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Effect of antibacterial effects of myrrh, rhatany, chamomomilla against to oral microorganisms

몰약, 라타니아, 카모밀레 등의 구강 내 병원균에 대한 항균작용

  • Baek, Han-Seung (Department of Orofacial Pain and Oral Medicine, School of Dentistry, Kyung Hee University) ;
  • Kang, Soo-Kyung (Department of Orofacial Pain and Oral Medicine, School of Dentistry, Kyung Hee University) ;
  • Auh, Q-Schick (Department of Orofacial Pain and Oral Medicine, School of Dentistry, Kyung Hee University) ;
  • Chun, Yang-Hyun (Department of Orofacial Pain and Oral Medicine, School of Dentistry, Kyung Hee University) ;
  • Hong, Jung-Pyo (Department of Orofacial Pain and Oral Medicine, School of Dentistry, Kyung Hee University)
  • 백한승 (경희대학교 치의학전문대학원 안면통증구강내과학교실) ;
  • 강수경 (경희대학교 치의학전문대학원 안면통증구강내과학교실) ;
  • 어규식 (경희대학교 치의학전문대학원 안면통증구강내과학교실) ;
  • 전양현 (경희대학교 치의학전문대학원 안면통증구강내과학교실) ;
  • 홍정표 (경희대학교 치의학전문대학원 안면통증구강내과학교실)
  • Received : 2013.10.21
  • Accepted : 2013.11.10
  • Published : 2013.12.30

Abstract

Even though there exist a lot of study about antibacterial effects and reactions of extracted materials from plant, few study exist about oral pathogenic bacteria. Therefore we tried to recognize about the suppression effect to the periodontal pathogenic bacteria and halitosis, when add some kinds of plant extracted materials, myrrh, rhatany, chamomolilla to saliva. We used Crude drug : Myrrh tincture (100mg/ml), Ratanhia tincture (100mg/ml), Chamomile tincture(100mg/ml). We inspected about the cariogenic bateriae, S. mutans GS5 and S. sobrinus 6715, periodontal pathogenic bacteria, P. gingivalis 2561, P. intermedia ATCC 25611, Candida albicans ATCC 18804, and E. feacalis ATCC 4083, then the result follow. The plant extracted material, myrrh, rhatany, chamomomilla, which have convergence effect, bacteriocidal effect and anti-inflammation effect, show an antibacterial effect and reaction to the oral pathogenic bacteria. And with treating rhatany that have the most strong antibacterial effect, through transmission electron microscopy we could see a severe morphologic change of bacteria. This means with the plant extracted material, we can suppress the oral harmful bacteria and prevent periodontal diseases, caries, halitosis and oral inflammations. And within the future studies for the improvement of oral hygiene, our result might be a clinical evidence.

References

  1. Buchbauer, G. Aromatherapy: evidence for sedative effects of the essential oil of lavender after inhalation. Z. Naturfoschc. 1991;46: 1067-1072.
  2. A Akpan, R Morgan Oral candidiasis Postgrad Med J. 2002;78:455-459. https://doi.org/10.1136/pmj.78.922.455
  3. Lamont RJ, Jenkinson HF. Life below the gum line: pathogenic mechanisms of Porphyromonas gingivalis. Microbiol Mol Biol Rev 1998;62: 1244-1263.
  4. Tanner AA, Socransky SS, Goodson JM. Microbiota of periodontal pockets losing crestal alveolar bone. J Periodontal Res 1984;11:279-291.
  5. Tamai R, Asai Y, Ogawa T. Requirement for intercellular adhesion molecule 1 and caveolae in invasion of human oral epithelial cells by Porphyromonas gingivalis. Infect Immun 2005; 73:6290-6298. https://doi.org/10.1128/IAI.73.10.6290-6298.2005
  6. Christersson LA, Rosling BG, Dunford RG et al. monitoring of subgingival Bacteroides gingivalis and Actinobacillus actinomycetemcomitans in the management of advanced periodontitis. Adv Dent Res 1988;2:382-388.
  7. Lawless J. The illustrated encyclopedia of essential oils. Shafesbury. UK. 1995. Element books Itd.
  8. Lis-Balchin M. Essential oils and aromatherapy: their modern role in healing. J R Soc Health. 1977;117:324-329.
  9. Takarada, K., Kimizuka, R., Takahashi, N., Honma, K., Okuda, K. and Kato, T. : A comparison of the antibacterial efficacies of essential oils against oral pathogens. Oral Microbiol Immunol. 2004;19:61-64. https://doi.org/10.1046/j.0902-0055.2003.00111.x
  10. Shapiro, S., Meier, A. and Guggenheim, B. The antimicrobial activity of essential oils and essential oil components towards oral bacteria. Oral Microbiol Immunol. 1994; 9:202-208. https://doi.org/10.1111/j.1399-302X.1994.tb00059.x
  11. Schoenknecht, F.D., Sabath, L.D. and Thornsberry, C. Susceptibility tests: special tests. In Manual of Clinical Microbiology, 4th Ed., Washington D.C., 1985. Lennette,. E.H., Balows, A., Hausler, W.J.Jr. and Shadomy, H.J. (eds), American Society for Microbiolgy. pp1000-1008.
  12. Alviano, W.S., Mendonca-Filho, R.R., Alviano, D.S., Bizzo, H.R., Souto-Padron, T., Rodrigues, M.L., Bolognese, A.M., Alviano, C.S. and Souza, M.M.. Antimicrobial activity of Croton cajucara Benth linalool-rich essential oil on artificial biofilms and planktonic microorganisms. Oral Microbiol Immunol. 2005;20:101-105. https://doi.org/10.1111/j.1399-302X.2004.00201.x
  13. Chung, J.Y., Choo, J.H., Lee, M.H. and Hwang, J.K.. Anticariogenic activity of macelignan isolated from Myristica fragrans (nutmeg) against Streptococcus mutans. Phytomedicine. 2006;13:261-266. https://doi.org/10.1016/j.phymed.2004.04.007
  14. 한지숙, 신동화, 백남인. 몰약(Commiphora molmol Engl.)의 식중독 미생물 증식억제 물질의 구조동정 및 식품적용. KOREAN J. Food SCI. TECHNOL. 2001;33(4):401-408.
  15. 장영아, 천순주, 장민정, 전동하, 조우아, 권오준, 김태훈, 김영훈, 이진태 : 몰약(Commiphora molmol)의 항염증 및 화장품 약리활성에 관한 연구. J. Kor. Soc. Cosm. 2009;15(3):727-734.
  16. 정세원, 김미경. 캐모마일, 세이지, 녹차 건분의 섭취가 노령흰쥐의 항산화능에 미치는 영향. 韓國營養學會誌. 2003;36(7):699-710.
  17. Rosan B, Lamont RJ. Dental plaque formation. Microbes Infect 2000;2:1599-1607. https://doi.org/10.1016/S1286-4579(00)01316-2
  18. Minah GE, Loesche WJ. Sucrose metabolism by prominent members of the flora isolated from cariogenic and non-cariogenic dental plaques. Infect Immun 1977;17:55-61.
  19. 이현옥, 백승화, 한동민. 편백정유의 항균효과, Kor. J. Appl. Microbiol. Biotechnol. 2001;29:253-257.
  20. Ghannoum MA, Radwan SS. Candida adherence to epithelial cells. Boca Raton, FL, 1990, CRC Press.
  21. Abu-Elteen KH, Abu-Alteen RM. The prevalence of candida albicans populations in the mouths of complete denture wearers. New Microbiol. 1998;21:41-48.
  22. Manning DJ, Coughlin RP, Poskit EM. Candida in mouth or on dummy? Arch Dis Child. 1985;60:381-382. https://doi.org/10.1136/adc.60.4.381
  23. Berdicevsky I, Ben-Aryeh H, Sazargel R, et al. Oral candida in children. Oral Surg Oral Med Oral Pathol. 1980;57:37-40.
  24. Lucas VS. Association of psychotropic drugs, prevalence of denture-related stomatitis and oral candidosis. Community Dent Oral Epidemiol. 1993;21:313-316. https://doi.org/10.1111/j.1600-0528.1993.tb00782.x
  25. Arendorf TM, Walker DM. The prevalence and intra-oral distribution of Candida albicans in man. Arch Oral Biol. 1980;25:1-10. https://doi.org/10.1016/0003-9969(80)90147-8
  26. Aldred MJ, Addy M, Bagg J, et al. Oral health in the terminally ill: across sectional pilot survey. Spec Care Dentist. 1991;11:59-62. https://doi.org/10.1111/j.1754-4505.1991.tb00815.x
  27. Cumming CG, Wight C, Blackwell CL, et al. Denture stomatitis in the elderly. Oral Microbiol Immunol. 1990;5:82-85. https://doi.org/10.1111/j.1399-302X.1990.tb00232.x
  28. Holbrook WP, Hjorleifsdottir DV. Occurrence of oral Candida albicans and other yeast-like fungi in edentulous patients in geriatric units in Iceland. Gerodontics. 1986;2(5):153-156.
  29. Rodu B, Carpenter JT, Jones MR. The pathogenesis and clinical significance of cytologically detectable oral candida in acute leukaemia. Cancer 62:2042-6, 1988 https://doi.org/10.1002/1097-0142(19881101)62:9<2042::AID-CNCR2820620928>3.0.CO;2-D
  30. Dupont B, Graybill JR, Armstrong D, et al. Fungal infections in AIDS patients. J Med Vet Mycol. 1992;30(1):19-28. https://doi.org/10.1080/02681219280000731
  31. Odds FC, Activity of Cilofungin (Ly121019) against Candida species in vitro. J Antimicrobial Chem. 1988;22:891-897. https://doi.org/10.1093/jac/22.6.891
  32. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev. 1999;12:564-582.
  33. Ayres H, Furr JR, Russell AD. A rapid method of evaluating permeabilizing activity against Pseudomonas aeruginosa. Lett Appl Microbiol. 1993;17:149-151. https://doi.org/10.1111/j.1472-765X.1993.tb00381.x
  34. Tanzer JM, Baranowski LK, Rogers JD, Haase EM, Scannapieco FA. Oral colonization and cariogenicity of Streptococcus gordonii in specific pathogen-free TAN:SPFOM(OM)BR rats consuming starch or sucrose diets. Arch Oral Biol. 2001;46(4):323-333. https://doi.org/10.1016/S0003-9969(00)00126-6
  35. Scannapieco FA, Haraszthy GG, Cho MI, Levine MJ. Characterization of an amylase-binding component of Streptococcus gordonii G9B. Infect Immun. 1992 ;60(11):4726-4733.
  36. Murray PA, Prakobphol A, Lee T, Hoover CI, Fisher SJ. Adherence of oral streptococci to salivary glycoproteins. Infect Immun. 1992;60 (1):31-38
  37. Tsumori H, Kuramitsu H. The role of the Streptococcus mutans glucosyltransferases in the sucrose-dependent attachment to smooth surfaces: essential role of the GtfC enzyme. Oral Microbiol Immunol. 1997;12(5):274-280. https://doi.org/10.1111/j.1399-302X.1997.tb00391.x
  38. Sano H, Shibasaki K, Matsukubo T, Takaesu Y. Effect of molecular mass and degree of deacetylation of chitosan on adsorption of Streptococcus sobrinus 6715 to saliva treated hydroxyapatite. Bull Tokyo Dent Coll. 2002; 43(2):75-82. https://doi.org/10.2209/tdcpublication.43.75
  39. Sum C, Mohanty S, Gupta PK, Kishen A. Influence of endodontic chemical treatment on Enterococcus faecalis adherence to collagen studied with laser scanning confocal microscopy and optical tweezers: a preliminary study. J Biomed Opt. 2008;13(4):044017. https://doi.org/10.1117/1.2957972
  40. Johnson JD, Chen R, Lenton PA, Zhang G, Hinrichs JE, Rudney JD. Persistence of extracrevicular bacterial reservoirs after treatment of aggressive periodontitis. J Periodontol. 2008;79(12):2305-2312. https://doi.org/10.1902/jop.2008.080254
  41. Effect of host responses on the pathogenicity of strains of Porphyromonas gingivalis. Katz J, Ward DC, Michalek SM. Oral Microbiol Immunol. 1996;11(5):309-318. https://doi.org/10.1111/j.1399-302X.1996.tb00187.x
  42. Swoboda JR, Kiyak HA, Darveau R, Persson GR. Correlates of periodontal decline and biologic markers in older adults. J Periodontol. 2008;79(10):1920-1926. https://doi.org/10.1902/jop.2008.080005
  43. Enhanced attachment of porphyromonas gingivalis to human fibroblasts mediated by Fusobacterium nucleatum. Metzger Z, Blasbalg J, Dotan M, Weiss EI. J Endod. 2009;35(1): 82-85. https://doi.org/10.1016/j.joen.2008.10.011
  44. Saito A, Inagaki S, Kimizuka R, Okuda K, Hosaka Y, Nakagawa T, Ishihara K. Fusobacterium nucleatum enhances invasion of human gingival epithelial and aortic endothelial cells by Porphyromonas gingivalis. FEMS Immuno Med Microbiol. 2008;54(3):349-355. l https://doi.org/10.1111/j.1574-695X.2008.00481.x
  45. Cox, S.D., Mann, C.M., Markham, J.L., Bell, H.C., Gustafson, J.E., Warmington, J.R. and Wyllie, S.G. : The mode of antimicrobial action of the essential oil of Melaleuca alternifolia (tea tree oil). J Appl Microbiol, 2000;88:170-175.
  46. Carson, C. F., Mee, B. J. and Riley, T. V. Mechanism of action of Melaleuca alternifolia (tea tree) oil on Staphylococcus aureus determined by time-kill, lysis, leakage, and salt tolerance assay and electron microscopy. Antimicrob Agents Chemother, 2002;46: 1914-1920. https://doi.org/10.1128/AAC.46.6.1914-1920.2002
  47. Oussalah M, Caillet S, Lacroix M. Mechanism of action of Spanish oregano, Chinese cinnamon, and savory essential oils against cell membranes and walls of Escherichia coli O157:H7 and Listeria monocytogenes. J Food Prot. 2006;69:1046-1055.
  48. Nguefack, J., Budde, B.B. and Jakobsen, M. Five essential oils from aromatic plants of Cameroon: their antibacterial activity and ability to permeabilize the cytoplasmic membrane of Listeria innocua examined by flow cytometry. Lett Appl Microbiol. 2004;39: 395-400. https://doi.org/10.1111/j.1472-765X.2004.01587.x

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