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Biological Response Modifiers Influence Structure Function Relationship of Hematopoietic Stem and Stromal Cells in a Mouse Model of Leukemia

  • Basu, Kaustuv (Department of Anatomy and Cell Biology, McGill University) ;
  • Mukherjee, Joydeep (Brain Tumor Research Centre, Department of Neurosurgery, Helen Diller Family Comprehensive Cancer Centre, UCSF) ;
  • Law, Sujata (Department of Biochemistry & Medical Biotechnology, Stem Cell Research & Application Unit, Calcutta School of Tropical Medicine) ;
  • Chaudhuri, Samaresh (Department of Biochemistry & Medical Biotechnology, Stem Cell Research & Application Unit, Calcutta School of Tropical Medicine)
  • Published : 2012.06.30

Abstract

Biological response modifiers (BRMs) can alter interactions between the immune system and cancer cells to boost, direct, or restore the body's ability to fight disease. Mice with ethylnitrosourea- (ENU) induced leukemia were here used to monitor the therapeutic efficacy of lipopolysaccaride (LPS), Bacillus Calmette Guerin (BCG) and sheep erythrocytes (SRBC). Flow cytometry based CD34+ positivity analysis, clonogenicity, proliferation and ultrastructure studies using scanning electron microscopy (SEM) of stem cells in ENU induced animals with and without BRMs treatment were performed. BRMs improved the stem-stromal relationship structurally and functionally and might have potential for use as an adjunct in human stem cell therapy.

Keywords

Biological response modifiers;ENU-induced leukemia;LPS;BCG;SRBC

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