should be useful for further studying mechanisms of lymph node metastasis, drug intervention and anti-metastasis therapy in colorectal cancer.

Keywords

Colonic neoplasm;models;animal;lymphangiogenesis

Acknowledgement

Supported by : National Natural Science

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