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In vitro Antimutagenic and Genotoxic Effects of Sophora Radix Extracts

고삼추출물의 in vitro 항돌연변이원성과 유전독성 연구

  • Cho, Hyeon-Jo (Chemical Safety Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Yoon, Hyunjoo (Chemical Safety Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Park, Kyung-Hun (Chemical Safety Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Je-Bong (Agro-Material Safety Evaluation Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Shim, Chang-Ki (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Jin Hyo (Chemical Safety Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Jeong, Mi Hye (Agro-Material Safety Evaluation Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Oh, Jin-Ah (Chemical Safety Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Doo-Ho (Chemical Safety Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Paik, Min-Kyoung (Chemical Safety Division, National Academy of Agricultural Science, Rural Development Administration)
  • 조현조 (농촌진흥청 국립농업과학원 농산물안전성부 화학물질안전과) ;
  • 윤현주 (농촌진흥청 국립농업과학원 농산물안전성부 화학물질안전과) ;
  • 박경훈 (농촌진흥청 국립농업과학원 농산물안전성부 화학물질안전과) ;
  • 이제봉 (농촌진흥청 국립농업과학원 농산물안전성부 농자재평가과) ;
  • 심창기 (농촌진흥청 국립농업과학원 농산물안전성부 유기농업과) ;
  • 김진효 (농촌진흥청 국립농업과학원 농산물안전성부 화학물질안전과) ;
  • 정미혜 (농촌진흥청 국립농업과학원 농산물안전성부 농자재평가과) ;
  • 오진아 (농촌진흥청 국립농업과학원 농산물안전성부 화학물질안전과) ;
  • 김두호 (농촌진흥청 국립농업과학원 농산물안전성부 화학물질안전과) ;
  • 백민경 (농촌진흥청 국립농업과학원 농산물안전성부 화학물질안전과)
  • Received : 2013.10.04
  • Accepted : 2013.12.04
  • Published : 2013.12.31

Abstract

Sophorae radix extract (SRE) has been registered as an environment-friendly organic material that is widely used in the cultivation of crops in Korea. Matrine, the active ingredient in SRE, was reported as a toxic substance in the nervous system in mice. However, no information is available on its toxic effects in other organisms. Therefore, antimutagenicity and two kinds of genotoxicity tests (bacterial reverse mutation and chromosome aberration test) of two samples of SRE were investigated in this study. Antimutagenicity test was experimented by using bacterial reverse mutation test. In the reverse mutation test, Salmonella Typhimurim TA98, TA1535 and TA1537 were used to evaluate the mutagenic potential of SRE. Bacterial reverse mutation test was also performed on positive and negative control groups in the presence of the metabolic activation system (with S-9 mix) and metabolic non-activation system (without S-9 mix). In the chromosome aberration test, Chinese hamster lung cells were exposed to SRE for 6 or 24 hours without S-9 mix, or for 6 hours with S-9 mix. Negative and positive control groups were experimented for chromosome aberration test. As a result, the number of mutated colonies induced by 4-NQO were reduced by SRE treatment in all strains, indicating that SRE may have antimutagenic effects. Reverse mutation was not shown at all concentrations of SRE, regardless of application of the metabolic activation system. In the chromosomal aberration test, one of the SRE sample gave a suspicious positive result at 250 ${\mu}g/ml$ in the presence of S-9 mix. For the more adequate evaluation of the genotoxic potential of SRE samples, other in vivo genotoxicity study is needed.

Acknowledgement

Supported by : 농촌진흥청

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