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Enzymatic Production of Alkyl β-Glucoside Based on Transglycosylation Activity of Celluclast

효소적 당전이 반응을 이용한 Alkyl β-Glucoside의 생산

  • Yong, Hwan-Ung (Dept. of Food Science and Nutrition, Hallym University) ;
  • Kim, Seonmi (Dept. of Food Science and Nutrition, Hallym University) ;
  • Shim, Jae-Hoon (Dept. of Food Science and Nutrition, Hallym University)
  • 용환웅 (한림대학교 식품영양학과) ;
  • 김선미 (한림대학교 식품영양학과) ;
  • 심재훈 (한림대학교 식품영양학과)
  • Received : 2012.05.23
  • Accepted : 2012.07.13
  • Published : 2012.10.31

Abstract

Alkyl glucosides were synthesized using the transglycosylation reaction of Celluclast, the cellulase from Trichoderma reesei, with cellobiose and various alcohols. Glucose as a by-product of the reaction was removed using the immobilized yeast system. Among the alkyl glucoside products, the acceptor products of methanol and ethanol were confirmed as methyl ${\beta}$-D-glucopyranoside and ethyl ${\beta}$-D-glucopyranoside via MALDI-TOF MS and enzymatic analysis. Optimal yields of methyl ${\beta}$-glucoside and ethyl ${\beta}$-glucoside were 65.3% (mol/mol) and 59.0% (mol/mol), respectively, based on cellobiose consumed.

Acknowledgement

Supported by : 한림대학교

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