Antibacterial Effect of Huanggeumjakyak-tang against Methicillin-resistant Staphylococcus aureus

황금작약탕(黃芩芍藥湯)의 MRSA에 대한 항균활성에 관한 연구

  • Kim, Esther (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • Choi, Chonghwan (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • Kim, Ilhyun (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • Lee, Hail (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • Song, Yungsun (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University)
  • 김에스더 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 최종환 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 김일현 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 이하일 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 송용선 (원광대학교 한의과대학 한방재활의학과교실)
  • Published : 2013.07.31


Objectives The aim of this study was to investigate antimicrobial activity of Huanggeumjakyak- tang water extract against MRSA. Methods The antibacterial activities of Huanggeumjakyak-tang were evaluated against 3 strains of Methicillin-resistant Staphylococcus aureus (MRSA) and 1 standard Methicillinsusceptible S. aureus (MSSA) strain by using the disc diffusion method, minimal inhibitory concentrations (MICs) assay, colorimetric assay using MTT test, checkerboard dilution test and time-kill assay was performed under dark. Results The MIC (minimum inhibitory concentration) of Huanggeumjakyak-tang water extract against S. aureus strains ranged from 1,000 to $2,000{\mu}g/ml$. So we confirmed that it has a strong antibacterial effect. Also the combinations of Huanggeumjakyak-tang water extract and conventional antibiotics exhibited improved inhibition of MRSA with synergy effect. Conclusions The results obtained in this study suggest that Huanggeumjakyak-tang water extract showed antibacterial effect against MRSA, and it also showed reducing effect on the side-effect problems that are the major weak points of traditional antibiotics.


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