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Genome Sequence Analysis of Chrysanthemum White Rust pathogen Puccinia horiana and Sterol 14-demethylase as Drug Target

국화흰녹병균 Puccinia horiana 유전체 분석과 약물 표적으로서의 sterol 14-demethylase

  • Kim, Jeong-Gu (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Park, Sang Kun (Floriculture Research Division, National Institute of Horticultural & Herbal Science) ;
  • Park, Ha-Seung (Yesan Chrysanthemum Experiment Station, Chungcheongnam-do Agricultural Research and Extension Services) ;
  • Kwon, Soo-Jin (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Seung Hwan (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Dong-Jun (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Sohn, Seong-Han (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Byoung Moo (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Bae, Shin-Chul (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Ahn, Il-Pyung (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Changhoon (Macrogen Inc.) ;
  • Baek, Jeong Hun (Macrogen Inc.)
  • 김정구 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 박상근 (국립원예특작과학원 원예작물부) ;
  • 박하승 (충청남도 농업기술원 예산국화시험장) ;
  • 권수진 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 김승환 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 이동준 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 손성한 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 이병무 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 배신철 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 안일평 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 김창훈 (주식회사 마크로젠) ;
  • 백정훈 (주식회사 마크로젠)
  • Received : 2013.11.14
  • Accepted : 2013.12.16
  • Published : 2013.12.31

Abstract

Chrysanthemum is an economically important horticultural plant in many countries. The white rust is one of the most devastating diseases caused by an obligate fungal pathogen Puccinia horiana. This is being controlled mostly by application of chemicals. In Korea, 26 items are registered and 10 items contain 6 triazole compounds. To identify and to obtain the information of the drug target for triazoles, possible sterol 14-demethylase orthologues were extracted. From the draft genome information, the nucleotide sequence of the sterol 14-demethylase gene was identified. The amino acid sequence was deduced and the tertiary structure of the enzyme was predicted. This protein showed no less than 84% amino acid sequence identities to those of genus Puccinia and no more than 68% to those of other genus.

Acknowledgement

Supported by : 국립농업과학원

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