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Determination of Eleutherosides and β-Glucan Content from Different Parts and Cultivating Areas of A. senticosus and A. koreanum

가시오가피와 탐라오가피의 산지별 및 부위별 Eleutheroside B, E 및 β-Glucan 함량 분석

  • Kim, Young-Hyun (Dept. of Food Science and Biotechnology, Kangwon National University) ;
  • Bae, Da-Bin (Dept. of Food Science and Biotechnology, Kangwon National University) ;
  • Lee, Jong Seok (Dept. of Food Science and Biotechnology, Kangwon National University) ;
  • Park, Sun-Ok (STR Biotech Co., LTD.) ;
  • Lee, Sang-Jong (STR Biotech Co., LTD.) ;
  • Cho, Ok-Hyun (University of Massachusetts Medical School) ;
  • Lee, Ok-Hwan (Dept. of Food Science and Biotechnology, Kangwon National University)
  • 김영현 (강원대학교 식품생명공학과) ;
  • 배다빈 (강원대학교 식품생명공학과) ;
  • 이종석 (강원대학교 식품생명공학과) ;
  • 박선옥 ((주)에스티알바이오텍) ;
  • 이상종 ((주)에스티알바이오텍) ;
  • 조옥현 (메사추세츠 주립대학교 의과대학) ;
  • 이옥환 (강원대학교 식품생명공학과)
  • Received : 2013.08.16
  • Accepted : 2013.11.06
  • Published : 2013.12.31

Abstract

The present study was performed to quantitatively analyze eleutherosides (B and E) and ${\beta}$-glucan in different plant parts of three cultivars (Chungnam, Gangwon, and Jeju) of Acanthopanax senticosus and Acanthopanax koreanum using HPLC and a commercial enzyme kit. Our results showed high linearity in the calibration curves as the coefficients of correlation ($R^2$) were 0.998 (eleutheroside B) and 0.999 (eleutheroside E), respectively. Eleutheroside B and E were found in stem extracts of A. koreanum cultivated in Jeju (1,122 ${\mu}g/g$, eleutheroside B) and A. senticosus cultivated in Chungnam (2,536 ${\mu}g/g$, eleutheroside E), respectively. However, eleutheroside B was not detected in any part of A. senticosus cultivated in Chungnam. For ${\beta}$-glucan contents, stems of A. senticosus and A. koreanum showed higher than other parts. Furthermore, the ${\beta}$-glucan content in stems of A. koreanum cultivated in Gangwon was significantly higher than in those of other cultivars. These results show that the contents of eleutheroside B, E, and ${\beta}$-glucan were higher in stem extracts of A. senticosus and A. koreanum than other parts. Moreover, our results suggest that the contents of eleutheroside B, E, and ${\beta}$-glucan in A. senticosus and A. koreanum are influenced by cultivation area and the selected part.

Acknowledgement

Supported by : 중소기업청

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