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Determination of β-Carotene and Retinol in Korean Noodles and Bread Products

가공 및 외식식품 중 면류 및 제빵류의 레티놀 및 베타카로틴 함량 조사

  • Shin, Jung-Ah (Dept. of Food Science and Technology, Chungnam National University) ;
  • Chun, Ji Yeon (Dept. of Food Engineering, Sunchon National University) ;
  • Lee, Junsoo (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Shin, Ki Yong (Nutrition Safety Policy Division, Ministry of Food and Drug Safety) ;
  • Lee, Soon Kyu (Nutrition Safety Policy Division, Ministry of Food and Drug Safety) ;
  • Lee, Ki-Teak (Dept. of Food Science and Technology, Chungnam National University)
  • 신정아 (충남대학교 식품공학과) ;
  • 천지연 (순천대학교 식품공학과) ;
  • 이준수 (충북대학교 식품공학과) ;
  • 신기용 (식품의약품안전처 영양안전정책과) ;
  • 이순규 (식품의약품안전처 영양안전정책과) ;
  • 이기택 (충남대학교 식품공학과)
  • Received : 2013.08.08
  • Accepted : 2013.10.10
  • Published : 2013.12.31

Abstract

The contents of ${\beta}$-carotene and retinol in processed and restaurant foods, such as Korean noodles, mandus, rice cakes and bread products, were quantified by high-performance liquid chromatography (HPLC) with UV/visible and fluorescence detector, respectively. Samples were collected from different local areas (i.e. Gangwon-do, Gyeonggi-do, Gyeongsang-do, Seoul, Jeolla-do, and Chungcheong-do). After homogenization, samples were hydrolyzed by direct alkali saponification; thereafter, fat-soluble components were extracted by a mixture of n-hexane/ethylacetate (85:15, v/v), containing 0.01% butylated hydroxytoluene (BHT). ${\beta}$-carotene and retinol contents in infant formula used as an in-house material for the analytical quality control. Among 14 Korean noodles, high contents of ${\beta}$-carotene were found in Bibim-Guksu (average 442.43 ${\mu}g/100g$) and Jjolmyeon (average 301.39 ${\mu}g/100g$). In 4 Korean mandus, the highest contents of ${\beta}$-carotene was determined in Kimchi-mandu (average 197.64 ${\mu}g/100g$), resulting in 33.3 RE of the converted vitamin A. Among 12 Korean rice cakes, Maeun-Tteokbokki and Modm-Chaltteok contained relatively high content of ${\beta}$-carotene with 205.11 and 41.33 ${\mu}g/100g$, respectively, while retinol was detected only in Maeun- Tteokbokki (1.65~10.45 ${\mu}g/100g$). In addition, among 8 bread products, 77.3 RE of pastry, 51.2 RE of buttercream- bread, and 41.4 RE of morning roll were found as the contents of the converted vitamin A.

Acknowledgement

Supported by : 식품의약품안전청

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