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Isolation and Identification of Antioxidants from Methanol Extract of Sword Bean (Canavalia gladiata)

작두콩의 메탄올 추출물로부터 항산화 활성 화합물의 분리 및 동정

  • Received : 2013.06.30
  • Accepted : 2013.10.15
  • Published : 2013.12.31

Abstract

The ethyl acetate (EtOAc) layer of Canavalia gladiata (sword bean) methanol extracts showed higher 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity than other layers. Four phenolic compounds were isolated from the EtOAc layer by silica gel column chromatography and prep-HPLC using a guided DPPH radical-scavenging assay. The isolated compounds were identified as methyl gallate (1), gallic acid (2), 1,6-di-O-galloyl ${\beta}$-$\small{D}$-glucopyranoside (3), and 1,4,6-tri-O-galloyl ${\beta}$-$\small{D}$-glucopyranoside (4) based on MS and NMR analyses. Among the four compounds, no. 4 was isolated from this plant for the first time. Their DPPH radical-scavenging activities based on $SC_{50}$ decreased in the following order: 4 (6.9 ${\mu}M$)>3 (8.3 ${\mu}M$)>2 (10.0 ${\mu}M$)>1 (10.3 ${\mu}M$).

Keywords

Canavalia gladiata;sword bean;1,4,6-tri-O-galloyl ${\beta}$-$\small{D}$-glucopyranoside;galloyl glucoside;gallic acid derivative

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Acknowledgement

Supported by : 보건환경연구원