레스베라트롤의 세포보호 및 항산화 효과

DOI QR코드

DOI QR Code

조나래;박수아;전소하;하지훈;박수남
Jo, Na Rae;Park, Su Ah;Jeon, So Ha;Ha, Ji Hoon;Park, Soo Nam

  • 발행 : 2013.10.31

초록

본 연구에서는, 레스베라트롤의 산화적 손상대한 세포 보호 효과와 항산화능을 조사하였다. 레스베라트롤의 Free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) 소거활성은 ($FSC_{50}$) $103{\mu}M$로 나타났다. 루미놀화학발광법을 이용하여 $Fe^{3+}-EDTA/H_2O_2$계에서 레스베라트롤의 활성산소(Reactive oxygen species, ROS) 소거활성은($OSC_{50}$)을 평가하였다. 레스베라트롤은 $0.042{\mu}M$로 나타나 비교물질로 사용한 L-ascorbic acid ($0.405{\mu}M$)보다 9.6배 우수한 활성산소 소거활성을 보였다. 또한 레스베라트롤은 지용성 항산화제로 알려진 (+)-${\alpha}$-tocopherol보다 우수한 세포보호 활성을 나타내었다. 세포독성 조사에서 레스베라트롤은 $50{\mu}M$에서 세포독성을 나타내지 않았다. HaCaT 세포에 $800mJ/cm^2$의 UVB 또는 $30{\mu}M$의 rose-bengal을 처리하였을 때, 레스베라트롤은 농도의존적으로 세포의 산화적 손상에 대항하여 세포를 보호하였다. 그러나 10 mM의 $H_2O_2$를 처리한 경우에, 레스베라트롤은 세포보호 효과를 나타내지 않았다. 이들의 결과들은 레스베라트롤이 항산화능 및 세포보호 효과를 통해서 피부 노화를 예방하고 개선하는데 있어 이용 가능함을 시사한다.

키워드

resveratrol;antioxidative activity;cellular protective effect;oxidative damage

참고문헌

  1. J. R. Kanofsky, H. Hoogland, R. Wever, and S. J. Weiss, Singlet Oxygen Production by Human Eosinophils, J. Biol. Chem., 263, 9692 (1988).
  2. A. Oikarinen, J. Karvonen, J. Uitto, and M. Hannuksela, UVA Effects on Mammalian Skin and Cells, Photodermatology, 2, 15 (1985).
  3. A. Oikarinen and M. Kallioinen, A Biochemical and Immunohistochemical Study of Collagen in Sun-exposed and Protected Skin., Photodermatology, 6, 24 (1989).
  4. L. H. Kilgman, UVA-induced Biochemical Changes in Hairless Mouse Skin Collagen : A Contrast to UVB Effects. Ed. F. Urbach, In Biological responses to Ultraviolet A Radiation, 209, Valdemar, Overland Park (1992).
  5. J. A. Baur and D. A. Sinclair, Resveratrol Improves Health and Survival of Mice on a High-calorie Diet, Nat. Rev. Drug Discov., 5, 493 (2006). https://doi.org/10.1038/nrd2060
  6. M. M. Y. Chan, Antimicrobial Effect of Resveratrol on Dermatophytes and Bacterial Pathogens of the Skin, Biochem. Pharmacol., 63, 99 (2002). https://doi.org/10.1016/S0006-2952(01)00886-3
  7. L. Frkmont, Cranberry Flavonoids, Atherosclerosis and Cardiovascular Health, Life Sciences, 66, 663 (2000). https://doi.org/10.1016/S0024-3205(99)00410-5
  8. C. A. De la Lastra and I. Villegas, Dietary Supplementation of Resveratrol Attenuates Chronic Colonic Inflammation in Mice, Mol. Nutr. Food Res., 49, 405 (2005). https://doi.org/10.1002/mnfr.200500022
  9. F. Wolter and J. Stein, Oxyresveratrol and Resveratrol are Potent Antioxidants and Free radical Scavengers : Effect on Nitrosative and Oxidative Stress Derived from Microglial Cells, Drugs Fut., 27, 949 (2002). https://doi.org/10.1358/dof.2002.027.10.856987
  10. J. J. Docherty, J. S. Smith, M. M. Fu, T. Stoner, and T. Booth, Effect of Topically Applied Resveratrol on Cutaneous herpes Simplex virus Infections in Hairless Mice, Antiviral Res., 61, 19 (2004). https://doi.org/10.1016/j.antiviral.2003.07.001
  11. I. Kolouchova, K. Melzoch, J. Smidrkal, and V. Filip, Antioxidant Properties of Resveratrol : a Structure-Activity Insight, Chemicke Listy, 99, 492 (2005).
  12. Y. Fujita, L. Uehara, Y. Morimoto, M. Nakashima, T. Hatano, and T. Okuda, Hydrolyzable Tannins and Related Polyphenols, Yakuagaku Zasshi, 108, 129 (1988).
  13. I. Gulcin, Detection of Caraway and Bay Leaves Irradiation Based on Their Extracts' Antioxidant Properties Evaluation, Innova. Food Sci. Emerg. Tech., 11, 210 (2010). https://doi.org/10.1016/j.ifset.2009.07.002
  14. J. J. Leyden, Photoaging/photodamage and Photoprotection, Br. J. Dermatol., 122, 1 (1990).
  15. E. F. Bernstein, D. B. Brown, and F. Urback, Ultraviolet Radiation Activates the Human Elastin Promoter in Transgenic Mice : A Novel in vivo and in vitro Model of Cutaneous Photoaging, J. Investig. Dermatol., 105, 269 (1995). https://doi.org/10.1111/1523-1747.ep12318419
  16. F. Afaq, V. M. Adhami, and H. Mukhtar, Photochemoprevention of Ultraviolet B Signaling and Photocarcinogenesis, Mutat. Res., 571, 153 (2005). https://doi.org/10.1016/j.mrfmmm.2004.07.019
  17. M. A. Bachelor and G. T. Bowden, UVA-mediated Activation of Signaling Pathways Involved in Skin Tumor Promotion and Progression, Cancer Biol., 14, 131 (2004). https://doi.org/10.1016/j.semcancer.2003.09.017
  18. Y. Wan, A. Belt, and Z. Wang, UV-induced NF-${\kappa}B$ Activation and Expression of IL-6 is Attenuated by (-)-epigallocatechin-3-gallate in Cultured Human Keratinocytes in vitro, Int. J. Mol. Med., 7, 329 (2001).
  19. M. Wlaschek, G. Heinen, and A. Poswig, UVA-Induced Autocrine Stimulation of Fibroblast-derived Collagenase/MMP-1 by Interrelated Loops Of interleukin-1 and Interleukin-6, Photochem. Photobiol., 59, 550 (1994). https://doi.org/10.1111/j.1751-1097.1994.tb02982.x
  20. F. Liebel, S. Kaur, E. Ruvolo, N. Kollias, and M. D. Southall, Irradiation of Skin with Visible Light Induces Reactive Oxygen Species and Matrix-degrading Enzymes, J. Investig. Dermatol., 132, 1901 (2012). https://doi.org/10.1038/jid.2011.476
  21. J. Pincemail, Free radical and Antioxidants in Human Diseases. ed. A. E. Favier, J. Cadet, B. Kalyanaraman, M. Fontecave and J-L. Pierre, In Analysis of Free radicals in Biology Systems, 83, Birkhauser Verlag, Basel, Switzerland (1995).