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Antioxidant and skin whitening effects of Inonotus obliquus methanol extract

차가버섯 메탄올 추출물의 항산화 및 미백효과

  • 국민희 (인천산곡고등학교) ;
  • 김동하 (인천산곡고등학교) ;
  • 이찬 (인천산곡고등학교) ;
  • 정은선 (인천산곡고등학교) ;
  • 최은재 (인천산곡고등학교) ;
  • 이재성 (인천대학교 생명과학기술대학 생명과학부) ;
  • 이태수 (인천대학교 생명과학기술대학 생명과학부)
  • Received : 2013.06.11
  • Accepted : 2013.06.20
  • Published : 2013.06.30

Abstract

This study was initiated to investigate the skin whitening activities of methanol extracts from fruiting bodies of I. obliquus. The total polyphenols and flavonoids contents of I. obliquus methanol extracts were 31.85 mg/g and 28.33 mg/g, respectively. The methanol extract of the mushroom treated on B16/F10 melanoma and NIH3T3 cell lines did not show cytotoxic activity. 2,2-diphenyl-1-picrylhydrazyl(DPPH) free radical scavenging activity and chelating activity on ferrous ions of I. obliquus methanol extract were lower than those of positive control, tocopherol and BHT. The tyrosinase and L-DOPA inhibitory activities of the extract were lower than those of positive control, kojic acid and ascorbic acid. The tyrosinase and melanin synthesis inhibitory activities of the melanoma cells treated with the extract were comparable with positive control, arbutin. The experimental results suggested that methanol extract of I. obliquus contained inhibitory activities of tyrosinase and melanin synthesis in the B16/F10 melanoma cells by dose dependent manner. High ultra-violet absorption spectra in the range of 280-350 nm showed that I. obliquus extract could protect skin from UV radiation damage. Therefore, fruiting bodies of I. obliquus can be used for developing skin whitening, anti-UV and skin care agents.

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