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Cultural Characteristics and Mechanism of Bacillus amyloliquefacien subsp. plantarum CC110 for Biological Control of Cucumber Downy Mildew

Bacillus amyloliquefaciens subsp. plantarum CC110균주의 오이 노균병 발생 억제기작 및 배양적 특성

  • Lee, Sang Yeob (Agricultural Microbiology Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA)) ;
  • Weon, Hang Yeon (Agricultural Microbiology Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA)) ;
  • Kim, Jeong Jun (Agricultural Microbiology Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA)) ;
  • Han, Ji Hee (Agricultural Microbiology Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA))
  • 이상엽 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 원항연 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 김정준 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 한지희 (농촌진흥청 국립농업과학원 농업미생물과)
  • Received : 2013.11.11
  • Accepted : 2013.12.13
  • Published : 2013.12.31

Abstract

An isolate of Bacillus amyloliquefaciens subsp. plantarum CC110 was selected as an effective antagonist for biological control of cucumber downy mildew caused by Pseudoperonospora cubensis. Temperature range for growth of CC110 isolate was $7.5{\sim}55.0^{\circ}C$, and its optimal temperature at $36.6^{\circ}C$. pH range for growth of CC110 isolate was 4.5~9.5, and its optimal pH at 7.0. In this study, the most effective sources of carbon and nitrogen for growth of CC110 isolate were fructose and yeast extract, respectively. The volatile of CC110 isolate was found to be effective to control downy mildew on cucumber showing no diseased area whereas that of control was 13.2% using the I plate bioassay. The culture broth and cells of isolate CC110 cultured in TSB media for 48 hours at $28^{\circ}C$ inhibited occurrence of cucumber downy mildew. The cells and culture broth were transformed into sporangia of P. cubensis by in observation under light microscope and scanning electron microscope.

Acknowledgement

Supported by : 국립농업과학원

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