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Eastern Staining: A Simple Recombinant Protein Detection Technology Using a Small Peptide Tag and Its Counter Partner Which is a Fluorescent Compound

  • Lee, Jae-Jung (Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research) ;
  • Kim, Jun-Young (Department of Chemistry, MedChem Program of Life Sciences Institute, National University of Singapore) ;
  • Zhai, Duanting (Department of Chemistry, MedChem Program of Life Sciences Institute, National University of Singapore) ;
  • Yun, Seong-Wook (Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research) ;
  • Chang, Young-Tae (Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research)
  • Received : 2012.06.01
  • Accepted : 2012.06.15
  • Published : 2012.06.30

Abstract

Small peptide tags such as c-myc, HA, or FLAG tag have facilitated efficient Western-blotting of proteins of interest especially when specific antibodies for the proteins are not available. However, the conventional Western-blotting requires the multi-steps process taking at least several hours up to two days. With examples of various applications, here we show a convenient and time-saving method for protein detection which employs a fluorescent chemical BDED and its binding peptide RC-tag. And we propose "Estern staining", as a standard term for protein detection method using fluorescent chemicals and their binding small peptide tags. Eastern staining may substitutes for the time-consuming "immuno-staining" in many versatile applications.

Acknowledgement

Supported by : A*STAR Biomedical Research Council

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