DOI QR코드

DOI QR Code

Acute Ecotoxicity Evaluation of Environmental-friendly Organic Agro-materials Containing Pepper Extract, Cassia Oil, Lavender Oil for Control of Diamondbackmoth

배추좀나방 방제약제로서 후추 추출물, 카시아 오일, 라벤더 오일 함유 친환경유기농자재에 대한 급성 생태독성평가

  • You, Are-Sun (Division of Agro-material Safety Evaluation, Department of Agro-food Safety, National Academy of Agricultural Science) ;
  • Jeong, Mihye (Division of Agro-material Safety Evaluation, Department of Agro-food Safety, National Academy of Agricultural Science) ;
  • Hong, Soon-Seong (Division of Agro-material Safety Evaluation, Department of Agro-food Safety, National Academy of Agricultural Science) ;
  • Chang, Hee-Seop (Division of Agro-material Safety Evaluation, Department of Agro-food Safety, National Academy of Agricultural Science) ;
  • Lee, Je Bong (Division of Agro-material Safety Evaluation, Department of Agro-food Safety, National Academy of Agricultural Science) ;
  • Park, Kyung-Hun (Division of Agro-material Safety Evaluation, Department of Agro-food Safety, National Academy of Agricultural Science) ;
  • Lee, Young Mook (Division of Agro-material Safety Evaluation, Department of Agro-food Safety, National Academy of Agricultural Science) ;
  • Ihm, Yangbin (Division of Agro-material Safety Evaluation, Department of Agro-food Safety, National Academy of Agricultural Science)
  • 유아선 (국립농업과학원 농산물안전성부 농자재평가과) ;
  • 정미혜 (국립농업과학원 농산물안전성부 농자재평가과) ;
  • 홍순성 (국립농업과학원 농산물안전성부 농자재평가과) ;
  • 장희섭 (국립농업과학원 농산물안전성부 농자재평가과) ;
  • 이제봉 (국립농업과학원 농산물안전성부 농자재평가과) ;
  • 박경훈 (국립농업과학원 농산물안전성부 농자재평가과) ;
  • 이영묵 (국립농업과학원 농산물안전성부 농자재평가과) ;
  • 임양빈 (국립농업과학원 농산물안전성부 농자재평가과)
  • Received : 2013.11.14
  • Accepted : 2013.12.11
  • Published : 2013.12.31

Abstract

Environment-friendly agro-materials tend to be preferred to chemical insecticides recently. For this reason, many studies are conducted to develop environment-friendly insecticides containing natural materials. The purpose of this study was to assess ecotoxicity for pepper extract and cassia oil (11.5+46%, A), pepper extract and cassia oil (23+34%, B), and pepper extract and lavender oil (40+10%, C). They are expected to prevent from pests especially diamondback moth, and can be used for agro-materials. Their formulation was emusifiable concentration (EC). Target species used to assess acute toxicity were aquatic invertebrate (Daphina magna), fish (Cyprinus carpio), honeybee (Apis mellifera L.) and earthworm (Eisenia fetida). The $EC_{50}$ value of A, B, and C to aquatic invertebrate were 0.46, 1.9, 0.25 mg $L^{-1}$ respectively and these values were moderately toxic according to standard of USEPA. In case of acute toxicity test to fish, the $LC_{50}$ of A, B, and C were 1.9, 2.9, 3.8 mg $L^{-1}$ respectively. A was category II in acute toxicity of fish and not acceptable to evaluation criteria of environment-friendly agro-materials. B and C were category III and acceptable. Acute contact and oral toxicity test to honeybee were conducted and the $LD_{50}$ of A, B, and C were > 100 ${\mu}g$ a.i. $bee^{-1}$ in both of tests. It indicated they were low toxic to honeybee. In case of acute toxicity test to earthworm, $LC_{50}$ of A, B, and C were 695, 988, and 564 mg $kg^{-1}$. In conclusion, pepper extract+cassia oil 57% EC and pepper extract+lavender oil 50% EC were expected to be used for environment-friendly insecticide materials with low risk against ecosystem and contribute to developing environment-friendly agro-materials.

Acknowledgement

Supported by : 농촌진흥청

References

  1. Wink, M. (1993) Production and application of phytochemicals from an agricultural perspective. In Phytochemistry and Agriculture (van Beek TA, Breteler H. eds.). Clarendon Press, Oxford, pp. 171-213.
  2. Xu, S. X., Y. C. Li, X. Liu, L. J. Mao, H. Zhang and X. D. Zheng (2011) In vitro and in vivo antifungal activity of a water-dilutable cassia oil microemulsion against Geotrichum citri-aurantii. Journal of Science of Food and Agriculture 92(13): 2668-2671.
  3. Yoo, M. Y., Y. J. Jung and J. Y. Yang (2005) Antimicrobial activity of herb extracts. Journal of Korean Soc Food Sci Nutr. 34:1130-1135. https://doi.org/10.3746/jkfn.2005.34.8.1130
  4. Yook, C. S. (1990) An an illustrated guide to Korean medicinal plant, Academy books, Seoul. pp. 590.
  5. Yoon, H. H., Y. S. Park, J. B. Kim and T. R. Hahn (1995) Identification of anthocyanins from Korean pigmented rice. Agri. Chem. Biotech. 38:581-583.
  6. Agro-Material industry Division, RDA (2012) Creteria and method of toxicity test for environmental organism Legislation and Notification.Directive for pesticide regulation, RDA. pp. 365-372.
  7. Agro-Material industry Division, RDA (2012) Enforcement ordinace, attach 2 in legislation for pesticide regulation, Legislation and Notification.Directive for pesticide regulation, RDA. pp. 143-144.
  8. Agro-Material industry Division, RDA (2013) Legislation on promotion of environmental agriculture and fishery and regulation.support for organic food. pp. 257.
  9. Arnason, J. T., B. J. R. Philogene, P. Morand, K. Imrie, S. Iyengar, F. Duval, C. Soucy-Breau, J. V. Scaiano, N. H. Werstiuk, B. Hasspieler and A. E. R. Downe (1989) Naturally occurring and synthetic thiopenes as photoactivated insecticides. In Insecticides of Plant Orgin. (Eds. J. T. Arnason, B. J. R. Philogene and P. Morand) ACS symposium series no. 387, American Chemical Society, Washington, DC. pp. 164-172.
  10. Bak, W. C., B. H. Lee, K. H. Ka, T. S. Cho, H. J. Lee, S. S. Lee, M. K. Kim and B. Cha (2006) Control of Diatrype sitgma occurred on the bed-log of Shiitake using wood vinegar plant extracts and fungicides. The Korean Journal of Mycology, 34(1):22-28. https://doi.org/10.4489/KJM.2006.34.1.022
  11. Bang, K. H., D. W. Lee, H. M. Park and Y. H. Rhee (2000) Inhibition of fungal cell wall synthesizing enzymes by transcinnamaldehtde. Biosci. Biotech. Biochem. 64: 1061-1063. https://doi.org/10.1271/bbb.64.1061
  12. Chung, H. R., J. Y. Lee, D. C. Kim and W. I. Hwang (1999) Synergistic effect of Panax ginseng and Cinnamomum cassia Blume mixture on the inhibition of cancer cell growth in vitro. J. Ginseng Res. 23:99-104.
  13. Feng, W. and X. Zheng (2006) Control of Alternaria alternata by cassia oil in combination with potassium chloride or sodium chloride. J Appl Microbiol. 101:1317-1322. https://doi.org/10.1111/j.1365-2672.2006.03024.x
  14. Feng, W., X. Zheng, J. Chen and Y. Yang (2008) Combination of cassia oil with magnesium sulphate for control of postharvest storage rots of cherry tomatoes. Crop Protect 27:112-117. https://doi.org/10.1016/j.cropro.2007.04.014
  15. Isman, M. B. (1999) Neem and related natural products,. In eds. by F.R.
  16. Hsu, S., J. B. Lewis, J. L. Borke, B. Singh, D. P. Dickinson, G. B. Caughman, M. L Drake, A. C. Aiken, G. Huynh, B. R. Das, T. Osakie and G. S. Schuster (2001) Chemopreventive effects of green tea polyphenols correlate with reversible induction of p57 expression. Anticancer Res. 21:3743-3748.
  17. Koul, O. (2004) Neem: a global perspective. In eds. by O. Koul and S. Wahab. Neem: today and in the new millennium. Kluwer Academic Press. Dordrech. pp. 1-19.
  18. Martin, S., E. Andriambeloson, K. Takeda and R. Andriantsitohaina (2002) Red wine polyphenols increase calcium in bovine aortic endothelial cells: a basis to elucidate signalling pathways leading to nitric oxide production. Br. J. Pharmacol. 135:1579-1587. https://doi.org/10.1038/sj.bjp.0704603
  19. Mazzanti, G., L. Battinelli and G. Salvatore (1998) Antimicrobial properties of the linalool rich essential oil of Hyssopus officinalis L. var. decummbens (Lamiaceae). J. Flavour Frag. 13:289-294. https://doi.org/10.1002/(SICI)1099-1026(1998090)13:5<289::AID-FFJ750>3.0.CO;2-A
  20. Park, K. W. (2003) Herb & Aromatheraphy. Sunjimunhwasa, Seoul.
  21. Park, H. M. (2010) Plant Essential Oils and their Components: The Larvicidal Activities against Aedes aedes aegypti, Acute Toxicities on Water Flea, Daphnia magna, and Aqueous Residues.
  22. Saxena, R. C. (1989) Insecticides from neem. In insecticides of plant origin (J.T. Arnason, B.J.R. Philogene and P. Morand, eds.). ACS Symp. Ser. No. 387. Am. Chem. Soc. Washington, D.C. pp. 110-135.
  23. Schmutterer, H. (1980) Natural pesticides from the neem tree. Proc. 1st Int. Neem Conf. pp. 33-259.
  24. You, A. S., K. H. Park, M. Jeong and S. S. Hong (2011) Acute toxicity evaluation of plant extracts and materials inhibiting active subtances of insect, 2011 NAAS report of research business, RDA, 11-13908-2-000046-10. pp. 1708-1734.

Cited by

  1. Evaluation of Some Insecticides and Environmental Friendly Agricultural Materials against Winter Cherry Bug, Acanthocoris sordidus (Coreidae, Hemiptera) vol.20, pp.2, 2016, https://doi.org/10.7585/kjps.2016.20.2.159
  2. vol.61, pp.2, 2018, https://doi.org/10.3839/jabc.2018.022
  3. Acute Toxicities of Emulsifiable Concentrates and Granules of Valeriana fauriei Briquet and Alpinia galangal Swartz Essential Oils against Cyprinus carpio vol.36, pp.4, 2018, https://doi.org/10.11626/KJEB.2018.36.4.659