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건조방법에 따른 꾸지뽕열매 추출물의 항산화활성과 Angiotensin Converting Enzyme I 저해활성

Antioxidant and Angiotensin Converting Enzyme I Inhibitory Activities of Extracts from Mulberry (Cudrania tricuspidata) Fruit subjected to Different Drying Methods

  • 윤광섭 (대구가톨릭대학교 식품가공학전공) ;
  • 김재원 (대구가톨릭대학교 식품가공학전공)
  • Youn, Kwang-Sup (Dept. of Food Science and Technology, Catholic University of Daegu) ;
  • Kim, Jae-Won (Dept. of Food Science and Technology, Catholic University of Daegu)
  • 투고 : 2012.06.20
  • 심사 : 2012.07.16
  • 발행 : 2012.10.31

초록

건조 꾸지뽕나무 열매의 기능성 소재으로서의 개발 가능성을 알아보고자 70% ethanol 추출물에 대한 생리활성을 측정하였다. 실험군은 꾸지뽕나무 열매 동결건조분말 70% ethanol 추출물(ECFD), 열풍건조분말 70% ethanol 추출물(ECHD), 적외선건조분말 70% ethanol 추출물(ECID)을 각각 동결건조하여 실험에 사용하였다. 수율은 적외선건조에서 높은 수율을 나타내었다. 색도는 $L^*$, $a^*$, $b^*$$H^{\circ}$ 값 모두 열풍건조 및 적외선건조에서 감소하였으며, 총 폴리페놀 및 플라보노이드 함량은 적외선건조에서 가장 높은 함량을 나타내었다. 전자공여능은 적외선건조에서 높은 활성을 나타내었으며, 환원력 및 ABTS 라디칼 소거활성에서도 유사한 경향을 나타내었다. 아질산염 소거능은 적외선건조, 열풍건조, 동결건조 순으로 높은 활성을 나타내었다. SOD 유사활성은 동결건조에서는 611.97 mUnit, 열풍건조에서는 723.02 mUnit, 적외선건조에서는 891.93 mUnit로 열풍건조 및 적외선건조가 동결건조에 비하여 각각 18.15% 및 45.75% 높은 활성을 나타내었다. 반면 ferrous ion chelating 효과에서는 동결건조(52.36%)>적외선건조(47.16%)>열풍건조(30.04%)의 순으로 동결건조 처리구에서 높은 활성을 나타내었다. ACE 저해활성에서는 열풍건조 및 적외선건조가 동결건조에 비하여 각각 9.30% 및 30.88%가 증가하였으며, XO 저해 활성에서는 열풍건조 및 적외선건조가 동결건조에 비하여 각각 5.09% 및 9.02%가 증가하였다. 이상의 결과 적외선 건조 처리한 꾸지뽕나무 열매 추출물은 다양한 항산화 활성 증대효과를 나타냄으로써 산화적 손상 예방 소재개발에 활용가치가 있다고 사료된다.

과제정보

연구 과제 주관 기관 : 대구가톨릭대학교

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  7. Development of Quantitative Analytical Method for Isoflavonoid Compounds from Fruits of Cudrania Tricuspidata vol.44, pp.3, 2016, https://doi.org/10.5658/WOOD.2016.44.3.337