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Characteristics and Virulence Assay of Entomopathogenic Fungus Metarhizium anisopliae for the Microbial Control of Spodoptera exigua

파밤나방의 생물적 방제를 위한 곤충병원성 곰팡이 Metarhizium anisopliae의 특성 및 병원성 검정

  • Han, Ji Hee (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Hyeonggyeong (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Leem, Hun Tae (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Jeong Jun (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Lee, SangYeob (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA)
  • 한지희 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 김형경 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 임훈태 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 김정준 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 이상엽 (농촌진흥청 국립농업과학원 농업미생물과)
  • Received : 2013.11.08
  • Accepted : 2013.12.20
  • Published : 2013.12.31

Abstract

Beet armyworm, Spodoptera exigua is difficult to control using chemical insecticides because of the fast development of insecticide resistance. For eco-friendly beet armyworm managements, various control agents are required. Entomopathogenic fungus is one of the promise control agents as an alternative to chemical control agent. We isolated entomopathogenic fungi from soil samples of Yangpyeong, Gyeonggi-do by insect-bait method using Tenebio molitor and conducted bioassay to larva of beet armyworm. The result of bioassay, a selected strain FT83 showed 100% mortality against third instar larva of S. exigua. On the basis of morphological characteristics and analysis of 18srRNA sequence for ITS, the strain FT83 was identified as a Metarhizium anisopliae. The mortality of beet armyworm showed $81.6{\pm}9.3%$ at $1{\times}10^6$ conidia/ml, 100% at $1{\times}10^7$ conidia/ml and 100% at $1{\times}10^8$ conidia/ml respectively. Therefore, we recommend to proper control efficacy against S. exigua in which more than $1{\times}10^7$ conidia/ml suspension of M. anisopliae FT83.

Acknowledgement

Supported by : 국립농업과학원

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