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Study on Antioxidant Effects of Fractional Extracts from Ligularia stenocephala Leaves

곤달비(Ligularia stenocephala) 잎 분획물의 항산화활성 평가

  • Kim, Kyoung-Hee (Dept. Of Food and Nutrition, Chungnam National University) ;
  • Kim, Na-Young (Dept. of Food and Nutrition, Jungbu University) ;
  • Kim, Sung-Hwan (Dept. of Food and Nutrition, Jungbu University) ;
  • Han, In-Ae (Dept. Of Food and Nutrition, Chungnam National University) ;
  • Yook, Hong-Sun (Dept. Of Food and Nutrition, Chungnam National University)
  • 김경희 (충남대학교 식품영양학과) ;
  • 김나영 (중부대학교 식품영양학과) ;
  • 김성환 (중부대학교 식품영양학과) ;
  • 한인애 (충남대학교 식품영양학과) ;
  • 육홍선 (충남대학교 식품영양학과)
  • Received : 2012.05.08
  • Accepted : 2012.06.18
  • Published : 2012.09.30

Abstract

Ligularia stenocephala leaves were subjected to extraction by using several solvents with different polarities, which were then investigated for their antioxidant and antimicrobial activities. Extraction yield of ethanol extract of Ligularia stenocephala was 21.36%. The water fraction showed the highest extraction yield of 60.35%, followed by the n-butanol, n-hexane, and ethyl acetate fractions. Total polyphenolic content was the highest (254.00 mg/g GAE) in the ethyl acetate fraction of Ligularia stenocephala extract. The ethyl acetate fraction had $IC_{50}$ values of 0.28 mg/mL for DPPH radical scavenging, and 96.$67{\pm}0.09%$ for ABTS radical scavenging activity. Further, FRAP value was significantly higher in the ethyl acetate fraction. The ethyl acetate fraction showed antimicrobial activities against B. cereus, and the diameter of the zone of inhibition was 10.2 mm at 5 mg/disc. These results suggest that the ethyl acetate fraction of Ligularia stenocephala possesses the antimicrobial activities against B. cereus, and its high antioxidant activity could be applicable to food additives and as a natural cosmetic ingredient.

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