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톳 열수 추출물이 마우스 비장세포 증식증과 염증성 사이토카인 (IL-1β, IL-6, TNF-α) 분비에 미치는 영향

Effect of Hizikia fusiforme Water Extracts on Splenocyte Proliferation and Cytokine Production in Mice

  • 박현진 (ICAN 영양연구소) ;
  • 류혜숙 (상지대학교 보건과학대학 식품영양학과)
  • Park, Hyunjin (ICAN Nutrition Education and Research) ;
  • Ryu, Hyesook (Dept. of Food and Nutrition, College of Health Sciences, Sangji University)
  • 투고 : 2013.08.19
  • 심사 : 2013.11.23
  • 발행 : 2013.12.31

초록

본 연구에서는 천연자원 식용 해조류인 톳으로부터 톳의 면역 증강효과를 알아보고자 마우스를 이용한 생체 내(in vivo) 실험 연구를 수행하였다. 톳 열수 추출물을 4주간 격일로 0, 50, 500 mg/kg b.w.의 농도로 마우스에 경구투여한 후 비장세포 증식능과 복강 대식세포에서 분비하는 사이토카인(IL-$1{\beta}$, IL-6, TNF-${\alpha}$) 분비량에 미치는 영향을 검색한 결과 두 농도 모두 Con A나 LPS로 자극 시 대조군에 비해 높은 비장세포 증식능을 보였고, 특히 50 mg/kg b.w. 농도로 투여한 군에서 비장세포 증식능이 최대를 나타내는 것을 알 수 있었다. 또한 복강 대식세포에 의한 사이토카인 분비량을 측정한 결과 LPS 첨가 시 IL-$1{\beta}$의 분비량은 500 mg/kg b.w.의 농도군에서 가장 높은 생성량이 관찰되었다. 이는 LPS를 투여한 군과 투여하지 않은 군에서 모두 동일한 경향을 보였다. IL-6 분비량은 LPS 첨가 시 50 mg/kg b.w.의 농도 투여군에서 가장 높은 생성을 나타냈다. TNF-${\alpha}$의 경우는 50 mg/kg b.w.의 농도로 투여한 군에서 대조군보다 유의적으로 높은 수준의 TNF-${\alpha}$가 분비되었다. 이상의 본 연구에서는 50 mg/kg b.w. 톳 열수 추출물 투여군에서 비장세포 증식능과 IL-$1{\beta}$, IL-6, TNF-${\alpha}$의 분비량을 상승시키는 것을 알 수 있었다. 이처럼 톳 열수 추출물은 마우스 면역계의 조절기전에 작용하여 면역세포 활성을 직접적으로 촉진시키거나 또는 이와 관련된 다른 면역반응에 영향을 미침으로써 면역활성에 효과적으로 작용할 수 있으리라 사료된다. 앞으로 인체에 대한 유용한 검색이 중요한 의의가 있다고 생각되며, 톳에 존재하는 면역활성물질의 순수 분리 및 동정이 계속적으로 이루어져야 할 필요가 있고 작용 기전과 이용에 대한 상세한 연구가 필요할 것으로 사료된다.

참고문헌

  1. Kim JG, Noh Y, Park KH, Lee JS, Kim HJ, Kim MJ, Yoon MH, Kim JS, Heu MS. 2012. Preparation of natural seasoning using enzymatic hydrolysates from byproducts of Alaska pollock Theragra chalcogramma and sea tangle Laminaria japonica. Kor J Fish Aquat Sci 45: 545-552. https://doi.org/10.5657/KFAS.2012.0545
  2. Kim ET, Oh JH. 2009. Effect of glutathione with sea tangle extract on prevention of selenite-induced cataract formation in rats. J Korean Ophthalmol Soc 50: 1555-1562. https://doi.org/10.3341/jkos.2009.50.10.1555
  3. Mo SJ, Hong HW, Bang J, Cho KW. 2011. Optimal condition for eicosapentaenoic acid production and purification from psychrophilic marine baterium Shewanella sp. L93. Korean J Microbiol Biotechnol 39: 218-223.
  4. Kim MC, Kim JS, Harikrishnan R, Han YJ, Heo MS. 2010. Identification and antioxidant activity using electron spin resonance spectrometry of antioxidant producing marine actinomycetes Streptomyces sp. ACT-18. Kor J Microbiol Biotechnol 38: 24-31.
  5. Itoh H, Noda H, Amano H, Zhuaug C, Mizuno T, Ito H. 1993. Antitumor activity and immunological properties of marine algal polysaccharides, especially fucoidan, prepared from Sargassum thunberhii of Phaeophyceae. Anticancer Res 13: 2045-2052.
  6. Kavita K, Singh VK, Jha B. 2013. 24-Branched ${\Delta}5$ sterols from Laurencia papillosa red seaweed with antibacterial activity against human pathogenic bacteria. Microbiol Res doi:10.1016/j.micres.2013.07.002. https://doi.org/10.1016/j.micres.2013.07.002
  7. Motshakeri M, Ebrahimi M, Goh YM, Matanjun P, Mohamed S. 2013. Sargassum polycystum reduces hyperglycaemia, dyslipidaemia and oxidative stress via increasing insulin sensitivity in a rat model of type 2 diabetes. J Sci Food Agric 93: 1772-1778. https://doi.org/10.1002/jsfa.5971
  8. Gomez-Ordonez E, Jimenez-Escrig A, Ruperez P. 2012. Effect of the red seaweed Mastocarpus stellatus intake on lipid metabolism and antioxidant status in healthy Wistar rats. Food Chem 135: 806-811. https://doi.org/10.1016/j.foodchem.2012.04.138
  9. Dobashi K, Nishino T, Fujihara M, Nagumo T. 1989. Isolation and preliminary characterization of fucose-containing sulfated polysaccharides with blood-anticoagulant activity from the brown seaweed Hizikia fusiforme. Carbohydr Res 194: 315-320. https://doi.org/10.1016/0008-6215(89)85032-3
  10. Ryu HS, Kim HS. 2006. Effect of Zingiber officinale and Hizikia fusiforme water extracts on NO production in macrophage of mice. Korean J Food & Nutr 19: 327-331.
  11. Jang SE, Chyun JH. 2007. Effects of dietary calcium level and Hijikia fusiforme supplementation on bone indices and serum lipid levels in ovariectomized rats. Korean J Nutr 40: 419-427.
  12. Song HS, Eom SH, Kang YM, Choi JD, Kim YM. 2011. Enhancement of the antioxidant and anti-inflammatory activity of Hizikia fusiforme water extract by lactic acid bacteria fermentation. Kor J Fish Aquat Sci 44: 111-117. https://doi.org/10.5657/kfas.2011.44.2.111
  13. Lee SJ, Yim MJ, Kim GW, Cho SY, Choi JW. 2011. Antihyperlipidemia and antiarteriosclerosis of Hizikia fusiformis in Sprague-Dawley rats. Kor J Fish Aquat Sci 44: 443-450. https://doi.org/10.5657/KFAS.2011.0443
  14. Ryu J, Hwang HJ, Kim IH, Nam TJ. 2012. Mechanism of inhibition of HepG2 cell proliferation by a glycoprotein from Hizikia fusiformis. Kor J Fish Aquat Sci 45: 553-560. https://doi.org/10.5657/KFAS.2012.0553
  15. Kang CH, Kang SH, Boo SH, Park SY, Choi YH, Moon DO, Kim GY. 2011. Ethyl alcohol extract of Hizikia fusiforme induces caspase-dependent apoptosis in human leukemia U937 cells by generation of reactive oxygen species. Trop J Pharm Res 10: 739-746.
  16. Kim SO, Choi YH. 2010. The ethyl alcohol extract of Hizikia fusiforme inhibits matrix metalloproteinase activity and regulates tight junction related protein expression in Hep3B human hepatocarcinoma cells. J Med Food 13: 31-38. https://doi.org/10.1089/jmf.2009.1233
  17. Kim TY, Jin CY, Kim GY, Choi IW, Jeong YK, Nam TJ, Kim SK, Choi YH. 2009. Ethyl alcohol extracts of Hizikia fusiforme sensitize AGS human gastric adenocarcinoma cells to tumor necrosis factor-related apoptosis-inducing ligand-mediated apoptosis. J Med Food 12: 782-787. https://doi.org/10.1089/jmf.2008.1114
  18. Kim MJ, Lee HH, Seo MJ, Kang BW, Park JU, Kim KS, Kim GY, Joo WH, Choi YH, Cho YS, Jeong YK. 2012. Identification of 5-hydroxy-3,6,7,8,3'4'-hexamethoxyflavone from Hizikia fusiforme involved in the induction of the apoptosis mediators in human AGS carcinoma cells. J Microbiol Biotechnol 22: 1665-1672. https://doi.org/10.4014/jmb.1208.08016
  19. Kang CH, Kim MJ, Seo MJ, Choi YH, Jo WS, Lee KT, Jeong YK, Kim GY. 2013. 5-Hydroxy-3,6,7,8,3'4'-hexamethoxyflavone inhibits nitric oxide production in lipopolysaccharide-stimulated BV2 microglia via NF-${\kappa}B$ suppression and Nrf-2-dependent heme oxygenase-1 induction. Food Chem Toxicol 57: 119-125. https://doi.org/10.1016/j.fct.2013.03.019
  20. Ryu HS, Jung YH, Kim HS. 2007. Effects of Hizikia fusiforme water extracts on mouse immune cell activation. Korean J Nutr 40: 624-629.
  21. Lee YS, Kim DS, Ryu BH, Lee SH. 1992. Antitumor and immunomodulating effects of seaweeds toward sarcoma-180 cell. J Korean Soc Food Nutr 21: 544-550.
  22. Cho KJ, Lee YS, Ryu BH. 1990. Antitumor effect and immunology activity of seaweeds toward sarcoma-180. Bull Korea Fish Soc 23: 345-352.
  23. Lee HY. 1998. Macrophage activating polysaccarides from Hizikia fusiforme. MS Thesis. Korea University, Seoul, Korea. p 30-31.
  24. Lee JH, Shin SJ, Moon Y. 1998. Effect of Scutellaria baicalensis extract on the immune functions, microbial growth and mutagenicity. Korean J Immunol 20: 343-348.
  25. Miossec P. 1997. Cytokine-induced autoimmune disorders. Drug Saf 17: 93-104. https://doi.org/10.2165/00002018-199717020-00002
  26. Meydani SN. 1990. Dietary modulation of cytokine production and biologic functions. Nutr Rev 48: 361-369.
  27. Arend WP, Dayer JM. 1995. Inhibition of the production and effects of interleukin-1 and tumor necrosis factor ${\alpha}$ in rheumatoid arthritis. Arthritis Rheum 38: 151-160. https://doi.org/10.1002/art.1780380202
  28. Liu M, Li J, Kong F, Lin J, Gao Y. 1998. Induction of immunomodulating cytokines by a new polysaccharide-peptide complex from culture mycelia of Lentinus edodes. Immunopharmacology 40: 187-198. https://doi.org/10.1016/S0162-3109(98)00043-5
  29. Dinarello CA, Endres S, Meydani SN, Meydani M, Hellerstein MK. 1990. Interleukin-1, anorexia, and dietary fatty acids. Ann N Y Acad Sci 587: 332-338. https://doi.org/10.1111/j.1749-6632.1990.tb00174.x
  30. Nedwin GE, Svedersky LP, Bringman TS, Palladino MA Jr, Goeddel DV. 1985. Effect of interleukin 2, interferon-gamma, and mitogens on the production of tumor necrosis factors alpha and beta. J Immunol 135: 2492-2497.
  31. Cho SH, Yang KM, Bae B, Im S, Yu R. 1998. Effect of sea tangle intake on cytokine production in macrophage from normal and diabetic mice. J Korean Soc Food Sci Nutr 27: 952-959.
  32. Son EW, Park JH, Kim KR, Kim BO, Rhee DK, Pyo S. 1998. Effects of the administration of Bezoar Bovis on immune responses of mice. Kor J Pharmacogn 29: 48-55.
  33. Yee ST, Jeong YR, Ha MH, Kim SH, Byun MW, Jo SK. 2000. Induction of nitric oxide and TNF-${\alpha}$ by herbal plant extracts in mouse macrophages. J Korean Soc Food Sci Nutr 29: 342-348.