Isolation and Identification of Antioxidants from Methanol Extract of Sword Bean (Canavalia gladiata)

작두콩의 메탄올 추출물로부터 항산화 활성 화합물의 분리 및 동정

  • Kim, Jong-Pil (Health and Environment Research Institute of Gwangju) ;
  • Lee, Hyang-Hee (Health and Environment Research Institute of Gwangju) ;
  • Moon, Jae-Hak (Department of Food Science & Technology, and Functional Food Research Center, Chonnam National University) ;
  • Ha, Dong-Ryong (Health and Environment Research Institute of Gwangju) ;
  • Kim, Eun-Sun (Health and Environment Research Institute of Gwangju) ;
  • Kim, Jin-Hwan (Department of Applied Chemical Engineering, Chonnam National University) ;
  • Seo, Kye-Won (Health and Environment Research Institute of Gwangju)
  • 김종필 (광주광역시보건환경연구원) ;
  • 이향희 (광주광역시보건환경연구원) ;
  • 문제학 (전남대학교 식품공학과 및 기능성식품연구센터) ;
  • 하동룡 (광주광역시보건환경연구원) ;
  • 김은선 (광주광역시보건환경연구원) ;
  • 김진환 (전남대학교 응용화학공학부) ;
  • 서계원 (광주광역시보건환경연구원)
  • Received : 2013.06.30
  • Accepted : 2013.10.15
  • Published : 2013.12.31


The ethyl acetate (EtOAc) layer of Canavalia gladiata (sword bean) methanol extracts showed higher 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity than other layers. Four phenolic compounds were isolated from the EtOAc layer by silica gel column chromatography and prep-HPLC using a guided DPPH radical-scavenging assay. The isolated compounds were identified as methyl gallate (1), gallic acid (2), 1,6-di-O-galloyl ${\beta}$-$\small{D}$-glucopyranoside (3), and 1,4,6-tri-O-galloyl ${\beta}$-$\small{D}$-glucopyranoside (4) based on MS and NMR analyses. Among the four compounds, no. 4 was isolated from this plant for the first time. Their DPPH radical-scavenging activities based on $SC_{50}$ decreased in the following order: 4 (6.9 ${\mu}M$)>3 (8.3 ${\mu}M$)>2 (10.0 ${\mu}M$)>1 (10.3 ${\mu}M$).


Supported by : 보건환경연구원


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