DOI QR코드

DOI QR Code

Effects of Replacement of Fish Meal by Soy Protein Isolate on the Growth, Digestive Enzyme Activity and Serum Biochemical Parameters for Juvenile Amur Sturgeon (Acipenser schrenckii)

  • Xu, Q.Y. (Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences) ;
  • Wang, C.A. (Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences) ;
  • Zhao, Z.G. (Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences) ;
  • Luo, L. (Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences)
  • Received : 2012.03.27
  • Accepted : 2012.06.13
  • Published : 2012.11.01

Abstract

An 8-wk experiment was conducted to evaluate the effect of replacing fish meal (FM) with soy protein isolate (SPI) on the growth, digestive enzyme activity and serum biochemical parameters of juvenile Amur sturgeon (Acipenser schrenckii). SPI was used to replace 0, 25, 50, 62.5, 75, 87.5, 100% of dietary FM and 100% replacement supplemented crystalline amino acid. Healthy sturgeon with an average initial weight of $26.38{\pm}0.24$ g were randomly assigned to 24 aquaria (8 treatments with triplicates each) at an initial stocking density of 11 fish per aquarium and cultured for 8 wks. The results showed that 75.00% or more substitution resulted in a poor weight gain rate, feed conversion ratio and survival rate compared to that of fish fed the control diet (p<0.05), whereas no significant differences were observed between diets of 25.00% to 62.50% substitution. Protease, lipase and amylase activity in foregut, mid-gut and hindgut were significantly (p<0.05) decreased by diets where SPI replacement levels were 62.50% or more. Levels of serum total protein (TP) and globulin decreased significantly from 21.03, 10.34 to 14.05, 5.63 g/L with the increasing dietary SPI (p<0.05), but alkaline phosphatase activity significantly increased (p<0.05). In addition, supplemental crystalline amino acid in the FM absence diet did not improve growth performance, intestine digestive enzyme activities and serum biochemical parameters. In conclusion, the results from this study showed adverse effects of inclusion of SPI in diets on growth performance, feed utilization and serum biochemical parameters in juvenile Amur sturgeon. Based on WGR and replacement ratio presented in this report, a 57.64% replacement level was recommended.

Keywords

Soy Protein Isolate;Acipenser schrenckii;Growth;Digestive Enzyme Activity;Serum Biochemical Parameters

References

  1. Alexandar, J. B. and G. A. Ingram. 1992. Noncellular non-specific defense mechanisms of fish. Annu. Rev. Fish Dis. 2:249-279. https://doi.org/10.1016/0959-8030(92)90066-7
  2. Anver, C. E. 2004. Blood chemistry (electrolytes, lipoprotein and enzymes) values of black scorpion fish (Scorpaena porcus, 1758) in the Dardanelles. Turkey. J. Biol. Sci. 4:716-719. https://doi.org/10.3923/jbs.2004.716.719
  3. Ao, X., H. J. Kim, Q. Meng, W. L. Yan, J. H. Cho and I. H. Kim. 2010. Effects of diet complexity and fermented soy protein on growth performance and apparent ileal amino acid digestibility in weanling pig. Asian-Aust. J. Anim. Sci. 23:1496-1502. https://doi.org/10.5713/ajas.2010.10109
  4. Asadi, F., M. Masoudifard, A. Vajhi, K. Lee, M. Pourkabir and P. Khazraeinia. 2006. Serum biochemical parameters of Acipenser persicus. Fish Physiol. Biochem. 32:43-47. https://doi.org/10.1007/s10695-005-5738-0
  5. Bai, S. C. and D. M. Gatlin. 1994. Effects of L-lysine supplementation of diets with different protein levels and sources on channel catfish, Ictalurus puncrarus (Rafinesque). Aquac. Res. 25:465-474. https://doi.org/10.1111/j.1365-2109.1994.tb00711.x
  6. Burtis, C. A. and E. R. Ashwood. 1996. Tietz Fundamentals of Clinical Chemistry. W.B. Saunders Company, Philadelphia.
  7. Carter, C. G. and R. C. Hauler. 2000. Fish meal replacement by plant meals in extruded feeds for Atlantic salmon, Salmo salar L. Aquaculture. 185:299-311. https://doi.org/10.1016/S0044-8486(99)00353-1
  8. Cowey, C. B. and M. J. Walton. 1988. Studies on the uptake of ($^{14}C$) amino acids derived from both dietary ($^{14}C$) protein and dietary ($^{14}C$) amino acids by rainbow trout, Salmo gairdneri Richardson. J. Fish Biol. 33:293-305. https://doi.org/10.1111/j.1095-8649.1988.tb05472.x
  9. Dabrowski, K. and H. Guderley. 2002. Intermediary metabolism. In: Fish Nutrition (Ed. J. E. Halver and R. W. Hardy). Academic Press, San Diego, CA, USA, pp. 310-367.
  10. Decie, S. IV. and S. M. Lewis. 1991. Practical haematology, (VII edn). Churchill, Livingston.
  11. El-Saidy, D. M. S. and M. M. Gaber. 1997. Total replacement of fish meal by soybean meal, with various percentages of supplemental L-methionine, in diets for Nile tilapia (oreochromisniloticus, L.). Annals of Agriculture Science of Moshtohor 35:1 223-1 238.
  12. El-Saidy, D. M. S. and M. M. Gaber. 2002. Complete replacement of fishmeal by soybean with the dietary L-lysine supplementation in Nile tilapia fingerlings. J. World Aquac. Soc. 33:297-306. https://doi.org/10.1111/j.1749-7345.2002.tb00506.x
  13. Floreto, E. A. T., R. C. Bayer and P. B. Brown. 2000. The effects of soybean-based diets, with and without amino acid supplementation, on growth and biochemical composition of juvenile American Lobster, Homarus americanus. Aquaculture 189:211-235. https://doi.org/10.1016/S0044-8486(00)00363-X
  14. Gjellesvik, D. R., D. Lombardo and B. T. Walther. 1992. Pancreatic bile salt dependent lipase from cod (Gadus morhua): purification and properties. Biochim. Biophys. Acta. 1124:123-134. https://doi.org/10.1016/0005-2760(92)90088-D
  15. Goss, G. G. and C. M. Wood. 1988. The effects of acid and acid/aluminium exposure on circulating plasma cortisol levels and other blood parameters in the rainbow trout, Salmo gairdneri. J. Fish Biol. 32:63-76. https://doi.org/10.1111/j.1095-8649.1988.tb05335.x
  16. Haard, N. F., L. E. Dimes, R. E. Arndt and F. M. Dong. 1996. Estimation of protein digestibility. IV. Digestive proteinases from the pyloric caeca of coho salmon (Oncorhynchus kisutch) fed diets containing soybean meal. Comp. Biochem. Physiol. B 115:533-540. https://doi.org/10.1016/S0305-0491(96)00189-7
  17. Kaushik, S. J. 1990. Use of alternative protein sources for the intensive rearing of carnivorous fish. In: Mediterranean Aquaculture (Ed. R. Flos, L. Tort and P. Torres), pp. 125-138. Ellis Horwood.
  18. Krogdahl, A. and H. Holm. 1981. Soybean protease inhibitors and human proteolytic enzymes: selective inactivation of inhibitors by treatments with human gastric juice. J. Nutr. 111:2045-2051.
  19. Krogdahl, A. and H. Holm. 1981. Soybean protease inhibitors and human proteolytic enzymes: selective inactivation of inhibitors by treatments with human gastric juice. J. Nutr. 111:2045-2051.
  20. Liu, Y. M., J. Z. Zhu, H. Y. Wu and D. Z. Shi. 1991. Studies on digestive enzymes and amino acid of larval and post larval stages of prawn Penaeus chinensis. Oceanol. Lminol. Sin. 22:571-575.
  21. Mambrini, M., A. J. Roem, J. P. Cravedi, J. P. Lalles and S. J. Kaushik. 1999. Effect of replacing fish meal with soy protein concentrate and of DL-methionine supplementation in high-energy, extruded diets on the growth and nutrient utilization of rainbow trout, Oncorhynchus mykiss. J. Anim. Sci. 77:2990-2999.
  22. Min, B. J., J. H. Cho, Y. J. Chen, H. J. Kim, Y. S. Yoo, C. Y. Lee, B. C. Park, J. H. Lee and I. H. Kim. 2009. Effects of fermented soy protein on growth performance and blood protein contents in nursery pigs. Asian-Aust. J. Anim. Sci. 22:1038-1042. https://doi.org/10.5713/ajas.2009.80240
  23. Morales, A. E., G. Cardenete, M. de la Higuera and A. Sanz. 1994. Effects of dietary protein source on growth, feed conversion and energy utilisation in rainbow trout, Oncorhynchus mykiss. Aquaculture 124:117-126. https://doi.org/10.1016/0044-8486(94)90367-0
  24. Naylor, R. L., R. J. Goldberg, J. H. Primavera, N. Kautsky, M. C. M. Beveredge, J. Clay, C. Folke, J. Lubchenco, H. Mooney and M. Troell. 2000. Effects of aquaculture on world fish supplies. Nature 405:1017-1 024. https://doi.org/10.1038/35016500
  25. Neff, J. M. 1985. Use of biochemical measurement to detect pollutant-mediated damage to fish. ASTM Spec Tech Pub. 1 854:155-183.
  26. Olli, J., K. Hjelmeland and A. Krogdahl. 1994. Soybean trypsin inhibitor in diets for Atlantic salmon (Salmo salar, L): Effects on nutrient digestibilities and in pyloric caeca homogenate and intestinal content. Comp. Biochem. Physiol. A 109:923-928. https://doi.org/10.1016/0300-9629(94)90240-2
  27. Rehulka, J. 2000. Influence of astaxanthin on growth rate, condition, and some blood indices of rainbow trout, Oncorhynchus mykiss. Aquaculture 190:27-47. https://doi.org/10.1016/S0044-8486(00)00383-5
  28. Shiau, S. Y., J. I. Chuang and C. L. Sun. 1987. Inclusion of soybean meal in tilapia, 0. niloricus X 0. aureus diets at two protein levels. Aquaculture 65:251-261. https://doi.org/10.1016/0044-8486(87)90238-9
  29. Ueberschaer, B. and C. Clemmesen. 1992. A comparison of the nutritional condition of herring larvae as determined by two biochemical methods - tryptic enzyme activity and RNA/DNA ratio measurements. ICES J. Mar. Sci. 49:245-249. https://doi.org/10.1093/icesjms/49.2.245
  30. Ueberschaer, B., B. H. Pedersen and K. Hjelmeland. 1992. Quantification of trypsin with radioimmunoassay in herring larvae (Clupea harengus) compared with a highly sensitive fluorescent technique to determine tryptic enzyme activity. Mar. Biol. 113:469-473. https://doi.org/10.1007/BF00349173
  31. Vajcova, V., S. Navrati and M. Palıkova. 1998. The effect of intraperitoneally applied puremicrocystin-LRon haematological, biochemical and morphological indices of silver carp (Hypophthalmichthys molitrix Val.). Acta Vet. Brno. 67:281-287. https://doi.org/10.2754/avb199867040281
  32. Viola, S. and E. Lahav. 1991. The protein sparing effect of synthetic lysine in practical carp feeds. Fish nutrition in practice. IV International Symposium on Fish Nutrition and Feeding, Bianitz, France. Abstract.
  33. Wagner, T. and J. L. Congleton. 2004. Blood chemistry correlates of nutritional condition, tissue damage, and stress in migrating Juvenile Chinook salmon (Onchorhynchus tshawytscha). Can. J. Fish. Aquat. Sci. 61:1066-1074. https://doi.org/10.1139/f04-050
  34. Whitaker, J. R. 1994. Principles of enzymology for the food sciences. 2nd ed. Marcel Dekker, New York, USA.
  35. Xu, B. H., Y. B. Wang, J. R. Li and Q. Lin. 2009. Effect of prebiotic xylooligosaccharides on growth performances and digestive enzyme activities of allogynogenetic crucian carp (Carassius auratus gibelio). Fish Physiol. Biochem. 35:351-357. https://doi.org/10.1007/s10695-008-9248-8
  36. Zhang, J. X., X. Q. Zhou, X. Q. Ni and Y. Liu. 2008. Effect of soybean protein isolate on growth performance and intestine of Cyprinus carpio var. jian juveniles. J. Fish. China. 32:84-90.
  37. Zhuang, P., B. Kynard, L. Zhang, T. Zhang, Z. Zhang and D. Li. 2002. Overview of biology and aquaculture of Amur sturgeon (Acipenser schrenckii) in China. J. Appl. Ichthyol. 18:659-664. https://doi.org/10.1046/j.1439-0426.2002.00365.x
  38. Zeitoun, H. I., D. E. Ullrey and W. T. Magee. 1976. Quantifying nutrient requirements of fish. J. Fish. Res. Board Can. 33:167-172. https://doi.org/10.1139/f76-019
  39. Zou, Y. R., Q. H. Ai, K. S. Mai, W. B. Zhang, Y. J. Zhang and W. Xu. 2012. Effects of brown fish meal replacement with fermented soybean meal on growth performance, feed efficiency and enzyme activities of Chinese soft-shelled turtle, Pelodiscus sinensis. J. Ocean Univ. China. 11:227-235 (DOI 10.1007/s11802-012-1896-8). https://doi.org/10.1007/s11802-012-1896-8

Cited by

  1. Effects of fish meal replacement by soybean peptide on growth performance, digestive enzyme activities, and immune responses of yellow catfish Pelteobagrus fulvidraco vol.82, pp.4, 2016, https://doi.org/10.1007/s12562-016-0996-6
  2. pp.13535773, 2017, https://doi.org/10.1111/anu.12645
  3. pp.13535773, 2018, https://doi.org/10.1111/anu.12839
  4. L pp.1355557X, 2018, https://doi.org/10.1111/are.13847
  5. Effects of dietary myo-inositol on growth, chemical composition and plasma chemistry of Amur sturgeon Acipenser schrenckii pp.1573-143X, 2018, https://doi.org/10.1007/s10499-018-0299-2
  6. Growth Performance, Digestive Enzymes, and TOR Signaling Pathway of Litopenaeus vannamei Are Not Significantly Affected by Dietary Protein Hydrolysates in Practical Conditions vol.9, pp.1664-042X, 2018, https://doi.org/10.3389/fphys.2018.00998
  7. ♂ vol.24, pp.4, 2018, https://doi.org/10.1111/anu.12674
  8. Changes in serum biochemical parameters and digestive enzyme activity of juvenile sobaity sea bream (Sparidentex hasta) in response to partial replacement of dietary fish meal with poultry by-product meal pp.1573-5168, 2019, https://doi.org/10.1007/s10695-019-00619-4