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Effect on Testosterone Production in Sprague-Dawley Rats of Gastrodia and Bee Pollen

Hong, In-Pyo;Woo, Soon-Ok;Jang, Jae-Seon

  • Received : 2012.07.31
  • Accepted : 2013.01.07
  • Published : 2013.02.28

Abstract

Objectives: We investigated the effects of Korean gastrodia and bee pollen on testosterone production in Sprague-Dawley rats. Methods: This study measured the body weight, consumption of food and water, and serum testosterone concentration in SD rats fed for four weeks with gastrodia and bee pollen. Results: The average body weights after four weeks were $375{\pm}2g$ for the control group, $358{\pm}3g$ for gastrodia consumption, and $354{\pm}2g$ and $367{\pm}8g$ for acorn pollen and Siberian gooseberry pollen, respectively. The average food consumption in SD rats over the four weeks in the control was 23.6 g for gastrodia consumption, 24.3 g and 24.9 g for acorn pollen and Siberian gooseberry pollen, respectively. The average water consumption over the four weeks was 38.33 ml in the control group, 35.0 ml for gastrodia treatment, and 33.3 ml and 33.3 ml for acorn pollen and Siberian gooseberry pollen treatment, respectively. The testosterone secretion levels in the serum of male rats were increased by gastrodia, acorn pollen, and Siberian gooseberry pollen (2.68, 3.00, and 3.28 pg/ml, respectively) compared to the control group (2.23 pg/ml). Conclusions: These results suggest that gastrodia or bee pollen may be developed as a complementary medicine to improve sexual hormone production.

Keywords

gastrodia;acorn pollen;siberian gooseberry pollen;testosterone

References

  1. Roscoe WA, Barr KJ, Mhawi AA, Pomerantz DK, Kidder GM. Failure of spermatogenesis in mice lacking connexin43.Biol Reprod. 1994; 829-838.
  2. Huff DS, Snyder III HM, Rusnack SL, Zderic SA, Carr MC, Canning DA. Hormonal therapy for the subfertility of cryptorchidism. Horm Res. 2001; 38-40.
  3. Saez JM. Leydig cells: endocrine, paracrine and autocrine regulation. Endocr Rev. 1994; 574-626.
  4. Sinclair S. Male infertility: nutritional and environmental considerations. Altern Med Rev. 2000; 28-38.
  5. Crimmel AS, Conner CS, Monga M. Withered Yang: a review of traditional Chinese medical treatment of male infertility and erectile dysfunction. J Androl. 2001; 173-182.
  6. Ryu KS, Lee HS, Kim KY, Kim MJ, Lee KG. Testosterone secretion effect according to the growth stage of silkworm (Bombyx mori L.). Int J Indust Entomol. 2010; 20: 75-77.
  7. Ryu KS, Lee HS, Kang PD, Kim KY, Kim MJ, Oh HG, Kang PD. Secretion effect of estrogen of different growth stages of silkworm (Bombyx mori L.). Int J Indust Entomol. 2011; 22: 17-20. https://doi.org/10.7852/ijie.2011.22.1.17
  8. Hong IP, Choi YS, Woo SO, Han SM, Kim HK, Lee MR, Nam SH, Ha NG. Stimulatory effect of Cordyceps militaris on testosterone production in male mouse. Kor J Mycol. 2011; 39: 148-150. https://doi.org/10.4489/KJM.2010.39.2.148
  9. Hsu CC, Huang SJ, Tsai CC, Huang BM. In vivo and in vitro stimulatory effects of Cordyceps sinensis on testosterone production in mouse Leydig cells. Life Sci. 2003; 73: 2127-2136 https://doi.org/10.1016/S0024-3205(03)00595-2
  10. Silva TMS, Camara CA, Lins ACS, Barbosa-Filho JM, Silva EMS, Freitas BM. Chemical composition and free radical scavenging activity of pollen loads from stingless bee Melipona subnitida Ducke. J Food Compos Anal. 2006; 19: 507-511. https://doi.org/10.1016/j.jfca.2005.12.011
  11. Liu CL, Liu MC, Zhu PL. Determination of Gastrodin, p-Hydroxybenzyl Alcohol,Vanillyl Alcohol, p-Hydroxylbenzaldehyde and Vanillin in Tall Gastrodia Tuber by High-Performance Liquid Chromatography. Chromatographla, 2002; 55: 317-320. https://doi.org/10.1007/BF02491665
  12. Tomas-Lorente F, Garcia-Grau MM, Nieto JL, Tomas-Barberan, FA. Flavonoids from Cistus- Ladanifer bee pollen. Phytochemistry 1992; 1: 2027-2029.
  13. Ukiya M, Akihisa T, Tokuda H, Koike K, Takayasu J, Okuda H. Isolation, structural elucidation, and inhibitory effects of terpenoid and lipid constituents from sunflower pollen on Epstein-Barr virus early antigen induced by tumor promoter,(.) TPA. J Agric Food Chem. 2003; 51: 2949-2957. https://doi.org/10.1021/jf0211231
  14. Sheen L and Chen E. Antidepressant effects of Gastrodia elata Bl. at the genomic level. BMC Complementary and Alternative Medicine. 2012; 12(1): 35 https://doi.org/10.1186/1472-6882-12-35
  15. Melita SK, Melita T, Dragutin K. Sex hormones and corticosteroids in pollen of Pinus nigra. Phytochemistry. 1979; 18(2): 345-346. https://doi.org/10.1016/0031-9422(79)80098-9
  16. Bahmanpour S, Talaei T, Vojdani Z, Panjehshahin MR, Poostpasand A, Zareei S, Ghaeminia M. Effect of Phoenix Dactylifera Pollen on Sperm Parameters and Reproductive system of Adult Male Rats. Iran J Med Sci. 2006; 31(4): 208-212.