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Antioxidative and Physiological Activities of Fractions from Pleurospermum kamtschaticumin Extracts

누룩치(Pleurospermum kamtschaticumin) 추출물의 항산화 효과 및 생리활성

  • Kim, Mi-Seon (Dept. of Food and Nutrition, Chungnam National University) ;
  • Kim, Kyoung-Hee (Dept. of Food and Nutrition, Chungnam National University) ;
  • Yook, Hong-Sun (Dept. of Food and Nutrition, Chungnam National University)
  • 김미선 (충남대학교 식품영양학과) ;
  • 김경희 (충남대학교 식품영양학과) ;
  • 육홍선 (충남대학교 식품영양학과)
  • Received : 2012.06.28
  • Accepted : 2012.07.19
  • Published : 2012.10.31

Abstract

This study investigated the antioxidant activities, whitening effects, and antimicrobial activities of fractions from Pleurospermum kamtschaticumin extract. Total polyphenolic contents of fractions from Pleurospermum kamtschaticumin extract were 116.44~382.73 mg/g GAE (gallic acid equivalent), with the highest value in the ethyl acetate fraction. Fractions of Pleurospermum kamtschaticumin showed the highest DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity ($IC_{50}$=0.04 mg/mL) and ABTS radical scavenging activity (98.86% at 0.5 mg/mL), which was similar to ascorbic acid. Further, reducing power and FRAP activity were significantly high in the ethyl acetate and n-butanol fractions. The ethyl acetate and n-butanol fractions showed significantly high SOD-like activities (1 mg/mL, 86.93%, and 78.23%, respectively) compared to other fractions. Tyrosinase inhibition activities of the n-butanol fraction and 80% ethanol extract were 43.52% and 41.64%, respectively. Antimicrobial activity against Escherichia coli was only observed in the chloroform fraction (16.7 mm in 0.5 mg/disc). These results suggest that fractions from Pleurospermum kamtschaticumin extract show comparatively high antioxidant activity and thus could be used as a food additive or cosmeceutical ingredient.

References

  1. Ames BN. 1989. Endogenous oxidative DNA damage, aging, and cancer. Free Radical Res Commun 7: 121-128. https://doi.org/10.3109/10715768909087933
  2. Cha BC, Lee HW, Choi MY. 1998. Antioxidative and antimicrobial effects of nut species. Kor J Pharmacogn 29: 28-34.
  3. Chang SS, Ostric-Matijasevic B, Hsieh OAL, Huang CL. 1977. Natural antioxidants from rosemary and sage. J Food Sci 42: 1102-1106. https://doi.org/10.1111/j.1365-2621.1977.tb12676.x
  4. Jeong SJ, Lee JH, Song NH, Seong SN, Lee SE, Baeg NI. 2004. Natural products organic chemistry; screening for antioxidant activity of plant medicinal extracts. J Korean Soc Appl Biol Chem 47: 135-140.
  5. Kim EJ, Ahn MS. 1993. Antioxidative effect of ginger extracts. Korean J Soc Food Sci 9: 37-42.
  6. Rural Development Administration. 1999. Wild vegetables cultivation (Standard Farming Handbook-60). Suwon, Korea. p 110-111.
  7. Lim SJ, Park NJ. 1994. A study on the development of new recipes of 5-Korean wild vegetables. Korean J Soc Food Sci 10: 412-419.
  8. Heo SI, Wang MH. 2008. Antioxidant activity and cytotoxicity effect of extracts from Taraxacum mongolicum H. Kor J Pharmacogn 39: 255-259.
  9. Hendrich S, Lee KW, Xu X, Wang HJ, Murphy PA. 1994. Defining food components as new nutritions. J Nutr 124: 1789S-1792S.
  10. Ueda S, Kuwabara Y, Hirai N, Sasaki H, Sugahara T. 1991. Antimutagenic capacities of different kinds of vegetables and mushrooms. Nihon Shokubin Kogyoo Cakkaishi 38: 507-514.
  11. Hwang BH, Zhao JL, Choi KP, Jung SW, Kim EJ, Ham SS. 1996. The antimutagenic and anticancer effect of Taxus cuspidate extracts. J Korean Soc Food Nutr 25: 1062-1068.
  12. Jhee OH, Yang CB. 1996. Antioxidative activity of extract from Bangah herb. Korean J Food Sci Technol 28: 1157- 1163.
  13. Lee BW, Shin DH. 1991. Screening of natural antimicrobial plant extract on food spoilage microorganisms. Korean J Food Sci Technol 23: 200-204.
  14. Ham SS, Lee SY, Oh DW, Jung SW, Kim SH, Chung CK, Kang IJ. 1998. Antimutagenic and antigenotoxic effects of Ligularia fischeri extracts. J Korean Soc Food Sci Nutr 27: 745-750.
  15. Kim HK, Kim YE, Do JR, Lee YC, Lee BY. 1995. Antioxidative activity and physiological activity of some Korean medicinal plants. Korean J Food Sci Technol 27: 80-85.
  16. Shin DH, Kim MS, Han JS. 1997 Antimicrobial effect fethanol extracts from some medicinal herbs and their fractionates against food-born bacteria. Korean J Food Sci Technol 29: 808-816.
  17. Chung MS, Lee MS. 1998. Analysis of volatile flavor component of Pleurospermum kamtschaticum. Korean J Soc Food Sci 14: 541-546.
  18. Lim JH, Hong CK, Hong DG, Bang SB, Yoo KO. 1996. Studies on the cultivation of Pleurospermum kamtschaticum Hoff. as a special vegetable indigenous to high land area. RDA J Agri Sci 38: 31-41.
  19. Folin O, Denis W. 1912. On phospotungastic-phosphomolybdic compounds as color reagents. J Biol Chem 12: 239-243.
  20. Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200. https://doi.org/10.1038/1811199a0
  21. Pellegrin N, Roberta R, Min Y, Catherine RE. 1998. Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2'-azinobis(3-ethylenbenzothiazoline- 6-sulfonic acid) radical cation decolorization assay. Method Enzymol 299: 379-389.
  22. Benzie IFF, Strain JJ. 1996. The ferric reducing ability of plasma (FRAP) as a measure of "Antioxidant power": the FRAP assay. Anal Biochem 230: 70-76.
  23. Oyaizu M. 1986. Studies on product of browning reaction: antioxidative activities of browning reaction prepared from glucoseamine. Jap J Nutr 44: 307-315. https://doi.org/10.5264/eiyogakuzashi.44.307
  24. Marklund S, Marklund G. 1974. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47: 469-474. https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  25. Yagi A, Kanbara T, Morinobu N. 1986. The effect of tyrosinase inhibition for aloe. Plant Medica 52: 517-519.
  26. Choi YM, Kim MH, Shin JJ, Park JM, Lee JS. 2003. The antioxidant activities of the some commercial teas. J Korean Soc Food Sci Nutr 32: 723-727. https://doi.org/10.3746/jkfn.2003.32.5.723
  27. Miliauskas G, Venskutonis PR, van Beek TA. 2004. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem 85: 231-237. https://doi.org/10.1016/j.foodchem.2003.05.007
  28. Lee SO, Lee HJ, Yu MH, Im HG, Lee IS. 2005. Total polyphenol contents and antioxidant activities of methanol extracts from vegetables produced in Ullung island. Korean J Food Sci Technol 37: 233-240.
  29. Jang MJ, Woo MH, Kim YH, Jun DY, Rhee SJ. 2005. Effects of antioxidative, DPPH radical scavenging activity and antithrombogenic by the extract of sancho (Zanthoxylum schinifolium). Korean J Nutr 38: 386-394.
  30. Kim MJ, Kim JS, Cho MA, Kang WH, Jeong DM, Ham SS. 2002. Biological activity of Ixeris dentata Nakai juice extracts. J Korean Soc Food Sci Nutr 31: 924-930. https://doi.org/10.3746/jkfn.2002.31.5.924
  31. Kang MC, Lee JY, Lee JA, Han JH, Kim BS, Kim GO. 2008. Antioxidant effects and melanin inhibitory effect of natural Pimpinella komarovii extracts in Jeju island. Korean J Biotechnol Bioeng 23: 77-82.
  32. Seo YH, Kim IJ, Yie AS, Min HK. 1999. Electron donating ability and contents of phenolic compounds, tocopherols and carotenoids in waxy corn (Zea mays L.). Korean J Food Sci Technol 31: 581-585.
  33. Choi SS, Yim DS, Lee SY. 2009. Radical scavenging activities and protective effects against oxidative damage to DNA of extracts from medicinal plants with known osteoprotective effects. Kor J Pharmacogn 40: 143-149.
  34. Meir S, Kanner J, Akiri B, Philosoph-Hadas S. 1995. Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves. J Agric Food Chem 43: 1813-1819. https://doi.org/10.1021/jf00055a012
  35. Schlesier K, Harwat M, Bohm V, Bitsch R. 2002. Assessment of antioxidant activity by using different in vitro methods. Free Radic Res 36: 177-187. https://doi.org/10.1080/10715760290006411
  36. Diplock AT. 1997. Will the 'good fairies' please prove to us that vitamin E lessens human degenerative disease? Free Radic Res 27: 511-532.
  37. Choi JI, Kim YJ, Kim JH, Song BS, Yoon Y, Byun MW, Kwon JH, Chun SS, Lee JW. 2009. Antioxidant activities of the extract fractions from Suaeda japonica. J Korean Soc Food Sci Nutr 38: 131-135. https://doi.org/10.3746/jkfn.2009.38.2.131
  38. Moon GS, Ryu BM, Lee MJ. 2003. Components and antioxidative activities of Buchu (Chines chives) harvested at different times. Korean J Food Sci Technol 35: 493-498.
  39. Sachez-Gonzaez I, Jimenez-Escrig A, Saura-Calixto F. 2005. In vitro antioxidant activity of coffees brewed using different procedures (Italian, espresso and filter). Food Chem 90: 133-139. https://doi.org/10.1016/j.foodchem.2004.03.037
  40. Lim JD, Yu CY, Kim MJ, Yun SJ, Lee SJ, Kim NY, Chung IM. 2004. Comparison of SOD activity and phenolic compound contents in various Korean medicinal plants. Korean J Medicinal Crop Sci 12: 191-202.
  41. An BJ, Lee JT. 2002. Studies on biological activity from extract of Crataegi Fructus. Kor J Herbology 17: 29-38.
  42. Hong HD, Kand NK, Kim SS. 1998. Superoxide dismutaselike activity of apple juice mixed with some fruits and vegetables. J Korean Food Sci Technol 30: 1484-1487.
  43. Choi BW, Kee BH, Kang JH, Lee ES, Lee NH. 1998. Screening of the tyrosinase inhibitors from marine algae and medicinal plants. Kor J Pharmacogn 29: 237-242.
  44. Park YH, Chang SK. 1997. Screening of inhibitory effect of edible mushrooms on tyrosinase and isolation of active component. J Fd Hyg Safety 12: 195-199.
  45. Lee YS, Joo EY, Kim NW. 2006. Polyphenol contents and physiological activity of the Lespedeza bicolor extracts. Korean J Food Preserv 13: 616-622.
  46. Roh EJ, Kim YS, Kim BG. 2009. Effect of antioxidation and inhibition of melanogenesis form Ligularia stenocephala extract. J Korean Oil Chemists' Soc 26: 87-92.
  47. Kim KH, Chun HJ, Han YS. 1998. Screening of antimicrobial activity of the dandelion (Taraxacum platycarpum) extract. Korean J Soc Food Sci 141: 114-118.
  48. Lim HA, Yun SI. 2009. Antimicrobial activities of Capsella bursa-pastoris extracts. Korean J Food Preserv 16: 562-566.

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