Total Phenolics of Dried Platycodon grandiflorum and Its Effect on Growth of Human Cancer Cell Lines

건조 도라지 추출물 및 분획물의 총 페놀계 화합물 함량 및 인체 암세포 증식 억제효과

  • Hwang, Seong Yeon (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Choi, Hyang Mi (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Lim, Sun-Young (Division of Marine Environment & Bioscience, Korea Maritime University)
  • 황성연 (한국해양대학교 해양환경생명과학부) ;
  • 최향미 (한국해양대학교 해양환경생명과학부) ;
  • 임선영 (한국해양대학교 해양환경생명과학부)
  • Received : 2012.07.31
  • Accepted : 2012.12.11
  • Published : 2013.02.28


We investigated the effects of extracts from dried Platycodon grandiflorum on total phenolic content and growth of cancer cell lines (HT-29 and AGS). Total phenolic contents of acetone/methylene chloride (A+M) extract and methanol (MeOH) fraction were 4.53 and 27.22 TAE mg/100 g, respectively. Among the fractions, n-butanol (n-BuOH) fraction contained the highest phenolic content. Treatments of crude extracts and fractions significantly inhibited the growth of HT-29 and AGS cancer cell lines (p<0.05). Among the fractions, n-BuOH fraction exhibited the highest inhibitory effect and was then sub-fractionated by reverse phase flash column chromatography (rfc). The rfc 1-3 exhibited a higher inhibitory effect on proliferation of both cancer cells. The rfc 1 contained the highest phenol content. Our results showed that n-BuOH fraction possessed a potent inhibitory effect on proliferation of human cancer cells. We suggested that this anticancer activity was partially related to the content of phenolic compounds.


Supported by : 국토해양부


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