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Establishment of Pre-Harvest Residue Limit (PHRL) of Fungicides Azoxystrobin and Difenoconazole on Prunus mume fruits

매실 중 살균제 azoxystrobin과 difenoconazole의 생산단계 잔류허용기준 설정

  • Lee, Dong Yeol (Divison of Applied Life Science (BK21+ Program), Gyeongsang National University) ;
  • Kim, Yeong Jin (Dong-Eui Institute of Technology Dong-Eui Analysis Center, Dong-Eui Institute of Technology) ;
  • Park, Min Ho (Gyeongnam Provincial Office, National Agricultural Products Quality Management Service) ;
  • Lee, Seung Hwa (Gyeongnam Provincial Office, National Agricultural Products Quality Management Service) ;
  • Kim, Sang Gon (Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Kang, Nam Jun (Department of Horticulture, Gyeongsang National University) ;
  • Kang, Kyu Young (Divison of Applied Life Science (BK21+ Program), Gyeongsang National University)
  • 이동열 (경상대학교 응용생명과학부(BK21플러스 농생명산업 차세대 인재육성사업단)) ;
  • 김영진 (동의과학대학교 동의분석센터) ;
  • 박민호 (국립농산물품질관리원 경남지원) ;
  • 이승화 (국립농산물품질관리원 경남지원) ;
  • 김상곤 (경상대학교 식물생명공학연구소) ;
  • 강남준 (경상대학교 원예학과) ;
  • 강규영 (경상대학교 응용생명과학부(BK21플러스 농생명산업 차세대 인재육성사업단))
  • Received : 2013.10.23
  • Accepted : 2013.11.20
  • Published : 2013.12.31

Abstract

This study was carried out to investigate the residual characteristics of fungicide azoxystrobin and difenoconazole in Prunus mume fruits, and establish pre-harvest residue limits (PHRL) based on dissipation and biological half-lives of fungicide residues. The fungicides were sprayed onto the crop at recommended dosage once and 3 times in 7 days interval, respectively. The samples were harvested at 0, 1, 2, 4, 6, 8, 10, 12 and 14 days after treatment. These residual pesticides were extracted with QuEChERS method, clean-up with $NH_2$ SPE cartridge, and residues were analyzed by HPLC/DAD and GLC/ECD, respectively. Method quantitative limits (MQL) of azoxystrobin were 0.03 mg $kg^{-1}$ and of difenoconazole were 0.006 mg $kg^{-1}$. Average recovery were $93.2{\pm}2.49%$, $85.5{\pm}1.97%$ for azoxystrobin at fortification levels at 0.3 and 1.5 mg $kg^{-1}$, and $100.8{\pm}6.74%$, $87.6{\pm}9.92%$ for difenoconazole at fortification levels at 0.06 and 0.3 mg $kg^{-1}$, respectively. The biological half-lives of azoxystrobin were 5.9 and 5.2 days at recommended dosage once and 3 times in 7 days interval, respectively. The biological half-lives of difenoconazole were 9.3 and 8.0 days at recommended dosage once and 3 times in 7 days interval, respectively. The PHRL of azoxystrobin and difenoconazole were recommended as 5.32 and 1.64 mg $kg^{-1}$ for 10 days before harvest, respectively.

Acknowledgement

Supported by : 국립농산물품질관리원

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