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Effect of Different Light Emitting Diode (LED) Lights on the Growth Characteristics and the Phytochemical Production of Strawberry Fruits during Cultivation

파장별 LED광이 딸기의 생장 특성과 생리 활성 물질 형성에 미치는 효과

  • Received : 2012.05.24
  • Accepted : 2012.10.31
  • Published : 2013.02.28

Abstract

Recent unusual weather due to global warming causes shortage of daily sunlight and constitutes one of the primary reasons for agricultural damages. LED light sources are frequently utilized to compensate for the shortage of sunlight in greenhouse agriculture. The present study is aimed at evaluating formations of phytochemicals as well as growth characteristics of mature strawberry fruits ('Daewang' cultivar) during cultivation in a closed growth chamber equipped with artificial LED light as a sole light source. Each LED light of blue (448 nm), red (634 and 661 nm) or mixed blue plus red (blue:red = 3:7) was separately supplied and the intensity of each light was adjusted to $200{\pm}1{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ at plant level with a photoperiod consisted of 16 hours light and 8 hours darkness. Strawberries grown under mixed LED light of blue and red wavelengths showed a higher production of fruits than those grown under other LED treatments. Fructose, one of the free sugars, increased in mixed LED light-grown fruits. Anthocyanin contents were elevated remarkably in the mixed LED light-grown fruits compared with those in other LED treatments. Contrastingly, contents of total phenolics and flavonoids were not of much different from one another among the fruits treated with various LED lights. On the other hand, ripening of strawberry fruits was found to be faster when grown under blue LED light compared with other LED treatments. Moreover, antioxidant activities of blue or red LED light-grown fruits, respectively, were significantly higher than those of mixed LED light-grown fruits. We suggest that when daylight is in shortage during cultivation in a greenhouse, supplementation of sunlight with LED light, which is composed of blue and red wavelengths, could be useful for the enhancement of productivity as well as of free sugar content in strawberry fruits. In addition, for the strawberry culture in the plant factory, selective adoption of LED light wavelength would be required to accomplish the purpose of controlling fruit maturation time as well as of enhancing contents of sugars and antioxidants of fruits.

Keywords

anthocyanin;antioxidants;flavonoids;Fragaria ${\times}$ ananassa Duch.;sugars

References

  1. Andersen, O.M., T. Fossen, K. Torskangerpoll, A. Fossen, and U. Hauge. 2004. Anthocyanin from strawberry (Fragaria ananassa) with the novel aglycone, 5-carboxypyranopelargonidin. Phytochemistry 65:405-410. https://doi.org/10.1016/j.phytochem.2003.10.014
  2. Azad, M.O.K., I.J. Chun, J.H. Jeong, S.T. Kwon, and J.M. Hwang. 2011. Response of the growth characteristics and phytochemical contents of pepper (Capsicum annuum L.) seedling with supplemental LED light in glass house. J. Bio- Environ. Con. 23:182-188.
  3. Benson, E.E., P.T. Lynch, and J. Jones. 1992. Variation in free-radical damage in rice cell suspensions with different embryogenic potentials. Planta 188:296-305.
  4. Blois, M.S. 1958. Antioxidant determination by the use of a stable free radical. Nature 81:1199-1200.
  5. Brown, C.S., A.C. Schuerger, and J.C. Sager. 1995. Growth and photomorphogenesis of pepper plants under red light-emitting diodes with supplemental blue or far-red lighting. J. Amer. Soc. Hort. Sci. 120:808-813.
  6. Cheel, J., C. Theoduloz, J.A. Rodriguez, P.D.S. Caligari, and G. Schmeda-Hirschmann. 2007. Free radical scavenging activity and phenolic content in achenes and thalamus from Fragaria chiloensis ssp. chiloensis, F. vesca and F. ${\times}$ ananassa cv. Cehandler. Food Chem. 102:36-44. https://doi.org/10.1016/j.foodchem.2006.04.036
  7. Duong, T.N., T. Takamura, H. Watanabe, K. Okamoto, and M. Tanaka. 2003. Responses of strawberry plantlets cultured in vitro under superbright red and blue light-emitting diodes (LEDs). Plant Cell, Tissue Organ Cult. 73:43-52. https://doi.org/10.1023/A:1022638508007
  8. Ebisawa, M., K. Shoji, M. Kato, K. Shimomura, F. Goto, and T. Yoshihara. 2008. Supplementary ultraviolet radiation B together with blue light at night increased quercetin content and flavonol synthase gene expression in leaf lettuce (Lactuca sativa. L.). Environ. Control Biol. 46:1-11.
  9. Fan, L., C. Dube, C. Fang, D. Roussel, M.T. Charles, Y. Desjardins, and S. Khanizadeh. 2012. Effect of production systems on phenolic composition and oxygen radical absorbance capacity of 'Orleans' strawberry. LWT - Food Sci. Technol. 45:241-245. https://doi.org/10.1016/j.lwt.2011.09.004
  10. Folta, K.M. and K.S. Childers. 2008. Light as a growth regulator: Controlling plant biology with narrow band width solid-state lighting systems. HortScience 43:1957-1964.
  11. Gil, M.I., D.M. Holcroft, and A.A Kader. 1997. Changes in strawberry anthocyanins and other polyphenols in response to carbon dioxide treatments. J. Agric. Food Chem. 45:1662-1667. https://doi.org/10.1021/jf960675e
  12. Gill, S.S. and N. Tureja. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol. Biochem. 48:909-930. https://doi.org/10.1016/j.plaphy.2010.08.016
  13. Hernandez, I., L. Alegre, F. Van Breusegem, and S. Munne-Bosch. 2009. How relevant are flavonoids as antioxidants in plants? Trends Plant Sci. 14:125-132. https://doi.org/10.1016/j.tplants.2008.12.003
  14. Johkan, M., K. Shoji, F. Goto, S.N. Hashiad, and T. Yoshihara. 2010. Blue light-emitting diode light irradiation of seedlings improves seedling quality and growth after transplanting in red leaf lettuce. HortScience 45:1809-1814.
  15. Johkan, M., K. Shoji, F. Goto, S. Hahiad, and T. Yoshihara. 2012. Effect of green light wavelength and intensity on photomorphogenesis and photosynthesis in Lactuca sativa. Environ. Exp. Bot. 75:128-133. https://doi.org/10.1016/j.envexpbot.2011.08.010
  16. Kim, S.K., R.N. Bae, and C.H. Chun. 2011. Changes in bioactive compounds contents of 'Maehyang' and 'Seolhyang' strawberry fruits by UV light illumination. Kor. J. Hort. Sci. Technol. 29:172-180.
  17. Kim, Y.H., and M. Hamayun, A.L. Khan, C.I. Na, S.M. Kang, H.H. Han, and I.J. Lee. 2009. Exogenous application of plant growth regulators increased the total flavonoid content in Taraxacum officinale Wigg. African J. Biotechnol. 8:5727-5732.
  18. Meyers, K.J., C.B. Watkins, M.P. Pritts, and R.H. Liu. 2003. Antioidant and antiproliferative activities of strawberries. J. Agri. Food Chem. 51:6887-6892. https://doi.org/10.1021/jf034506n
  19. Moing, A., C. Renaud, M. Gaudillĕre, P. Raymond, P. Roudeillac, and B. Denoyes-Rothan. 2001. Biochemical changes during fruit development of four strawberry cultivars. J. Amer. Soc. Hort. Sci. 126:394-403.
  20. Re, R., N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, and C.R. Evans. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26:1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  21. Samuoliene, G., R. Sirtautas, A. Brazaityte, and P. Duchovskis. 2012. LED lighting and seasonality effects antioxidant properties of baby leaf lettuce. Food Chem. 134:1494-1499. https://doi.org/10.1016/j.foodchem.2012.03.061
  22. Slinkard, K. and V.L. Singleton. 1977. Total phenol analysis: Automation and comparison with manual methods. Am. J. Enol. Vitic. 28:49-55.
  23. Treutter, D. 2010. Managing phenol contents in crop plants by phytochemical farming and breeding visions and constraints. Int. J. Mol. Sci. 11:807-857. https://doi.org/10.3390/ijms11030807
  24. Wang, S.Y. and W. Zheng. 2001. Effect of plant growth temperature on antioxidant capacity in strawberry. J. Agric. Food Chem. 49:4977-4982. https://doi.org/10.1021/jf0106244
  25. Wang, S.Y. and P. Millner. 2009. Effect of different cultural systems on antioxidant capacity, phenolic content, and fruit quality of strawberries (Fragaria ${\times}$ ananassa Duch.). J. Agric. Food Chem. 57:9651-9657. https://doi.org/10.1021/jf9020575
  26. Wang, S.Y., C.T. Chen, and C.Y. Wang. 2009. The influence of light and maturity on fruit quality and flavonoid content of red raspberries. Food Chem. 112:676-684. https://doi.org/10.1016/j.foodchem.2008.06.032
  27. Wu, M.C., C.Y. Hou, C.M. Jiang, Y.T. Wang, C.Y. Wang, H.H. Chen, and H.M. Chang. 2007. A novel approach of LED light radiation improves the antioxidant activity of pea seedlings. Food Chem. 101:1753-1758. https://doi.org/10.1016/j.foodchem.2006.02.010
  28. Zhang, Y., N.P. Seeram, R. Lee, L. Feng, and D. Heber. 2008. Isolation and identification of strawberry phenolics with antioxidant and human cancer cell antiproliferative properties. J. Agric. Food Chem. 56:670-675. https://doi.org/10.1021/jf071989c
  29. Zheng, Y., S.Y. Wang, C.Y. Wang, and W. Zheng. 2007. Changes in strawberry phenolics, anthocyanins, and antioxidant capacity in response to high oxygen treatments. LWT - Food Sci. Technol. 40:49-57. https://doi.org/10.1016/j.lwt.2005.08.013

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  3. Effects of Supplemental LED Lighting on Productivity and Fruit Quality of Strawberry (Fragaria × ananassa Duch.) Grown on the Bottom Bed of the Two-Bed Bench System vol.27, pp.3, 2018, https://doi.org/10.12791/KSBEC.2018.27.3.199

Acknowledgement

Supported by : 농촌진흥청