DOI QR코드

DOI QR Code

Production of lactic acid by Lactobacillus paracasei isolated from button mushroom bed

  • Kim, Sun-Joong (Department of Bio-Environmental Chemistry, Chungnam National University) ;
  • Seo, Hye-Kyung (Department of Bio-Environmental Chemistry, Chungnam National University) ;
  • Kong, Won-Sik (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Yoon, Min-Ho (Department of Bio-Environmental Chemistry, Chungnam National University)
  • Received : 2013.11.18
  • Accepted : 2013.12.31
  • Published : 2013.12.31

Abstract

A galactose fermentation bacterium producing lactose from red seaweed, which was known well to compromise the galactose as main reducing sugar, was isolated from button mushroom bed in Buyeo-Gun, Chungchugnamdo province. The lactic acid bacteria MONGB-2 was identified as Lactobacillus paracasei subsp. tolerans by analysis of 16S rRNA gene sequence. When the production of lactic acid and acetic acid by L. paracasei MONGB-2 was investigated by HPLC analysis with various carbohydrates, the strain MONGB-2 efficiently convert the glucose and galactose to lactic acid with the yield of 18.86 g/L and 18.23 g/L, respectively and the ratio of lactic acid to total organic acids was 1.0 and 0.91 g/g for both substrates. However, in the case of acetic acid fermentation, other carbohydrates besides galactose and red seaweed hydrolysate could not be totally utilized as carbon sources for acetic acid production by the strain. The lactic acid production from glucose and galactose in the fermentation time courses was gradually enhanced upto 60 h fermentation and the maximal concentration reached to be 16-18 g/L from both substrates after 48 h of fermentation. The initial concentration of glucose and galactose were completely consumed within 36 h of fermentation, of which the growth of cell also was maximum level. In addition, the bioconversion of lactic acid from the red seaweed hydrolysate by L. paracasei MONGB-2 appeared to be about 20% levels of the initial substrates concentration and this results were entirely lower than those of galactose and glucose showed about 60% of conversion. The apparent results showed that L. paracasei MONGB-2 could produce the lactic acid with glucose as well as galactose by the homofermentation through EMP pathway.

References

  1. Datta, R., Tsai, S.P., Bonsignore, P., Moon, S.H. and Frank, J.R. 1995. Technological and economic-potential of poly(lactic acid) and lactic acid derivatives. FEMS Microbiol. Rev. 16 : 221-231. https://doi.org/10.1111/j.1574-6976.1995.tb00168.x
  2. Gao, M.T., Koide, M., Gotou, R., Takanashi, H., Hirata, M. and Hano, T. 2005. Development of a continuous electrodialysis fermentation system for production of lactic acid by Lactobacillus rhamnosus, Process Biochem. 40 : 1033-1036. https://doi.org/10.1016/j.procbio.2004.02.028
  3. Garde, A., Jonsson, G., Schmidt, A.S. and Ahring, B.K. 2002. Lactic acid production from wheat straw hemicellulose hydrolysate by Lactobacillus pentosus and Lactobacillus brevis. Bioresour. Technol. 81 : 217-223. https://doi.org/10.1016/S0960-8524(01)00135-3
  4. Hofvendahl, K. and Hahn-Hagerdal, B. 1997. L-Lactic acid production from whole wheat flour hydrolyzate using strains of Lactobacilli and Lactococci, Enzyme Microb. Technol. 20 : 301-307. https://doi.org/10.1016/S0141-0229(97)83489-8
  5. Jang, S. S., Shrai, Y., Uchida, M. and Wakisaka, M. 2012. Production of mono sugar from acid hydrolysis of seaweed. African J. Biotechnol. 11 : 1953-1963.
  6. Jang, S.S., Shrai, Y., Uchida, M. and Wakisaka, M. 2013. Potential use of Gelidium amansii acid hydrolysate for lactic acid production by Lactobacillus rhamnosus. Food Technol. Biotechnol. 51 : 131-136.
  7. Kumar, S., K., Tamura, I. Jakobsen, B., and Nei, M. 2001. MEGA2: molecular evolutionary genetics analysis software. Bioinformatics. 17 : 1244-1245. https://doi.org/10.1093/bioinformatics/17.12.1244
  8. Lunt, J. 1998. Large-scale production, properties and commercial applications of polylactic acid polymers. Polym. Degrad. Stabil. 59 : 145-152. https://doi.org/10.1016/S0141-3910(97)00148-1
  9. Miller, G. L. 1954. Use of dinitrosalysilic acid reagent for determination of reducing sugar. Anal. Chem. 31:426-428.
  10. Oh, H., Wee, Y. J., Yun, J. S., Han, S. H., Jung, S. W. and Ryu, H. W. 2005. Lactic acid production from agricultural resources as cheap raw materials. Bioresour. Technol. 96 : 1492-1498. https://doi.org/10.1016/j.biortech.2004.11.020
  11. Passos, F.V., Fleming, H.P., Ollis, D.F., Felder, R.M. and Mcfeeters, R.F. 1994. Kinetics and modeling of lactic-acid production by Lactobacillus plantarum. Appl. Environ. Microbiol. 60 : 2627-2636.
  12. Roesijadi, G., Jones, S.B., Snowden-Swan, L.J., and Zhu, Y. 2010. Macroalgae as a biomass feedstock: A Preliminary Analysis, PNNL 19944.
  13. Ryu, H.W., Yun, J.S. and Wee, Y.J. 2003. Lactic acid, pp. 635. In: Pandey, A.(Ed.), Concise Encyclopedia of Bioresource Technology. The Haworth press: New York, The USA.
  14. Saitou, N. and Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4 : 406-425.
  15. Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., and Higgins, D.G. 1997. The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25 : 4876-4882. https://doi.org/10.1093/nar/25.24.4876
  16. Uchida, M. 2005. Studies on lactic acid fermentation of seaweed. Bull. Fish. Res. Agency. 14 : 21-85
  17. Varadarajan, S. and Miller, D.J. 1999. Catalytic upgrading of fermentation-derived organic acids. Biotechnol. Prog. 15 : 845-854. https://doi.org/10.1021/bp9900965
  18. Yun, J.S. and Ryu, H.W. 2001. Lactic acid production and carbon catabolite repression from single and mixed sugars using Enterococcus faecalis RKY1. Proc. Biochem. 37 : 235-240. https://doi.org/10.1016/S0032-9592(01)00205-9