Performance of Chlorella vulgaris for the Removal of Ammonia-Nitrogen from Wastewater

Choi, Hee-Jeong;Lee, Seung-Mok

  • 투고 : 2012.12.11
  • 심사 : 2013.07.13
  • 발행 : 2013.12.30


In the present investigation, the efficiency of Chlorella vulgaris (C. vulgaris) was evaluated for the removal of ammonia-nitrogen from wastewater. Eight different wastewater samples were prepared with varied amounts of $NH_4-N$ concentrations from 15.22 to 205.29 mg/L. Experiments were conducted at pH $7.5{\pm}0.3$, temperature $25^{\circ}C{\pm}1^{\circ}C$, light intensity $100{\mu}E/m^2/s$, and dark-light cycles of 8-16 hr continuously for 8 days. From the results, it was found that $NH_4-N$ was completely removed by C. vulgaris, when the initial concentration was between 5.22-25.24 mg/L. However, only 50% removal was obtained when the $NH_4-N$ concentration was 85.52 mg/L, which further decreased to less than 32% when the $NH_4-N$ concentration exceeded 105.43 mg/L. The further influence of nitrogen on chlorophyll was studied by various $NH_4-N$ concentrations. The maximal value of chlorophyll a (Chl a) content was found to be 19.21 mg/L for 65.79 mg/L $NH_4-N$ concentration, and the maximum specific $NH_4-N$ removal rate of 1.79 mg/mg Chl a/day was recorded at an $NH_4-N$ concentration of 85.52 mg/L. These findings demonstrate that C. vulgaris could potentially be employed for the removal of $NH_4-N$ from wastewater.


Chlorella vulgaris;Microalgae;$NH_4-N$ removal;Wastewater treatment


  1. Tam NF, Wong YS. Effect of ammonia concentrations on growth of Chlorella vulgaris and nitrogen removal from media. Bioresour. Technol. 1996;57:45-50.
  2. Aslan S, Kapdan IK. Batch kinetics of nitrogen and phosphorus removal from synthetic wastewater by algae. Ecol. Eng. 2006;28:64-70.
  3. Kim J, Lingaraju BP, Rheaume R, Lee JY, Siddiqui KF. Removal of ammonia from wastewater effluent by Chlorella vulgaris. Tsinghua Sci. Technol. 2010;15:391-396.
  4. Azov Y, Goldman JC. Free ammonia inhibition of algal photosynthesis in intensive cultures. Appl. Environ. Microbiol. 1982;43:735-739.
  5. Munoz R, Rolvering C, Guieysse B, Mattiasson B. Photosynthetically oxygenated acetonitrile biodegradation by an algal-bacterial microcosm: a pilot-scale study. Water Sci. Technol. 2005;51:261-265.
  6. Ogbonna JC, Yoshizowa H, Tanaka H. Treatment of high strength organic wastewater by a mixed culture of photosynthetic microorganisms. J. Appl. Phycol. 2000;12:277-284.
  7. Aksu Z. Application of biosorption for the removal of organic pollutants: a review. Process Biochem. 2005;40:997-1026.
  8. Becker EW. Microalgae: biotechnology and microbiology. New York: Cambridge University Press; 1994.
  9. Clesceri LS, Greenberg AE, Eaton AD. Standard methods for the examination of water and wastewater. 20th ed. Washington: American Public Health Association; 1999.
  10. de-Bashan LE, Bashan Y. Immobilized microalgae for removing pollutants: review of practical aspects. Bioresour. Technol. 2010;101:1611-1627.
  11. Valderrama LT, Del Campo CM, Rodriguez CM, de-Bashan LE, Bashan Y. Treatment of recalcitrant wastewater from ethanol and citric acid production using the microalga Chlorella vulgaris and the macrophyte Lemna minuscula. Water Res. 2002;36:4185-4192.
  12. Martinez ME, Sanchez S, Jimenez JM, El Yousfi F, Munoz L. Nitrogen and phosphorus removal from urban wastewater by the microalga Scenedesmus obliquus. Bioresour. Technol. 2000;73:263-272.
  13. Olguin EJ. Phycoremediation: key issues for cost-effective nutrient removal processes. Biotechnol. Adv. 2003;22:81-91.
  14. Shi J, Podola B, Melkonian M. Removal of nitrogen and phosphorus from wastewater using microalgae immobilized on twin layers: an experimental study. J. Appl. Phycol. 2007;19:417-423.
  15. Voltolina D, Gomez-Villa H, Correa G. Nitrogen removal and recycling by Scenedesmus obliquus in semicontinuous cultures using artificial wastewater and a simulated light and temperature cycle. Bioresour. Technol. 2005;96:359-362.
  16. Huang GL, Wang Y. Nitrate and phosphate removal by coimmobilized Chlorella pyrenoidosa and activated sludge at different pH values. Water Qual. Res. J. Can. 2003;38:541-551.
  17. Akpor OB, Muchie M. Bioremediation of polluted wastewater influent: phosphorus and nitrogen removal. Sci. Res. Essays 2010;5:3222-3230.
  18. Park J, Jin HF, Lim BR, Park KY, Lee K. Ammonia removal from anaerobic digestion effluent of livestock waste using green alga Scenedesmus sp. Bioresour. Technol. 2010;101:8649-8657.
  19. Wang B, Lan CQ. Biomass production and nitrogen and phosphorus removal by the green alga Neochloris oleoabundans in simulated wastewater and secondary municipal wastewater effluent. Bioresour. Technol. 2011;102:5639-5644.


연구 과제 주관 기관 : National Research Foundation of Korea